Detailed information NTG0338377

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: EQH19_RS04525
• Genome accession: NZ_CP035261
• Coordinates: 899489..900139 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387332.1
• Uniprot ID: A0A6I3U9C7
• Organism: Streptococcus pneumoniae strain TVO_1901924
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 894489..905139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH19_RS04495 (EQH19_04770)	-	894972..895187 (+)	216	WP_001232082.1	YozE family protein	-
EQH19_RS04500 (EQH19_04775)	-	895273..896241 (+)	969	WP_000658183.1	PhoH family protein	-
EQH19_RS04505 (EQH19_04785)	-	896434..896934 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
EQH19_RS04510 (EQH19_04790)	-	896937..897263 (+)	327	Protein_900	TfoX/Sxy family protein	-
EQH19_RS04515	ald	897564..898675 (-)	1112	Protein_901	alanine dehydrogenase	-
EQH19_RS04520 (EQH19_04810)	-	898852..899421 (+)	570	WP_000443775.1	GNAT family N-acetyltransferase	-
EQH19_RS04525 (EQH19_04815)	comEA/celA/cilE	899489..900139 (+)	651	WP_000387332.1	helix-hairpin-helix domain-containing protein	Machinery gene
EQH19_RS04530 (EQH19_04820)	comEC/celB	900123..902363 (+)	2241	WP_000942425.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH19_RS10840 (EQH19_04825)	-	902512..902729 (+)	218	Protein_905	hypothetical protein	-
EQH19_RS04535 (EQH19_04830)	-	902762..903349 (+)	588	WP_000939880.1	ATP-binding cassette domain-containing protein	-
EQH19_RS04540 (EQH19_04835)	-	903353..904534 (+)	1182	WP_000655935.1	membrane protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23219.57 Da
• Isoelectric Point: 5.5171

>NTDB_id=338377 EQH19_RS04525 WP_000387332.1 899489..900139(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901924]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSIDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=338377 EQH19_RS04525 WP_000387332.1 899489..900139(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901924]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTAAAGGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAGCTTAAACAGGTTAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATAGACCATCGTGAGGCAAATGG
GAAATTCAAGTCGATTGATGAACTCAAGAAGGTCTCTGGCATTGGTGCTAAGACCATAGAAAAGCTAAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A6I3U9C7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae D39	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae R6	98.148	100	0.981
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.222	100	0.972
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis SK321	91.204	100	0.912
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37
comEA	Bacillus subtilis subsp. subtilis str. 168	42.105	87.963	0.37