Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   E2H98_RS04815 Genome accession   NZ_CP037953
Coordinates   1045233..1046516 (+) Length   427 a.a.
NCBI ID   WP_133593127.1    Uniprot ID   A0A4R6UFD1
Organism   Permianibacter aggregans strain HW001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040233..1051516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2H98_RS04800 (E2H98_04790) - 1041589..1042311 (-) 723 WP_133593133.1 hypothetical protein -
  E2H98_RS04805 (E2H98_04795) - 1042723..1043190 (+) 468 WP_133593131.1 pilin -
  E2H98_RS04810 (E2H98_04800) pilB 1043288..1045000 (+) 1713 WP_133593129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E2H98_RS04815 (E2H98_04805) pilC 1045233..1046516 (+) 1284 WP_133593127.1 type II secretion system F family protein Machinery gene
  E2H98_RS04820 (E2H98_04810) pilD 1046519..1047412 (+) 894 WP_133593125.1 A24 family peptidase Machinery gene
  E2H98_RS04825 (E2H98_04815) coaE 1047423..1048031 (+) 609 WP_133593123.1 dephospho-CoA kinase -
  E2H98_RS04830 (E2H98_04820) - 1048028..1048777 (+) 750 WP_133593121.1 cell division protein ZapD -
  E2H98_RS04835 (E2H98_04825) yacG 1048793..1048987 (+) 195 WP_133593119.1 DNA gyrase inhibitor YacG -
  E2H98_RS04840 (E2H98_04830) - 1049015..1050016 (-) 1002 WP_133593117.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 427 a.a.        Molecular weight: 46526.91 Da        Isoelectric Point: 10.0393

>NTDB_id=350936 E2H98_RS04815 WP_133593127.1 1045233..1046516(+) (pilC) [Permianibacter aggregans strain HW001]
MALQAVKKEVPVKPRPGAITRQKQTVEKQKTYVWIGTDKKGQKLKGEMQASNEALVKAHLRKQGLVPVKVNRKAADLFAP
RKKPITASDISLVSRQLATMMKAGVPLVQSFEIIGKGNDKPAVQELMLNIKADVEAGAPFHESLRKHPKEFDPLFCDLVA
AGEQSGALEQMLDRIATYKEKTERIKAKIKKALFYPIAVLVVAVIVTAILLLFVVPMFEDMFKSFGADLPAFTQFVLGIS
RSLQETWYVYLIAIVAASFGFGYLNRTSQKFRDGKERVILKMPVVGMILHKAAIARYARTLSTTFAAGVPLVEALESAAG
ASGNVVYRNAILKIRDDVTTGTQMNLAMQSSGVFPNMVVQMVAIGEESGSLDSMLSKVADIYEAEVDDAVDSLSALLEPI
IMAVLGVVVGGLIIAMYLPIFNLGKAI

Nucleotide


Download         Length: 1284 bp        

>NTDB_id=350936 E2H98_RS04815 WP_133593127.1 1045233..1046516(+) (pilC) [Permianibacter aggregans strain HW001]
ATGGCATTGCAAGCGGTTAAAAAAGAAGTTCCGGTCAAACCACGGCCAGGGGCGATTACCCGCCAAAAGCAGACTGTTGA
AAAACAGAAAACCTACGTTTGGATTGGAACCGATAAAAAAGGCCAGAAACTCAAAGGTGAAATGCAGGCCTCCAATGAAG
CTTTGGTCAAAGCCCATCTTAGAAAACAAGGCCTGGTTCCAGTTAAGGTCAATCGTAAAGCAGCGGATCTGTTTGCCCCC
CGAAAGAAACCCATAACGGCATCCGATATTTCTCTCGTGTCACGCCAATTAGCGACAATGATGAAGGCGGGCGTGCCATT
GGTTCAGAGTTTTGAAATTATCGGGAAAGGGAATGATAAGCCAGCAGTTCAGGAGTTGATGCTGAACATCAAAGCGGATG
TTGAAGCCGGTGCACCTTTCCATGAGTCGCTTCGTAAACATCCTAAAGAGTTCGACCCATTATTTTGCGACCTCGTGGCA
GCAGGGGAGCAGTCTGGTGCACTGGAGCAAATGCTTGACCGGATTGCCACGTATAAGGAAAAAACGGAGCGCATCAAAGC
AAAAATTAAGAAGGCGCTTTTTTATCCGATAGCAGTATTAGTTGTTGCCGTAATCGTGACAGCCATTTTGTTGCTTTTCG
TTGTTCCCATGTTCGAGGACATGTTCAAGAGCTTTGGCGCAGACTTGCCGGCCTTTACACAGTTTGTATTAGGCATATCG
AGGTCTCTTCAGGAAACATGGTATGTGTACCTCATTGCCATCGTTGCTGCCTCATTCGGATTTGGTTACTTGAATCGAAC
CTCTCAGAAATTTCGTGATGGTAAAGAGCGCGTAATACTGAAGATGCCAGTGGTGGGTATGATTCTTCATAAAGCAGCCA
TTGCGCGTTATGCCCGCACACTTTCCACGACGTTTGCGGCGGGTGTGCCGTTAGTCGAAGCGTTGGAGTCAGCAGCAGGC
GCCTCTGGTAATGTGGTTTATCGGAACGCAATCTTGAAGATTCGCGATGACGTAACCACTGGTACTCAAATGAATTTGGC
CATGCAATCGTCAGGTGTTTTTCCAAATATGGTTGTACAGATGGTTGCTATCGGCGAAGAATCAGGCTCTTTGGACAGCA
TGCTGTCCAAAGTTGCTGATATTTACGAAGCCGAAGTCGATGATGCCGTCGATTCACTCAGCGCGCTGTTAGAGCCGATT
ATCATGGCTGTGCTTGGCGTGGTTGTTGGCGGATTGATTATCGCCATGTATCTACCAATCTTTAACTTGGGTAAAGCCAT
CTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(298-419)

Type II secretion system (T2SS), protein F (T2SSF)

(94-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4R6UFD1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

59.901

94.614

0.567
  pilC Legionella pneumophila strain ERS1305867

57.431

92.974

0.534
  pilC Acinetobacter baumannii D1279779

57.035

93.208

0.532
  pilC Acinetobacter baylyi ADP1

55.416

92.974

0.515
  pilC Vibrio cholerae strain A1552

45.178

92.272

0.417
  pilG Neisseria gonorrhoeae MS11

43.781

94.145

0.412
  pilG Neisseria meningitidis 44/76-A

43.532

94.145

0.41
  pilC Vibrio campbellii strain DS40M4

43.038

92.506

0.398

Multiple sequence alignment