Detailed information NTG0351252

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AHTJR_RS14060
• Genome accession: NZ_CP038009
• Coordinates: 2993537..2994397 (+)
• Length: 286 a.a.
• NCBI ID: WP_134252946.1
• Uniprot ID: A0A4P7B6X2
• Organism: Acinetobacter haemolyticus strain TJR01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2988537..2999397

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AHTJR_RS14040 (AHTJR_14040)	secG	2989045..2989374 (-)	330	WP_004637686.1	preprotein translocase subunit SecG	-
AHTJR_RS14045 (AHTJR_14045)	tpiA	2989387..2990178 (-)	792	WP_075316302.1	triose-phosphate isomerase	-
AHTJR_RS14050 (AHTJR_14050)	pilB	2990555..2992270 (+)	1716	WP_134252944.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AHTJR_RS14055 (AHTJR_14055)	pilC	2992295..2993524 (+)	1230	WP_134252945.1	type II secretion system F family protein	Machinery gene
AHTJR_RS14060 (AHTJR_14060)	pilD	2993537..2994397 (+)	861	WP_134252946.1	prepilin peptidase	Machinery gene
AHTJR_RS14065 (AHTJR_14065)	coaE	2994399..2995004 (+)	606	WP_134252947.1	dephospho-CoA kinase	-
AHTJR_RS14070 (AHTJR_14070)	-	2995001..2995912 (-)	912	WP_134252948.1	DMT family transporter	-
AHTJR_RS14080 (AHTJR_14080)	rlmB	2995952..2996698 (-)	747	WP_004637678.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
AHTJR_RS14085 (AHTJR_14085)	-	2996807..2997133 (-)	327	WP_004637677.1	pyrimidine/purine nucleoside phosphorylase	-
AHTJR_RS14090 (AHTJR_14090)	recN	2997467..2999128 (+)	1662	WP_134252950.1	DNA repair protein RecN	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32028.28 Da
• Isoelectric Point: 6.4294

>NTDB_id=351252 AHTJR_RS14060 WP_134252946.1 2993537..2994397(+) (pilD) [Acinetobacter haemolyticus strain TJR01]
MQQFLNYFIENPTALYLAVGLFSLCIGSFLNVVIFRTPRMMEQEWQQECQMLLHPEQPIIEEEKLTLSQPASTCPKCHTP
IRWYQNIPVTSWLVLRGKCGACQNPISIRYPLIELLTMGCSLVVVAVFGATLQMLFGLVLTWVLITLTFIDFDTQLLPDR
FTLPLAALGLAINSFAIYTTANSAIWGYVIGFLCLWIVYYLFKIFTGKEGMGYGDFKLLAALGAWMGPMLLPLIVLLSSV
VGAIIGIILLKMRGENQPFAFGPYIAIAGWIAFLWGDQIMKVYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=351252 AHTJR_RS14060 WP_134252946.1 2993537..2994397(+) (pilD) [Acinetobacter haemolyticus strain TJR01]
ATGCAGCAATTCTTAAATTATTTTATCGAAAACCCAACTGCACTTTATCTTGCAGTTGGGCTTTTTAGTTTATGTATTGG
TAGCTTTCTGAATGTTGTTATTTTCCGAACGCCAAGAATGATGGAACAGGAATGGCAACAAGAATGCCAAATGCTACTCC
ATCCAGAACAACCTATCATTGAAGAAGAAAAGCTGACTTTAAGTCAACCCGCATCAACTTGCCCAAAGTGCCACACCCCT
ATCCGCTGGTATCAAAATATCCCAGTGACCAGCTGGTTAGTACTCCGTGGTAAATGCGGTGCCTGTCAAAACCCGATTAG
TATTCGTTACCCATTGATCGAGTTATTGACCATGGGATGCTCTCTAGTGGTCGTTGCTGTTTTTGGTGCAACCCTCCAAA
TGCTATTCGGTTTAGTTCTGACTTGGGTGTTAATTACCCTTACTTTTATTGATTTTGATACACAATTGCTTCCAGACCGC
TTTACACTCCCACTGGCAGCACTCGGTTTAGCCATTAATAGTTTTGCGATCTACACCACAGCAAACTCAGCAATTTGGGG
TTATGTGATTGGCTTCCTTTGCCTTTGGATTGTTTATTATCTTTTTAAAATCTTTACTGGCAAAGAGGGTATGGGCTATG
GCGATTTTAAATTACTGGCTGCATTAGGGGCTTGGATGGGTCCAATGCTCTTGCCTTTAATTGTGTTACTTTCTTCTGTT
GTCGGCGCAATTATTGGTATCATCTTATTGAAAATGCGCGGTGAAAATCAGCCCTTTGCTTTTGGCCCCTATATTGCAAT
TGCAGGCTGGATCGCATTTTTATGGGGTGATCAGATTATGAAAGTTTATCTAGGTGGCTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: A0A4P7B6X2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	84.266	100	0.843
pilD	Acinetobacter baumannii D1279779	83.217	100	0.832
pilD	Vibrio cholerae strain A1552	55.97	93.706	0.524
pilD	Vibrio campbellii strain DS40M4	53.759	93.007	0.5
pilD	Neisseria gonorrhoeae MS11	47.253	95.455	0.451