Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AHTJR_RS14050 Genome accession   NZ_CP038009
Coordinates   2990555..2992270 (+) Length   571 a.a.
NCBI ID   WP_134252944.1    Uniprot ID   A0A4P7B8A8
Organism   Acinetobacter haemolyticus strain TJR01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2985555..2997270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AHTJR_RS14015 (AHTJR_14015) nusA 2986364..2987848 (-) 1485 WP_004637688.1 transcription termination factor NusA -
  AHTJR_RS14020 (AHTJR_14020) rimP 2987886..2988410 (-) 525 WP_005083847.1 ribosome maturation factor RimP -
  AHTJR_RS14040 (AHTJR_14040) secG 2989045..2989374 (-) 330 WP_004637686.1 preprotein translocase subunit SecG -
  AHTJR_RS14045 (AHTJR_14045) tpiA 2989387..2990178 (-) 792 WP_075316302.1 triose-phosphate isomerase -
  AHTJR_RS14050 (AHTJR_14050) pilB 2990555..2992270 (+) 1716 WP_134252944.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AHTJR_RS14055 (AHTJR_14055) pilC 2992295..2993524 (+) 1230 WP_134252945.1 type II secretion system F family protein Machinery gene
  AHTJR_RS14060 (AHTJR_14060) pilD 2993537..2994397 (+) 861 WP_134252946.1 prepilin peptidase Machinery gene
  AHTJR_RS14065 (AHTJR_14065) coaE 2994399..2995004 (+) 606 WP_134252947.1 dephospho-CoA kinase -
  AHTJR_RS14070 (AHTJR_14070) - 2995001..2995912 (-) 912 WP_134252948.1 DMT family transporter -
  AHTJR_RS14080 (AHTJR_14080) rlmB 2995952..2996698 (-) 747 WP_004637678.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AHTJR_RS14085 (AHTJR_14085) - 2996807..2997133 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63302.50 Da        Isoelectric Point: 5.1503

>NTDB_id=351250 AHTJR_RS14050 WP_134252944.1 2990555..2992270(+) (pilB) [Acinetobacter haemolyticus strain TJR01]
MSGLHTSPKFSGFIRRLVEDGHMSASTMQTAIDTAKKTQQDIVGYLIEHHRISQLTIAESISAEFGEPIFDLAVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPIIVEHSKLEKLIEQQFGDSGSFDFGETED
FDLDIDANQDSQKDESDEAPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFMEALEK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPIEIPKQSLLELG
FTEQDLADPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=351250 AHTJR_RS14050 WP_134252944.1 2990555..2992270(+) (pilB) [Acinetobacter haemolyticus strain TJR01]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTAGTCGAAGATGGTCATATGTCTGCATCGAC
AATGCAAACTGCAATCGATACAGCAAAAAAAACACAACAAGATATTGTTGGATATTTAATTGAACATCATCGTATCTCTC
AACTCACCATTGCAGAGTCAATCTCGGCAGAGTTTGGCGAACCTATTTTTGATTTAGCTGTTTTTGATACTGCTTTATTA
CCTCGTGAGCTTGCTGACAGCAAATTAATTCAAAAGCATCGGGTTGCCCCTCTCATTCAACGCGGACAAATTCTTTATGT
CGCGACAAGCAATCCAACCAATATCGACGCGCTTGATGCGATTCGCTTTAACACCAAGCTCAATATTGAACCAATTATCG
TTGAACACAGTAAACTCGAAAAACTGATTGAACAGCAGTTTGGTGACTCTGGAAGTTTTGATTTTGGTGAAACCGAAGAT
TTTGACTTAGATATTGATGCCAACCAAGACTCGCAAAAAGATGAAAGTGATGAAGCACCTCAAGGGGATGAATCACCAAT
TGTAAAATACATCAACAAGTTACTGATTGATGCGATTCGCATGGGTGCATCTGATCTACATTTTGAACCCTATGAAAAAA
CCTATCGTGTTCGTTATCGTGTAGATGGTGTCTTAAGACAAATTGCGACACCGCCGTTGCAAATGGCAAATCGCTTAGCT
TCCCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGTCGTATTAAGCTCAAACTTTC
AAAAACCAAAGCGATTGACTTTCGTGTCAACTCACTGCCTACCCTATTTGGTGAAAAACTAGTACTCCGTATTCTGGATC
CTTCCAGCGCGATGCTCGGAATTGAAGCACTCGGTTACGAGGATGAACAAAAAGCTCTATTTATGGAGGCGCTTGAAAAA
CCTCAAGGCATGCTGTTAATTACTGGCCCAACAGGTTCTGGTAAAACTGTGTCGTTATATACAGGTCTCAACATTCTAAA
TACTGAAGACACCAATATTTCTACTGCTGAAGACCCAGTGGAAATTAACTTAGAAGGAATCAATCAGGTTAACGTCAACA
CCAAGACAGGTCTTACCTTCTCTGCTGCCCTTAAAGCATTCCTACGCCAAGATCCAGACATTATTATGGTCGGTGAGATT
CGTGACCTAGAAACAGCAGAAATTGCAATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCCACGCTACATACCAATAG
TGCACCTGAAACACTGACGCGTTTACGCAATATGGGTGTTCCTTCTTTCAATATCGCAACTTCGGTCAATCTTGTGATTG
CACAACGCTTAGCCCGTCGTTTATGCTCACACTGTAAAGCACCTATAGAAATCCCCAAACAAAGCTTATTAGAACTCGGC
TTTACTGAACAGGATTTAGCTGACCCTGAATTGCAGATCTTTCAACCAGTGGGCTGCTCAGAATGTCGTGAAGGCTATAA
AGGTCGTGTCGGTATCTATGAGGTCATGAAAGTGACGCCCGAGATCTCTAAAATTATTATGGAGGATGGTAATGCTTTAC
AAATCGCAGCAGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGCTCAGGTTTAAAGAAGGTCATGCAAGGCATTACC
TCATTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P7B8A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.312

100

0.823
  pilB Acinetobacter baylyi ADP1

77.583

100

0.776
  pilB Legionella pneumophila strain ERS1305867

53.396

92.82

0.496
  pilF Neisseria gonorrhoeae MS11

49.037

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

97.548

0.482
  pilB Vibrio cholerae strain A1552

48.82

96.497

0.471
  pilB Vibrio campbellii strain DS40M4

51.06

90.893

0.464
  pilF Thermus thermophilus HB27

37.655

98.599

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

33.984

100

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.91

93.17

0.363

Multiple sequence alignment