Detailed information NTG0348839

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EYZ66_RS01725
• Genome accession: NZ_CP036423
• Coordinates: 394795..395922 (+)
• Length: 375 a.a.
• NCBI ID: WP_009576863.1
• Uniprot ID: F3L4U6
• Organism: Aequoribacter fuscus strain IMCC3088
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 389795..400922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EYZ66_RS01715 (EYZ66_01710)	-	390650..391684 (+)	1035	WP_160195568.1	efflux RND transporter periplasmic adaptor subunit	-
EYZ66_RS01720 (EYZ66_01715)	-	391688..394798 (+)	3111	WP_160195569.1	efflux RND transporter permease subunit	-
EYZ66_RS01725 (EYZ66_01720)	pilU	394795..395922 (+)	1128	WP_009576863.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EYZ66_RS01730 (EYZ66_01725)	-	395964..397316 (-)	1353	WP_158027015.1	VWA domain-containing protein	-
EYZ66_RS01735 (EYZ66_01730)	-	397340..398287 (-)	948	WP_009576865.1	MoxR family ATPase	-
EYZ66_RS01740 (EYZ66_01735)	moaA	398318..399307 (-)	990	WP_009576866.1	GTP 3',8-cyclase MoaA	-
EYZ66_RS01745 (EYZ66_01740)	-	399401..400444 (+)	1044	WP_040817345.1	hypothetical protein	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 42044.90 Da
• Isoelectric Point: 4.9289

>NTDB_id=348839 EYZ66_RS01725 WP_009576863.1 394795..395922(+) (pilU) [Aequoribacter fuscus strain IMCC3088]
MSRIQEWLKTLARDKGSDLYLSTGAPPCAKFQGKLKPIANEVLKPGEIRAIAYELMDDTQIADFERELEMNLATSIAGYG
RFRVNIFIQRSEVSIVARNIVAEIPNWQDLRLPEIMLDVIMRKRGLVLFVGGTGSGKSTSLAALIDYRNSNSNGHIVTVE
DPVEYVHRHKKCIVNQREVGVDTRSWHNALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLCLSTLHANNANQALDR
IVNFFPEERRPQLMMDLSQNLQAFVSQRLIPTVDNKRVAAIEVLLGTPLVSDIILRGQFDGLKEVMEKSENLGMKTFDQA
CFELYQEGLISEEEALRNADSANNVRLKIKFAGSTVGGDFEDEDDLGLSLDGEDF

Nucleotide

• Length: 1128 bp

>NTDB_id=348839 EYZ66_RS01725 WP_009576863.1 394795..395922(+) (pilU) [Aequoribacter fuscus strain IMCC3088]
ATGAGTCGCATACAGGAATGGTTGAAAACGTTAGCTCGAGATAAAGGGTCAGATCTGTACTTAAGTACAGGCGCGCCGCC
TTGCGCCAAGTTTCAGGGCAAGTTAAAGCCTATTGCCAACGAAGTCCTAAAGCCCGGCGAGATTCGAGCAATTGCCTACG
AGTTGATGGACGATACCCAAATAGCGGACTTTGAGCGAGAGCTCGAAATGAACTTAGCGACCTCGATTGCGGGCTATGGC
CGTTTTCGCGTGAACATTTTCATTCAGCGCTCGGAAGTGAGTATCGTCGCTCGTAACATTGTCGCCGAAATTCCCAACTG
GCAAGATCTGCGACTCCCGGAAATTATGCTCGACGTGATAATGCGCAAGCGGGGCCTCGTGCTGTTCGTGGGTGGCACTG
GCTCGGGCAAATCAACGTCCCTTGCTGCCTTGATTGATTACCGCAACAGCAATTCCAACGGCCACATCGTGACTGTGGAA
GACCCTGTTGAATATGTACACCGCCACAAGAAGTGCATCGTTAATCAGCGCGAAGTGGGGGTCGATACTCGAAGCTGGCA
TAACGCACTAAAAAATACATTGCGACAGGCGCCTGATGTGATTTTGATTGGTGAGATCCGCGATCGTGAAACCATGGAAC
ACGCCTTGGCTTTCGCGGAGACTGGACATTTGTGTTTATCGACCTTGCACGCGAACAACGCCAACCAAGCCTTAGACCGT
ATTGTTAACTTCTTTCCAGAAGAGCGGCGTCCTCAGTTAATGATGGATTTATCCCAGAATCTTCAGGCGTTTGTGTCTCA
GCGTTTAATACCCACCGTCGACAACAAACGTGTCGCGGCTATCGAGGTCCTGTTGGGTACGCCATTGGTGTCGGATATTA
TTTTGCGCGGCCAGTTTGACGGTTTGAAAGAGGTGATGGAAAAGTCTGAAAATCTGGGCATGAAAACTTTCGACCAGGCC
TGCTTTGAGCTCTATCAAGAGGGACTTATTAGCGAAGAAGAGGCTTTACGTAATGCTGACTCGGCCAACAACGTTCGTTT
GAAAATCAAGTTCGCTGGCAGTACGGTCGGTGGTGATTTTGAAGACGAAGACGATTTGGGTCTGTCGCTCGACGGTGAAG
ACTTTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-270)

3D structure

• AlphaFold DB: F3L4U6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	53.369	98.933	0.528
pilU	Acinetobacter baylyi ADP1	50.716	93.067	0.472
pilU	Vibrio cholerae strain A1552	50	92.8	0.464
pilT	Pseudomonas aeruginosa PAK	41.349	90.933	0.376
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.598	88.267	0.376
pilT	Legionella pneumophila strain Lp02	42.169	88.533	0.373
pilT	Legionella pneumophila strain ERS1305867	42.169	88.533	0.373
pilT	Pseudomonas stutzeri DSM 10701	41.056	90.933	0.373