Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EYZ66_RS10635 Genome accession   NZ_CP036423
Coordinates   2375995..2377731 (+) Length   578 a.a.
NCBI ID   WP_009575691.1    Uniprot ID   F3L1Q6
Organism   Aequoribacter fuscus strain IMCC3088     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2370995..2382731
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EYZ66_RS10620 (EYZ66_10620) - 2371136..2372305 (+) 1170 WP_009575694.1 nucleotide sugar dehydrogenase -
  EYZ66_RS10625 (EYZ66_10625) - 2372540..2374705 (+) 2166 WP_009575693.1 malate synthase G -
  EYZ66_RS10630 (EYZ66_10630) - 2375276..2375743 (-) 468 WP_040816565.1 pilin -
  EYZ66_RS10635 (EYZ66_10635) pilB 2375995..2377731 (+) 1737 WP_009575691.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EYZ66_RS10640 (EYZ66_10640) pilC 2377746..2378957 (+) 1212 WP_160195670.1 type II secretion system F family protein Machinery gene
  EYZ66_RS10645 (EYZ66_10645) pilD 2378976..2379833 (+) 858 WP_009575689.1 A24 family peptidase Machinery gene
  EYZ66_RS10650 (EYZ66_10650) coaE 2379841..2380431 (+) 591 WP_009575688.1 dephospho-CoA kinase -
  EYZ66_RS10655 (EYZ66_10655) mutT 2380414..2380836 (-) 423 WP_009575687.1 8-oxo-dGTP diphosphatase MutT -
  EYZ66_RS10660 (EYZ66_10660) argJ 2380817..2382013 (-) 1197 WP_009575686.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63241.61 Da        Isoelectric Point: 5.6951

>NTDB_id=348848 EYZ66_RS10635 WP_009575691.1 2375995..2377731(+) (pilB) [Aequoribacter fuscus strain IMCC3088]
MNQATSPTASKARLGGLAGRLIMDGLITADQAMEAQKEATAARIPFVQHLVEAKGIDSAKIAAAASSEFGVPLMDLRAFD
NTQFPEDLVDIKLIQRHHALPLFKRGKRLFIALADPTNLQALDEIKFHTGINTEAIIVDQKALSAAISHATETKDAIEQL
GDLESGQFEDLEVEAIDENDRADGDLNMEADDTPIVRFVNKVLLDAIKAGASDIHFEPYEHTYRVRFRADGILKEVVQPP
KALAPRLAARLKVMSRMDISERRVPQDGRIQMRLSKTRAIDMRVNTLPTLFGEKIVLRILDPTSATLGIDALGYEPHQKK
LFTDALDKPQGMILVTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLDGINQVPVNPKVGLNFADALRSFLRQDP
DVIMVGEIRDLETAEISIKAAQTGHLVLSTLHTNSAAETITRLLNMGVPAFNLATAVTLIIAQRLARRLCSKCAEPIDEL
PEESLLELGFTKEMLKGATIKKAVGCESCNHGYKGRTGIYEVVQITQTIARMILEGKNSMEIADEARKEGFHSLRTSALR
KVSQGLISLEEANRVTVD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=348848 EYZ66_RS10635 WP_009575691.1 2375995..2377731(+) (pilB) [Aequoribacter fuscus strain IMCC3088]
GTGAACCAAGCCACATCACCTACAGCAAGCAAAGCGCGATTGGGTGGCCTAGCAGGCCGCTTAATTATGGACGGCCTCAT
CACAGCCGACCAGGCTATGGAGGCGCAAAAAGAAGCGACCGCTGCGCGCATTCCATTTGTACAGCACCTTGTTGAAGCGA
AAGGTATTGATAGCGCCAAGATTGCCGCCGCTGCATCGTCCGAGTTTGGGGTACCGTTGATGGACCTGCGCGCGTTTGAT
AATACTCAGTTTCCAGAAGACTTGGTTGACATCAAGCTCATCCAAAGACATCACGCGCTTCCCTTATTTAAACGCGGTAA
GCGATTGTTCATCGCACTGGCAGACCCAACCAACCTGCAGGCTCTGGACGAAATTAAGTTCCACACTGGCATTAACACCG
AAGCCATTATCGTCGACCAAAAAGCGTTATCAGCCGCCATCTCGCACGCCACCGAAACCAAAGATGCGATAGAACAGCTT
GGCGACTTAGAGAGTGGTCAGTTTGAAGATCTTGAGGTCGAAGCGATCGACGAAAACGATCGCGCTGACGGCGACCTGAA
TATGGAAGCGGACGACACCCCCATCGTCCGTTTTGTAAACAAAGTGTTACTGGATGCCATTAAAGCCGGAGCCTCTGACA
TTCATTTTGAGCCTTACGAGCACACCTATCGCGTGCGCTTCCGTGCCGATGGCATCCTGAAAGAGGTCGTGCAACCGCCC
AAGGCACTGGCGCCCCGTTTGGCGGCACGATTAAAAGTCATGTCTCGAATGGACATTTCTGAGCGACGAGTTCCTCAGGA
TGGACGCATCCAAATGCGTCTGTCCAAGACACGCGCCATTGATATGCGGGTGAACACCCTGCCCACCTTATTTGGCGAAA
AGATCGTACTGCGTATTTTGGACCCCACCAGCGCGACCTTGGGAATTGATGCACTGGGTTACGAACCGCATCAGAAAAAA
CTGTTCACCGATGCGCTCGACAAGCCGCAAGGCATGATCCTAGTTACGGGACCCACAGGTTCGGGTAAAACGGTATCGCT
GTACACGGGCCTTAATATTCTTAATACCCCCGAGCGCAACATCTCGACCGCGGAAGATCCGGTCGAGATCAACTTGGATG
GCATTAACCAAGTACCGGTTAACCCGAAGGTGGGCCTGAACTTTGCCGACGCGCTGCGCTCGTTTTTGCGCCAAGACCCA
GACGTGATCATGGTCGGGGAGATCCGTGACCTGGAAACGGCCGAAATCTCAATTAAAGCCGCCCAAACAGGTCACTTGGT
GCTATCGACACTGCACACGAATAGCGCGGCCGAAACGATCACTCGCTTATTAAATATGGGCGTACCCGCCTTTAACTTGG
CAACAGCCGTGACCTTGATTATTGCGCAGCGTTTGGCTCGGCGCTTGTGTTCAAAATGCGCCGAGCCCATTGATGAATTA
CCTGAGGAAAGCCTGCTGGAACTGGGTTTTACCAAAGAGATGCTCAAGGGCGCCACCATCAAAAAAGCGGTTGGCTGCGA
GTCGTGTAACCATGGCTACAAGGGTCGAACGGGCATTTACGAAGTTGTGCAAATTACCCAAACCATTGCGCGCATGATTC
TGGAAGGTAAAAACTCGATGGAGATCGCCGATGAAGCGCGCAAAGAGGGCTTCCACAGCCTTAGGACCTCGGCTTTAAGG
AAAGTGTCGCAGGGCTTAATCAGCTTAGAAGAAGCAAACCGAGTCACGGTAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-154)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F3L1Q6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.168

100

0.573
  pilB Acinetobacter baumannii D1279779

56.87

99.481

0.566
  pilB Legionella pneumophila strain ERS1305867

55.36

98.443

0.545
  pilB Vibrio cholerae strain A1552

52.441

95.675

0.502
  pilB Vibrio campbellii strain DS40M4

50.709

97.578

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

50.719

96.194

0.488
  pilF Neisseria gonorrhoeae MS11

48.325

98.097

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

40.485

92.734

0.375

Multiple sequence alignment