Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EXH44_RS01970 Genome accession   NZ_CP038145
Coordinates   422214..422678 (+) Length   154 a.a.
NCBI ID   WP_071610760.1    Uniprot ID   -
Organism   Actinobacillus indolicus strain AIFJ1607     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417214..427678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXH44_RS01960 (EXH44_01975) ppc 417944..420583 (+) 2640 WP_162856041.1 phosphoenolpyruvate carboxylase -
  EXH44_RS01965 (EXH44_01980) radA 420650..422026 (-) 1377 WP_162856042.1 DNA repair protein RadA -
  EXH44_RS01970 (EXH44_01985) pilA 422214..422678 (+) 465 WP_071610760.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  EXH44_RS01975 (EXH44_01990) pilB 422726..424111 (+) 1386 WP_162856043.1 GspE/PulE family protein Machinery gene
  EXH44_RS01980 (EXH44_01995) pilC 424104..425300 (+) 1197 WP_162856044.1 type II secretion system F family protein Machinery gene
  EXH44_RS01985 (EXH44_02000) - 425297..425965 (+) 669 WP_162856045.1 prepilin peptidase -
  EXH44_RS01990 (EXH44_02005) coaE 426063..426689 (+) 627 WP_162856046.1 dephospho-CoA kinase -
  EXH44_RS01995 (EXH44_02010) yacG 426686..426865 (+) 180 WP_162856047.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16420.00 Da        Isoelectric Point: 9.0758

>NTDB_id=351748 EXH44_RS01970 WP_071610760.1 422214..422678(+) (pilA) [Actinobacillus indolicus strain AIFJ1607]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVQADKTDVADAKYLKSISVASGTITVEGKGSIDGYGYTMTPKFANNNITWATSCRGSDISLFPANFCGTKATQ

Nucleotide


Download         Length: 465 bp        

>NTDB_id=351748 EXH44_RS01970 WP_071610760.1 422214..422678(+) (pilA) [Actinobacillus indolicus strain AIFJ1607]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCTGCACTCTCTGAGTTATTAAGTGCTTCTG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCAAGCGGATAAAACCGATGTTGCCGATGCGAAATATCTTAAATCTATTTCCGTCGCTTCGGGGACAATCACCGT
AGAAGGCAAAGGCAGCATTGATGGCTACGGCTACACAATGACCCCGAAATTTGCCAATAACAACATTACTTGGGCAACAA
GCTGTCGAGGTTCTGACATTAGTTTATTCCCTGCGAATTTCTGTGGGACAAAAGCCACACAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

94.118

99.351

0.935
  pilA Haemophilus influenzae Rd KW20

54.667

97.403

0.532
  pilA Haemophilus influenzae 86-028NP

53.691

96.753

0.519

Multiple sequence alignment