Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E3H47_RS01245 Genome accession   NZ_CP038022
Coordinates   266954..268663 (-) Length   569 a.a.
NCBI ID   WP_005023073.1    Uniprot ID   A0A062BXA9
Organism   Acinetobacter radioresistens strain DD78     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 261954..273663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3H47_RS01215 (E3H47_01215) - 262089..262412 (+) 324 WP_005016998.1 pyrimidine/purine nucleoside phosphorylase -
  E3H47_RS01220 (E3H47_01220) rlmB 262516..263268 (+) 753 WP_138000430.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E3H47_RS01225 (E3H47_01225) - 263293..264228 (+) 936 WP_005016993.1 DMT family transporter -
  E3H47_RS01230 (E3H47_01230) coaE 264225..264836 (-) 612 WP_005016991.1 dephospho-CoA kinase -
  E3H47_RS01235 (E3H47_01235) pilD 264838..265698 (-) 861 WP_005016989.1 A24 family peptidase Machinery gene
  E3H47_RS01240 (E3H47_01240) pilC 265698..266924 (-) 1227 WP_005016988.1 type II secretion system F family protein Machinery gene
  E3H47_RS01245 (E3H47_01245) pilB 266954..268663 (-) 1710 WP_005023073.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E3H47_RS01250 (E3H47_01250) tpiA 268952..269746 (+) 795 WP_005023075.1 triose-phosphate isomerase -
  E3H47_RS01255 (E3H47_01255) secG 269757..270086 (+) 330 WP_138000431.1 preprotein translocase subunit SecG -
  E3H47_RS01275 (E3H47_01275) rimP 270713..271237 (+) 525 WP_026444081.1 ribosome maturation factor RimP -
  E3H47_RS01280 (E3H47_01280) nusA 271278..272762 (+) 1485 WP_005016980.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63625.89 Da        Isoelectric Point: 5.0245

>NTDB_id=351490 E3H47_RS01245 WP_005023073.1 266954..268663(-) (pilB) [Acinetobacter radioresistens strain DD78]
MTVNFTPKFSGFIRRLVEDNHVSAAEMQNAVDTAKRNRQDIVPYLIDHFKLSPLLIAEFISIEFGEALFDLAVYDTVQIP
RDVVDEKLILKHRVLPLIKRGHTLFVATSNPTNIEALEAIRFNSKLNIEVVIVEHDKLQRTIEQNFSEESNFDFGDEELN
IDVSTSNLDEDDSQDNAPQEEEAPIVKYINKLLVDAIRIGASDLHFEPYEKYYRVRYRVDGVLRQVATPPLQLATRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFIEALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLSFSAALKSFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVAAFNIATSVNLVIAQRLARRLCNQCKVPAEIPRNSLLEMGFT
EDDLANPDFQVYQPVGCPECRDGYRGRVGIYEVMKVTPEISKIIMEDGNALEIAATAARLGFDNLRRSGLKKVMQGVTSL
QEINRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=351490 E3H47_RS01245 WP_005023073.1 266954..268663(-) (pilB) [Acinetobacter radioresistens strain DD78]
ATGACAGTTAATTTTACTCCAAAGTTTTCCGGTTTTATTCGCCGTCTGGTTGAGGATAACCACGTGTCTGCTGCTGAAAT
GCAGAATGCGGTCGATACTGCTAAAAGAAACCGGCAGGATATCGTGCCTTATCTGATCGATCATTTTAAGCTTTCACCCT
TACTGATTGCAGAATTTATCTCGATTGAATTTGGTGAAGCTTTATTCGATTTGGCTGTTTACGATACTGTCCAGATTCCA
CGAGATGTAGTTGATGAGAAACTGATACTCAAGCATCGGGTCTTGCCCCTCATTAAGCGTGGTCATACTCTGTTTGTTGC
TACCAGTAATCCCACCAATATTGAAGCTTTAGAAGCTATCCGGTTTAACAGTAAACTGAATATTGAAGTTGTTATTGTCG
AGCATGACAAACTGCAGCGTACTATTGAGCAGAATTTTTCTGAAGAAAGCAATTTTGATTTTGGTGATGAAGAACTCAAT
ATTGATGTTTCAACCAGCAATTTAGATGAGGACGACAGTCAAGATAATGCGCCTCAGGAAGAAGAAGCTCCAATTGTTAA
ATATATTAACAAGTTATTGGTAGATGCCATTCGTATTGGTGCATCAGACTTACATTTCGAGCCTTATGAAAAATATTACC
GAGTACGTTACCGGGTCGATGGCGTGTTAAGACAGGTGGCAACACCACCTTTACAGCTGGCCACCCGACTGGCTTCACGT
TTAAAGGTTATGTCACAGATGGATATTTCCGAAAAGCGCGTTCCCCAAGATGGCCGGATCAAATTAAAACTCTCTAAGAC
CAAAGCTATTGATTTCCGTGTGAACTCACTGCCTACACTATTTGGAGAAAAACTGGTTCTGCGTATTTTGGATCCGTCCA
GCGCCATGCTGGGTATTGATGCATTAGGCTATGAACCCGAGCAAAAAGCCCTGTTTATAGAAGCTTTAGAAAAGCCGCAA
GGTATGTTACTCATTACCGGGCCTACCGGTTCCGGTAAAACCGTATCGCTTTATACAGGCCTCAATATTTTAAACCGTGA
GGATACCAATATTTCAACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGTATTAACCAGGTCAATGTGAATAATAAAG
TCGGGTTAAGCTTCTCTGCCGCGCTAAAGTCATTCCTGCGTCAGGATCCAGATATTATTATGGTAGGTGAGATTCGTGAC
TTGGAAACAGCAGAGATTGCCATTAAAGCAGCTCAGACCGGTCACATGGTTATGTCTACCCTGCATACCAACAGCGCTCC
TGAAACATTAACACGCCTAAGAAATATGGGCGTAGCAGCATTTAATATTGCCACTTCAGTTAATCTGGTCATTGCACAGC
GTCTAGCTCGACGTTTATGCAACCAGTGCAAGGTACCAGCAGAGATTCCTAGAAATAGTCTGCTTGAGATGGGCTTTACC
GAAGATGACTTGGCCAACCCTGATTTTCAGGTTTATCAACCTGTCGGTTGCCCGGAATGTCGTGATGGTTATCGGGGTCG
TGTGGGTATCTATGAAGTAATGAAAGTTACACCGGAAATTTCAAAAATTATTATGGAAGACGGTAACGCCCTTGAAATAG
CAGCAACAGCAGCACGCCTGGGTTTTGATAACTTGCGTCGTTCTGGCCTAAAGAAAGTCATGCAAGGTGTAACCTCTTTA
CAAGAAATTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A062BXA9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.383

99.121

0.807
  pilB Acinetobacter baylyi ADP1

78.33

98.946

0.775
  pilB Legionella pneumophila strain ERS1305867

53.676

95.606

0.513
  pilF Neisseria gonorrhoeae MS11

49.644

98.77

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

92.091

0.476
  pilB Vibrio cholerae strain A1552

50.478

91.916

0.464
  pilB Vibrio campbellii strain DS40M4

50.287

91.916

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

34.516

100

0.376
  pilF Thermus thermophilus HB27

36.525

99.121

0.362

Multiple sequence alignment