Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E2H98_RS04810 Genome accession   NZ_CP037953
Coordinates   1043288..1045000 (+) Length   570 a.a.
NCBI ID   WP_133593129.1    Uniprot ID   -
Organism   Permianibacter aggregans strain HW001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038288..1050000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E2H98_RS04785 (E2H98_04775) nadC 1038362..1039243 (+) 882 WP_198325301.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E2H98_RS04790 (E2H98_04780) - 1039220..1041328 (-) 2109 WP_133593135.1 sulfatase-like hydrolase/transferase -
  E2H98_RS04800 (E2H98_04790) - 1041589..1042311 (-) 723 WP_133593133.1 hypothetical protein -
  E2H98_RS04805 (E2H98_04795) - 1042723..1043190 (+) 468 WP_133593131.1 pilin -
  E2H98_RS04810 (E2H98_04800) pilB 1043288..1045000 (+) 1713 WP_133593129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E2H98_RS04815 (E2H98_04805) pilC 1045233..1046516 (+) 1284 WP_133593127.1 type II secretion system F family protein Machinery gene
  E2H98_RS04820 (E2H98_04810) pilD 1046519..1047412 (+) 894 WP_133593125.1 A24 family peptidase Machinery gene
  E2H98_RS04825 (E2H98_04815) coaE 1047423..1048031 (+) 609 WP_133593123.1 dephospho-CoA kinase -
  E2H98_RS04830 (E2H98_04820) - 1048028..1048777 (+) 750 WP_133593121.1 cell division protein ZapD -
  E2H98_RS04835 (E2H98_04825) yacG 1048793..1048987 (+) 195 WP_133593119.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62502.54 Da        Isoelectric Point: 5.0327

>NTDB_id=350935 E2H98_RS04810 WP_133593129.1 1043288..1045000(+) (pilB) [Permianibacter aggregans strain HW001]
MSLTPSKIALGGLAKRLVQDGVLDESIAQESVAQSQKTKQSLISYLVQGKHAKAHVLANIASQEFGTPLFDLDSMDVDQI
PKELVDENIIRNQRTIPLFKRGNRLFIAISDPTNLEALDKARFQTGMSVEGILVEDDKLGKFIDKILDEKESGGLSEFDD
EDLDNLDIQGGDDDPKDEGDAGGDDAPIVRFINKLLIDAIKKGASDLHFEPYEKEYRVRFRVDGVLKEMASPPVNLASRL
AARLKVMSRLDIAERRVPQDGRIKMKLSKNKAIDFRVSTLPTLWGEKVVMRILDPSSAMLGIDALGYEPVQKKSFMDAIA
KPQGMVLVTGPTGSGKTVSLYTAVNILNDEETNISTAEDPVEINLPGVNQVQVNPRAGLTFPMALRAFLRQDPDVILVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLVNMGVAPFNIATTVHLIIAQRLARRLCSHCKKPVKIPAEALLKE
GFREDEAETLTLYEPVGCDNCNNGYKGRVGIYEVMPVSNEISRIVMRGGNAIEIADQMRAEGISDLRSSGLEKARQGVSS
LQEINRVTSD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=350935 E2H98_RS04810 WP_133593129.1 1043288..1045000(+) (pilB) [Permianibacter aggregans strain HW001]
ATGAGTTTAACGCCATCAAAAATCGCCCTAGGGGGCCTGGCCAAACGCTTGGTGCAAGACGGCGTACTGGACGAAAGTAT
TGCGCAAGAGTCCGTTGCACAATCACAAAAGACCAAGCAGTCATTGATTAGCTACCTTGTACAGGGAAAACATGCCAAAG
CACATGTGCTAGCGAATATTGCCTCTCAGGAGTTTGGCACTCCGTTGTTCGATCTGGACTCCATGGATGTTGACCAGATA
CCCAAAGAGTTAGTCGACGAAAACATTATCCGCAATCAACGGACAATTCCCCTGTTCAAACGTGGGAATCGGCTCTTCAT
AGCTATATCTGACCCTACCAACCTTGAGGCATTGGACAAGGCTAGATTCCAAACCGGCATGTCAGTCGAAGGTATCCTGG
TCGAAGATGACAAACTCGGAAAATTTATCGACAAAATTCTCGATGAGAAAGAAAGCGGCGGACTGTCCGAATTCGATGAT
GAGGACTTGGATAATCTCGACATTCAAGGCGGAGATGATGATCCTAAGGACGAGGGGGATGCGGGTGGTGACGATGCGCC
GATCGTTCGCTTTATCAACAAGCTCTTAATCGACGCTATCAAAAAGGGCGCCTCGGACCTACACTTTGAGCCTTATGAAA
AGGAATACCGCGTTCGTTTCAGGGTTGACGGCGTACTCAAAGAGATGGCTAGTCCACCGGTAAATCTTGCCAGCCGCTTG
GCCGCACGTTTAAAGGTAATGTCTCGTTTGGATATCGCTGAACGGCGCGTACCACAGGATGGCCGGATCAAGATGAAGTT
GAGTAAGAACAAGGCCATCGATTTTCGTGTTTCGACCTTGCCCACACTCTGGGGCGAAAAGGTCGTTATGCGTATTCTGG
ACCCAAGCTCTGCGATGCTCGGTATCGATGCGCTGGGTTATGAGCCAGTGCAGAAAAAGTCCTTTATGGACGCGATCGCC
AAACCACAGGGCATGGTATTGGTAACTGGTCCAACTGGTTCAGGGAAAACCGTTTCGCTTTATACCGCTGTAAACATTCT
TAACGATGAGGAAACGAATATTTCAACGGCCGAAGACCCGGTCGAGATTAACCTGCCGGGCGTTAACCAAGTTCAAGTCA
ATCCAAGAGCCGGATTGACGTTCCCTATGGCGCTGCGCGCCTTCCTGCGCCAAGATCCTGACGTAATTCTGGTTGGCGAA
ATTCGAGACCTCGAAACGGCCGAGATTGCTATTAAGGCAGCTCAAACAGGACACTTGGTGCTCTCGACTCTTCATACCAA
CAGTGCTCCAGAAACCTTGACACGCTTGGTAAACATGGGAGTTGCGCCATTCAATATTGCTACAACAGTACACCTAATTA
TCGCTCAACGCTTGGCTCGGCGCCTTTGCTCTCATTGCAAAAAGCCGGTGAAAATCCCGGCGGAAGCGCTGCTTAAAGAA
GGTTTCCGAGAGGATGAAGCAGAGACCTTGACGCTCTACGAGCCTGTTGGTTGTGACAACTGTAACAATGGTTATAAAGG
ACGGGTAGGTATATACGAGGTTATGCCCGTATCTAACGAAATTAGTAGGATTGTCATGCGTGGTGGGAATGCAATTGAAA
TCGCCGATCAAATGAGAGCTGAAGGCATTTCTGACTTGCGTAGTTCAGGGCTGGAGAAGGCCAGACAAGGCGTTAGTAGT
CTGCAGGAAATAAACCGAGTAACGTCTGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.243

100

0.609
  pilB Acinetobacter baylyi ADP1

60.351

100

0.604
  pilB Legionella pneumophila strain ERS1305867

55.304

100

0.558
  pilB Vibrio cholerae strain A1552

50.98

98.421

0.502
  pilF Neisseria gonorrhoeae MS11

50.444

98.772

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

49.283

97.895

0.482
  pilB Vibrio campbellii strain DS40M4

48.845

98.772

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

36.617

100

0.391
  pilF Thermus thermophilus HB27

38.53

97.895

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

92.456

0.368

Multiple sequence alignment