Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EPB57_RS20860 Genome accession   NZ_CP035700
Coordinates   2655088..2656311 (-) Length   407 a.a.
NCBI ID   WP_154205049.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2013V-1302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2650088..2661311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB57_RS20830 trmD 2650607..2651368 (+) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EPB57_RS20835 rplS 2651410..2651763 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  EPB57_RS20840 yacG 2652418..2652612 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  EPB57_RS20845 zapD 2652771..2653511 (-) 741 WP_005373086.1 cell division protein ZapD -
  EPB57_RS20850 coaE 2653539..2654153 (-) 615 WP_137282357.1 dephospho-CoA kinase -
  EPB57_RS20855 pilD 2654154..2655023 (-) 870 WP_154205048.1 A24 family peptidase Machinery gene
  EPB57_RS20860 pilC 2655088..2656311 (-) 1224 WP_154205049.1 type II secretion system F family protein Machinery gene
  EPB57_RS20865 pilB 2656335..2658020 (-) 1686 WP_154205050.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPB57_RS20870 - 2658032..2658490 (-) 459 WP_154205051.1 pilin -
  EPB57_RS20875 nadC 2658746..2659633 (-) 888 WP_047102656.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EPB57_RS20880 ampD 2659726..2660277 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45289.39 Da        Isoelectric Point: 10.3992

>NTDB_id=343936 EPB57_RS20860 WP_154205049.1 2655088..2656311(-) (pilC) [Vibrio alginolyticus strain 2013V-1302]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFTSA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLATEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=343936 EPB57_RS20860 WP_154205049.1 2655088..2656311(-) (pilC) [Vibrio alginolyticus strain 2013V-1302]
ATGAAAAGTACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACGGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TTTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATCACGGTGTTTACCCGCCAAATTTCGACGATGTTG
ATAACCGGTGTGCCCTTGGTTCAGGCTTTAAAATTGGTATCAGAGAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACTGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTCGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACTAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCACCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACCTACACTATCAACTCGCCACAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTAGCCATGCGCAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATCGGTGAAGAGTC
TGGTCGACTTGACGACATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGATAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTTTTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.732

100

0.867
  pilC Vibrio cholerae strain A1552

73.529

100

0.737
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Legionella pneumophila strain ERS1305867

40.976

100

0.413
  pilC Acinetobacter baumannii D1279779

41.75

98.28

0.41
  pilG Neisseria gonorrhoeae MS11

40.741

99.509

0.405
  pilC Pseudomonas stutzeri DSM 10701

41.162

97.297

0.4
  pilG Neisseria meningitidis 44/76-A

40.148

99.754

0.4

Multiple sequence alignment