Detailed information NTG0033856

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MDS_RS24365
• Genome accession: NC_015410
• Coordinates: 5241236..5242366 (+)
• Length: 376 a.a.
• NCBI ID: WP_013717824.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina NK-01
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5236236..5247366

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MDS_RS24340 (MDS_4782)	-	5236577..5236897 (+)	321	WP_013717819.1	DUF2388 domain-containing protein	-
MDS_RS24345 (MDS_4783)	-	5236894..5238849 (+)	1956	WP_013717820.1	DUF4105 domain-containing protein	-
MDS_RS24350 (MDS_4784)	-	5238914..5239318 (-)	405	WP_013717821.1	GFA family protein	-
MDS_RS24355 (MDS_4785)	-	5239387..5240292 (+)	906	WP_013717822.1	AEC family transporter	-
MDS_RS24360 (MDS_4786)	-	5240308..5241045 (-)	738	WP_237705680.1	ABC transporter substrate-binding protein	-
MDS_RS24365 (MDS_4787)	pilU	5241236..5242366 (+)	1131	WP_013717824.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MDS_RS24370 (MDS_4788)	-	5242454..5244094 (+)	1641	WP_013717825.1	Na/Pi cotransporter family protein	-
MDS_RS24375 (MDS_4789)	-	5244096..5245604 (-)	1509	WP_013717826.1	uracil permease	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 41842.84 Da
• Isoelectric Point: 6.1930

>NTDB_id=33856 MDS_RS24365 WP_013717824.1 5241236..5242366(+) (pilU) [Ectopseudomonas mendocina NK-01]
MNDTSANDQPDVFPYLQLMHQHAGSDLFFSVGAPPHMKVEGHSQPVGQRIMKAGEVQQLAYQLMTQKQIAEFERDLEMNL
AVSLQGAGRYRVNVYYQRGEVAMVVRLIKSEIPGFEALGLPKLLEKLAMQDRGLILVTGAAGSGKSTTLAAMLDFRNRHK
SGHIVCIEDPIEFLHSHQRSIIDQREVGLDTHSFDDALRNVLREAPDVIMLGEIRDAATMQHALHYAETGHLCVATLHAT
SSSHAIERIARFFPDDARKQVLADVAHNLLAVIGQRLVPGIEQKRVAAIELMLGTPYIRDLVQRDELDELREAIARAAEQ
GLQTFDQHLFALLEAGRISLAEALKFADSRTDLSLKFKLERGFSADDAELKVLRDG

Nucleotide

• Length: 1131 bp

>NTDB_id=33856 MDS_RS24365 WP_013717824.1 5241236..5242366(+) (pilU) [Ectopseudomonas mendocina NK-01]
ATGAACGACACCAGCGCCAACGACCAGCCCGATGTTTTCCCCTACCTGCAGCTGATGCATCAACATGCCGGCTCGGACCT
GTTCTTCAGCGTCGGCGCGCCGCCGCACATGAAGGTCGAAGGGCATAGCCAGCCCGTCGGCCAGCGCATCATGAAGGCCG
GCGAGGTGCAGCAGCTGGCCTATCAACTGATGACGCAGAAACAGATCGCCGAGTTCGAACGCGACCTGGAGATGAACCTG
GCCGTCAGCCTGCAGGGCGCCGGACGCTATCGGGTCAACGTCTATTACCAGCGCGGCGAAGTGGCCATGGTGGTGCGCCT
GATCAAGAGCGAGATCCCTGGTTTCGAGGCGCTCGGTCTACCCAAGCTGCTGGAGAAACTGGCCATGCAGGACCGCGGGC
TGATCCTGGTCACCGGCGCAGCCGGCTCGGGCAAGTCCACCACCCTGGCGGCGATGCTGGATTTTCGCAATCGCCACAAG
AGCGGGCATATCGTCTGCATCGAAGACCCCATCGAGTTTCTCCACAGCCATCAGCGCTCGATCATCGACCAGCGTGAAGT
CGGCCTCGATACCCACAGCTTCGATGACGCCCTGCGCAACGTGCTGCGTGAGGCGCCGGACGTGATCATGCTCGGCGAAA
TCCGCGACGCCGCCACCATGCAGCACGCTCTGCACTACGCCGAAACAGGCCATTTGTGCGTCGCCACTCTGCATGCCACC
AGCAGCAGCCACGCCATCGAACGCATCGCCCGCTTCTTCCCCGACGACGCTCGCAAACAGGTACTGGCCGACGTGGCGCA
CAACCTGCTCGCGGTGATCGGCCAACGCCTGGTGCCTGGCATCGAGCAGAAGCGCGTGGCGGCGATTGAGCTGATGCTCG
GCACGCCCTATATCCGCGATCTGGTCCAGCGCGACGAACTGGATGAACTGCGCGAGGCCATCGCACGAGCCGCCGAGCAG
GGTTTGCAGACCTTCGACCAGCATCTGTTCGCTCTGCTCGAGGCCGGACGCATCAGCCTGGCAGAAGCGCTCAAGTTTGC
CGACTCGCGTACCGACCTCAGCCTCAAGTTCAAGCTCGAGCGCGGTTTCTCCGCCGACGACGCCGAACTCAAGGTGCTGC
GCGACGGTTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (52-278)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	47.514	96.277	0.457
pilU	Vibrio cholerae strain A1552	47.278	92.819	0.439
pilU	Acinetobacter baylyi ADP1	46.154	93.351	0.431
pilT	Acinetobacter baylyi ADP1	41.888	90.16	0.378
pilT	Vibrio cholerae strain A1552	40.751	92.021	0.375
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.751	92.021	0.375
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.828	89.894	0.367
pilT	Pseudomonas stutzeri DSM 10701	41.641	87.5	0.364