Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EY643_RS04690 Genome accession   NZ_CP036422
Coordinates   1040003..1041718 (-) Length   571 a.a.
NCBI ID   WP_152661102.1    Uniprot ID   A0A5P9NHI5
Organism   Halioglobus maricola strain IMCC14385     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1035003..1046718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EY643_RS04665 (EY643_04655) argJ 1035612..1036838 (+) 1227 WP_152661097.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  EY643_RS04670 (EY643_04660) mutT 1036839..1037234 (+) 396 WP_152661098.1 8-oxo-dGTP diphosphatase MutT -
  EY643_RS04675 (EY643_04665) coaE 1037247..1037849 (-) 603 WP_152661099.1 dephospho-CoA kinase -
  EY643_RS04680 (EY643_04670) pilD 1037846..1038709 (-) 864 WP_152661100.1 prepilin peptidase Machinery gene
  EY643_RS04685 (EY643_04675) pilC 1038755..1039972 (-) 1218 WP_152661101.1 type II secretion system F family protein Machinery gene
  EY643_RS04690 (EY643_04680) pilB 1040003..1041718 (-) 1716 WP_152661102.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EY643_RS04695 (EY643_04685) - 1042146..1042631 (+) 486 WP_152661103.1 pilin -
  EY643_RS04700 (EY643_04690) - 1042740..1044686 (+) 1947 WP_152661104.1 hypothetical protein -
  EY643_RS04705 (EY643_04695) - 1044688..1046568 (+) 1881 WP_152661105.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62695.24 Da        Isoelectric Point: 4.6621

>NTDB_id=348819 EY643_RS04690 WP_152661102.1 1040003..1041718(-) (pilB) [Halioglobus maricola strain IMCC14385]
MNDKAIGQLSGLAGRLVAEGMISAEAAAEAQRSASMEQMHFVQFLVEKQNVDGHRLAEVASQEFGVPQFDTECFDTANIP
VGLVDVELVTKHHALPLYRRGNRLFIAVSDPTNLAALDEIKFHTGVNTDAVLLEEQTLSKMISSWVEMQDSLSDGLDDLD
SADLEGIDVSTGESSDDDEGSDLDETPIVRFVNKVLIDAIKQGASDIHFEPYENSYRVRFRTDGVLREMVKPPRNLSHRL
AARLKVMSQMDISERRVPQDGRIQMKLSRNRAIDFRVNTLPTLFGEKIVLRILDPTSAQLGIDALGYEDEQKQMYLKALN
QPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVHVNPKVGLNFAEALRSFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETVTRMLNMGVPAFNVATSVDLIIAQRLARRLCKECAEPADDVPHDVLIK
QGFTDAMLSGATIKKAVGCSICQDGYKGRVGVYEVVRITPAIARIIMEEGNSLQIQDQASEEGFNTLRVSALRKVAQGFI
SLEEANRITVD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=348819 EY643_RS04690 WP_152661102.1 1040003..1041718(-) (pilB) [Halioglobus maricola strain IMCC14385]
ATGAACGACAAAGCCATCGGGCAACTGAGTGGCCTTGCGGGCCGGCTTGTCGCCGAGGGGATGATATCCGCCGAGGCAGC
GGCAGAGGCCCAGCGCAGTGCATCTATGGAACAGATGCACTTTGTGCAATTCCTGGTCGAAAAACAAAATGTAGATGGGC
ACCGCCTCGCTGAGGTGGCATCCCAGGAATTCGGTGTCCCACAGTTTGACACCGAGTGCTTCGACACAGCCAACATTCCG
GTTGGCCTGGTAGATGTCGAGCTCGTCACCAAACACCACGCCCTGCCACTTTATCGCCGCGGCAACCGCCTGTTCATTGC
TGTCTCCGACCCGACCAACCTGGCCGCGCTGGACGAAATCAAATTCCACACCGGGGTCAACACCGACGCGGTCCTGCTGG
AGGAGCAAACCCTCAGCAAGATGATCTCTAGCTGGGTCGAAATGCAGGACTCCCTGTCCGATGGCCTGGACGATTTGGAT
TCCGCCGATCTTGAGGGTATCGACGTCTCAACCGGCGAGAGCAGTGATGACGACGAAGGCAGTGATCTCGATGAAACCCC
CATCGTACGTTTCGTCAACAAGGTTCTGATCGACGCCATCAAACAGGGCGCTTCGGATATTCACTTCGAGCCCTACGAGA
ACAGCTACAGAGTGCGTTTTCGCACCGACGGCGTATTGCGCGAGATGGTCAAGCCACCACGCAACCTGTCGCACCGCCTC
GCAGCGCGCCTCAAAGTCATGTCGCAGATGGACATTTCCGAGCGGCGAGTGCCCCAGGACGGGCGCATCCAGATGAAACT
GTCGCGCAATCGGGCCATCGACTTCCGTGTTAACACCCTGCCGACGCTGTTCGGTGAAAAGATCGTACTGCGTATCCTCG
ACCCCACCAGCGCCCAGTTGGGTATTGATGCGCTTGGCTATGAGGACGAACAGAAACAGATGTATCTGAAGGCCTTGAAT
CAGCCTCAGGGCATGATTCTGGTCACAGGCCCCACTGGTTCGGGTAAAACCGTATCGCTGTATACCGGCCTCAATATTCT
GAACACCGCAGAGCGCAATATTTCCACCGCGGAAGATCCCGTGGAGATCAACCTCGAGGGTATCAACCAGGTTCATGTAA
ACCCCAAAGTTGGCCTTAATTTCGCCGAGGCATTGCGTTCTTTCCTGCGTCAGGATCCGGACATCATCATGGTAGGTGAG
ATTCGCGATCTGGAAACGGCGGAGATAGCCATTAAGGCAGCCCAGACGGGTCACCTGGTACTCTCCACGTTGCACACCAA
TAGCGCGGCCGAGACCGTCACCCGTATGTTGAACATGGGCGTGCCGGCGTTTAACGTCGCGACATCTGTCGATCTGATTA
TTGCCCAGCGACTGGCGCGCCGGCTGTGCAAGGAGTGCGCAGAACCCGCCGATGACGTACCTCATGATGTACTGATCAAA
CAGGGTTTTACCGACGCAATGTTGTCGGGAGCCACCATCAAGAAAGCGGTGGGCTGTAGCATTTGCCAGGACGGCTACAA
AGGCCGTGTCGGCGTATACGAGGTCGTAAGAATCACTCCTGCGATCGCGCGGATCATCATGGAAGAAGGGAATTCACTTC
AAATCCAGGACCAGGCCAGCGAGGAAGGGTTTAATACTCTTCGCGTGTCAGCCCTGCGCAAGGTAGCGCAAGGCTTTATC
AGCCTGGAAGAAGCCAACCGGATCACGGTGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9NHI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.657

99.124

0.581
  pilB Acinetobacter baylyi ADP1

57.979

98.774

0.573
  pilB Legionella pneumophila strain ERS1305867

54.799

100

0.55
  pilB Vibrio cholerae strain A1552

51.852

99.299

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

53.462

91.068

0.487
  pilB Vibrio campbellii strain DS40M4

52.601

90.893

0.478
  pilF Neisseria gonorrhoeae MS11

46.964

98.074

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

41.75

88.091

0.368

Multiple sequence alignment