Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EQH19_RS09660 Genome accession   NZ_CP035261
Coordinates   1879935..1880951 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901924     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1874935..1885951
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH19_RS10795 - 1875012..1875143 (-) 132 WP_000768904.1 hypothetical protein -
  EQH19_RS09620 (EQH19_10160) - 1875144..1876334 (-) 1191 WP_000167757.1 acetate kinase -
  EQH19_RS09625 (EQH19_10165) comYH 1876385..1877338 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  EQH19_RS09630 (EQH19_10170) - 1877399..1877993 (-) 595 Protein_1891 class I SAM-dependent methyltransferase -
  EQH19_RS09635 (EQH19_10175) comGG/cglG 1878130..1878543 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH19_RS09640 (EQH19_10180) comGF/cglF 1878521..1878982 (-) 462 WP_000250532.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH19_RS09645 (EQH19_10185) comGE/cglE 1878945..1879247 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQH19_RS09650 (EQH19_10190) comGD/cglD 1879210..1879614 (-) 405 WP_000588029.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH19_RS09655 (EQH19_10195) comGC/cglC 1879607..1879933 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  EQH19_RS09660 (EQH19_10200) comGB/cglB 1879935..1880951 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH19_RS09665 (EQH19_10205) comGA/cglA/cilD 1880899..1881840 (-) 942 WP_000249555.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH19_RS09670 (EQH19_10210) - 1881915..1882280 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH19_RS09675 (EQH19_10215) - 1882431..1883489 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH19_RS09680 (EQH19_10220) nagA 1883652..1884803 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=338418 EQH19_RS09660 WP_074017570.1 1879935..1880951(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901924]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=338418 EQH19_RS09660 WP_074017570.1 1879935..1880951(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901924]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment