Detailed information NTG0348310

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: GIX96_RS20540
• Genome accession: NZ_CP045863
• Coordinates: 4097088..4098071 (-)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli O157:H7 strain ATCC 43890
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 4092088..4103071

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GIX96_RS20515 (GIX96_20515)	bcsF	4092249..4092440 (+)	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-
GIX96_RS20520 (GIX96_20520)	bcsG	4092437..4094116 (+)	1680	WP_000191596.1	cellulose biosynthesis protein BcsG	-
GIX96_RS20525 (GIX96_20525)	ldrD	4094203..4094310 (-)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
GIX96_RS20530 (GIX96_20530)	yhjV	4094786..4096057 (+)	1272	WP_001301684.1	aromatic amino acid transport family protein	-
GIX96_RS20535 (GIX96_20535)	dppF	4096087..4097091 (-)	1005	WP_000107027.1	dipeptide ABC transporter ATP-binding subunit DppF	-
GIX96_RS20540 (GIX96_20540)	amiE	4097088..4098071 (-)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
GIX96_RS20545 (GIX96_20545)	dppC	4098082..4098984 (-)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
GIX96_RS20550 (GIX96_20550)	dppB	4098994..4100013 (-)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
GIX96_RS20555 (GIX96_20555)	dppA	4100164..4101771 (-)	1608	WP_001222888.1	dipeptide ABC transporter substrate-binding protein DppA	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=348310 GIX96_RS20540 WP_001196486.1 4097088..4098071(-) (amiE) [Escherichia coli O157:H7 strain ATCC 43890]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=348310 GIX96_RS20540 WP_001196486.1 4097088..4098071(-) (amiE) [Escherichia coli O157:H7 strain ATCC 43890]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCACCGTTCCGCGCCGTAGACCGCATTAG
CTACAGCGTAAAACAGGGCGAAGTAGTCGGGATTGTGGGTGAGTCTGGCTCCGGTAAGTCGGTCAGTTCGCTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTGATGGCGGAAAAGCTGGAGTTTAACGGTCAGGATTTGCAGCGTATCTCGGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGTAATAAGAGCACTCGTCGTCAGCGGGCGATTGACT
TGTTGAATCAGGTCGGTATTCCCGATCCGGCTTCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATTGCGATGGCCATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCAACCACTGCGCTGGACGTGAC
CATTCAGGCGCAAATCATTGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCCGGTCAGGTGGTGGAAACGGGCGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCGGAATTTGCTCAGGACAAAGAACGTCT
GGCATCGTTGCCTGGCGTTGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCTTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404