Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EPB61_RS11170 Genome accession   NZ_CP035696
Coordinates   787537..789225 (+) Length   562 a.a.
NCBI ID   WP_154180386.1    Uniprot ID   -
Organism   Vibrio furnissii strain 2419-04     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782537..794225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPB61_RS11140 dsbC 782872..783636 (-) 765 WP_055466521.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  EPB61_RS11145 xerD 783653..784507 (-) 855 WP_231623439.1 site-specific tyrosine recombinase XerD -
  EPB61_RS11150 fldB 784702..785223 (+) 522 WP_004729075.1 flavodoxin FldB -
  EPB61_RS11155 ampD 785245..785796 (-) 552 WP_154180384.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EPB61_RS11160 nadC 785947..786834 (+) 888 WP_154180385.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EPB61_RS11165 - 787081..787530 (+) 450 WP_055466517.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EPB61_RS11170 pilB 787537..789225 (+) 1689 WP_154180386.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EPB61_RS11175 pilC 789304..790530 (+) 1227 WP_154180387.1 type II secretion system F family protein Machinery gene
  EPB61_RS11180 pilD 790582..791451 (+) 870 WP_154180388.1 A24 family peptidase Machinery gene
  EPB61_RS11185 coaE 791454..792062 (+) 609 WP_154180389.1 dephospho-CoA kinase -
  EPB61_RS11190 zapD 792095..792835 (+) 741 WP_154180390.1 cell division protein ZapD -
  EPB61_RS11195 yacG 792893..793090 (+) 198 WP_004729092.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62028.17 Da        Isoelectric Point: 6.1994

>NTDB_id=343814 EPB61_RS11170 WP_154180386.1 787537..789225(+) (pilB) [Vibrio furnissii strain 2419-04]
MLTNLIAILRQAKLIGPTQEQAVIDHIHASGATVPEALIDLAIFHHHDLTEQLSQIFGLPETDLTRFDYAPLCQQLGLRE
LITRYQALPLHSNGQILTLAVADPTHLQVEDEFRFATGLQVELVLADHKALHAAIRRLYGRSLATPGTQGKEISQDELAD
LVQISDDEKTTIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESHYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLDADTAIDMRVSTLPTLWGEKIVLRLLDSSAANLNIDKLGYNEQQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTEQVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVAITPPAALQQQFGLLAED
AVYEAHPSGCTECTNGYSGRVGIYEVMAFHPALADAVMQRASIHQIETIAQQHGMQPLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=343814 EPB61_RS11170 WP_154180386.1 787537..789225(+) (pilB) [Vibrio furnissii strain 2419-04]
ATGCTGACCAACCTTATCGCCATTCTGCGTCAGGCTAAGTTGATCGGCCCAACGCAGGAACAGGCCGTGATCGACCATAT
TCACGCTTCCGGTGCCACGGTGCCGGAGGCGTTGATCGATTTGGCGATATTTCATCATCACGATTTAACCGAGCAACTAA
GTCAGATCTTTGGCCTACCCGAAACCGATCTCACTCGGTTTGATTACGCGCCATTGTGCCAGCAGTTGGGACTTCGAGAG
CTCATTACCCGCTACCAAGCCCTGCCGCTGCACAGCAATGGCCAAATCCTGACGTTGGCGGTTGCTGACCCAACTCACCT
GCAAGTCGAAGACGAGTTTCGCTTTGCCACCGGATTACAGGTGGAACTGGTGCTCGCCGATCATAAAGCGCTGCACGCCG
CCATTCGCCGCTTGTATGGGCGCAGCCTCGCGACGCCGGGGACGCAAGGCAAAGAGATCAGTCAGGATGAATTGGCGGAT
CTGGTACAGATCTCTGACGACGAAAAAACCACCATTGAAGATCTCAGCCAAGATGATTCACCGGTCAGTCGTTTTATCAA
CCAGATCCTACTCGATGCCGTGCGCAAAGGTGCGTCCGACATTCACTTTGAGCCGTATGAGTCCCACTACCGTATTCGTC
TGCGCTGTGACGGCATTCTGGTCGAAACCCAACAACCTGCCAGTCACCTCAGCCGACGCTTGGCAGCCCGAATCAAAATT
TTGTCGAAACTGGATATTGCCGAGCGCCGCCTCCCGCAAGATGGACGCATCAAACTGCGCTTGGATGCCGACACCGCGAT
TGATATGCGTGTATCGACCTTGCCAACATTGTGGGGTGAAAAGATTGTTCTGCGCTTACTCGATAGCAGCGCGGCCAACC
TCAACATCGACAAACTCGGCTACAACGAACAGCAGAAACAGCTCTACCTCGCAGCCCTCACCAAACCGCAAGGCATGATC
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTGTCGCTCTACACCGGATTACGCATTCTCAATACCGAACAAGTGAA
CATCTCCACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGTATCAATCAGGTGCAAGTTCAACCCAAAATCGGCTTTG
GCTTTGCACAAGCGCTGCGCTCGTTTCTACGCCAAGATCCCGATGTGGTGATGGTGGGCGAAATTCGCGATCTAGAAACC
GCAGAAATCGCCATTAAAGCCGCGCAAACCGGGCACTTGGTGCTCTCAACCTTGCACACCAACTCGGCCGCGGAAACCGT
GATTCGTTTAGCCAACATGGGCATTGAACCCTTTAACCTTGCCTCATCATTGAGCCTGATCATTGCGCAGCGGCTGGCCC
GCCGTCTGTGCCCGCATTGCAAGGTCGCCATCACGCCCCCAGCTGCGCTGCAGCAACAATTTGGCTTGCTCGCAGAAGAT
GCTGTATACGAAGCACATCCATCGGGTTGCACGGAATGCACCAATGGGTATAGCGGCCGCGTGGGCATTTATGAAGTGAT
GGCGTTTCATCCTGCCTTGGCGGACGCGGTGATGCAGCGTGCCAGCATTCATCAAATCGAAACCATTGCCCAACAGCACG
GCATGCAACCCTTGCTGCAATCTGGGATTGAAAAACTGCGCGCGGGCATCACCAGTTTTAGTGAATTACAGCGAGTGCTC
TACTTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.206

100

0.822
  pilB Vibrio campbellii strain DS40M4

71.886

100

0.719
  pilB Vibrio parahaemolyticus RIMD 2210633

71.886

100

0.719
  pilB Legionella pneumophila strain ERS1305867

52.519

91.815

0.482
  pilB Acinetobacter baumannii D1279779

51.06

92.349

0.472
  pilB Acinetobacter baylyi ADP1

50.097

91.993

0.461
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.636

97.865

0.368
  pilF Thermus thermophilus HB27

36.655

100

0.367

Multiple sequence alignment