Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EXN22_RS23425 Genome accession   NZ_CP035952
Coordinates   5129393..5130601 (+) Length   402 a.a.
NCBI ID   WP_130266295.1    Uniprot ID   A0A411MP13
Organism   Pseudomonas tructae strain SNU WT1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5117958..5128527 5129393..5130601 flank 866
IScluster/Tn 5127365..5128527 5129393..5130601 flank 866


Gene organization within MGE regions


Location: 5117958..5130601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EXN22_RS23365 (EXN22_23365) - 5117958..5118185 (+) 228 WP_130266285.1 hypothetical protein -
  EXN22_RS23370 (EXN22_23370) - 5118263..5118559 (+) 297 WP_130266286.1 DUF3077 domain-containing protein -
  EXN22_RS23375 (EXN22_23375) - 5118746..5119207 (-) 462 WP_130266287.1 NUDIX hydrolase -
  EXN22_RS23380 (EXN22_23380) - 5119285..5119728 (-) 444 WP_130266288.1 HIT family protein -
  EXN22_RS23385 (EXN22_23385) - 5119734..5120063 (-) 330 WP_130266289.1 DHCW motif cupin fold protein -
  EXN22_RS23390 (EXN22_23390) - 5121588..5121872 (+) 285 WP_130266290.1 hypothetical protein -
  EXN22_RS23395 (EXN22_23395) - 5122476..5123120 (-) 645 WP_130266291.1 SLATT domain-containing protein -
  EXN22_RS23400 (EXN22_23400) - 5123330..5124769 (-) 1440 WP_165392257.1 reverse transcriptase domain-containing protein -
  EXN22_RS23405 (EXN22_23405) - 5125799..5126974 (+) 1176 WP_233281664.1 reverse transcriptase domain-containing protein -
  EXN22_RS23410 (EXN22_23410) - 5127365..5128527 (+) 1163 WP_130266293.1 IS3 family transposase -
  EXN22_RS23420 (EXN22_23420) - 5128764..5129174 (-) 411 WP_130266294.1 pilin -
  EXN22_RS23425 (EXN22_23425) pilC 5129393..5130601 (+) 1209 WP_130266295.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43310.96 Da        Isoelectric Point: 10.3887

>NTDB_id=345244 EXN22_RS23425 WP_130266295.1 5129393..5130601(+) (pilC) [Pseudomonas tructae strain SNU WT1]
MTASNRMYHWQGTNAQGALVNGQSAARSPALIRAQLRQRGIRPGRIDSVRAPLWQWRKPLDGKALSQFSRQLATLVKAGV
ALLQALEVVAQSSANPAMASLLGAIKADIAAGSSLANALRRHPQYFDRLYCNLVDAGEQSGSLETVLAQVAALQEQRQAL
RARLKKAMTYPLLVLLVGFGVSALLLLEVVPRFQSLFAGFGSELPVFTQQVISLSQWLGAQLGWLLISLCCVTIAVLQAY
RRKPGFRLWLMRLALRLPVLGSLLQHAALARFARTLSTSFAAGVPLVEALGPVAGACGNPLFEQALHKVREDLASGQALH
VALRASGLFPTLVVQMCAIGETAGTLDDMLARIASHYEQAIDHLLDNLTALLEPLIVLILGLLVGSLVIAMYLPIFQLGN
VI

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=345244 EXN22_RS23425 WP_130266295.1 5129393..5130601(+) (pilC) [Pseudomonas tructae strain SNU WT1]
ATGACCGCAAGCAACCGTATGTACCACTGGCAAGGCACCAACGCCCAGGGCGCCTTGGTCAATGGCCAGAGCGCCGCGCG
CAGCCCGGCACTGATCAGGGCGCAGTTGCGCCAGCGCGGGATTCGCCCGGGGCGCATCGACAGTGTGCGCGCGCCATTGT
GGCAATGGCGCAAGCCACTGGATGGCAAAGCTTTGAGCCAGTTCAGCCGCCAGCTGGCCACCCTGGTCAAGGCCGGGGTA
GCGTTGTTGCAGGCGCTGGAGGTGGTCGCCCAAAGCAGCGCCAACCCGGCGATGGCCAGCCTGCTGGGGGCGATCAAGGC
CGATATCGCCGCCGGCAGCAGCCTGGCCAATGCTTTGCGCCGGCATCCGCAATATTTCGATCGGCTGTACTGCAACCTGG
TGGACGCCGGCGAGCAATCCGGCAGCCTGGAGACCGTCCTGGCCCAGGTGGCGGCACTGCAGGAGCAACGCCAGGCCCTG
CGCGCGCGGCTGAAAAAGGCCATGACGTATCCACTGCTGGTGCTGCTGGTGGGCTTTGGGGTGTCGGCGTTGTTGCTGTT
GGAGGTGGTGCCGCGCTTTCAGTCGTTGTTCGCAGGCTTTGGTTCCGAACTACCGGTCTTCACCCAGCAGGTGATCAGCT
TGTCGCAGTGGTTGGGCGCGCAGCTCGGGTGGCTGTTGATAAGCTTGTGCTGTGTAACCATCGCGGTGCTCCAGGCTTAT
CGGCGCAAGCCCGGGTTTCGCTTGTGGTTGATGCGTTTGGCCCTGCGCCTGCCAGTGCTGGGCAGCCTGCTCCAGCATGC
CGCCCTCGCCCGCTTTGCCCGCACCTTGTCGACCTCGTTTGCCGCCGGAGTGCCGTTGGTTGAAGCGCTGGGGCCGGTTG
CCGGGGCCTGTGGCAATCCGTTGTTCGAGCAGGCGCTGCACAAGGTGCGCGAGGACCTGGCCAGTGGTCAGGCGTTGCAC
GTGGCACTGCGCGCCAGCGGTTTGTTCCCGACGCTGGTGGTGCAGATGTGCGCCATTGGCGAGACAGCCGGCACGCTGGA
CGATATGCTCGCCAGGATCGCCAGCCACTATGAACAGGCTATCGACCACCTGCTCGATAACCTGACCGCCCTGCTCGAAC
CCTTGATCGTGCTGATCCTCGGGCTGCTGGTGGGCAGCCTGGTGATCGCCATGTACCTGCCAATCTTCCAGCTGGGCAAT
GTGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411MP13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

49.497

99.005

0.49
  pilC Acinetobacter baylyi ADP1

45.885

99.751

0.458
  pilC Acinetobacter baumannii D1279779

45.34

98.756

0.448
  pilC Legionella pneumophila strain ERS1305867

45.226

99.005

0.448
  pilC Vibrio cholerae strain A1552

41.103

99.254

0.408
  pilG Neisseria gonorrhoeae MS11

40.256

97.015

0.391
  pilG Neisseria meningitidis 44/76-A

40.256

97.015

0.391
  pilC Vibrio campbellii strain DS40M4

38.945

99.005

0.386
  pilC Thermus thermophilus HB27

36.658

99.751

0.366

Multiple sequence alignment