Detailed information NTG0338299

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: EQH20_RS04180
• Genome accession: NZ_CP035260
• Coordinates: 844604..845254 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387331.1
• Uniprot ID: A0A0E7VQD3
• Organism: Streptococcus pneumoniae strain TVO_1901925
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 839604..850254

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH20_RS04150 (EQH20_04375)	-	840088..840303 (+)	216	WP_001232082.1	YozE family protein	-
EQH20_RS04155 (EQH20_04380)	-	840389..841357 (+)	969	WP_000658183.1	PhoH family protein	-
EQH20_RS04160 (EQH20_04390)	-	841550..842050 (+)	501	WP_000566982.1	GNAT family N-acetyltransferase	-
EQH20_RS04165 (EQH20_04395)	-	842053..842378 (+)	326	Protein_830	TfoX/Sxy family protein	-
EQH20_RS04170	ald	842679..843790 (-)	1112	Protein_831	alanine dehydrogenase	-
EQH20_RS04175 (EQH20_04415)	-	843967..844536 (+)	570	WP_000443774.1	GNAT family N-acetyltransferase	-
EQH20_RS04180 (EQH20_04420)	comEA/celA/cilE	844604..845254 (+)	651	WP_000387331.1	ComEA family DNA-binding protein	Machinery gene
EQH20_RS04185 (EQH20_04425)	comEC/celB	845238..847478 (+)	2241	WP_000942403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH20_RS04190 (EQH20_04430)	-	847657..847845 (+)	189	WP_001809102.1	hypothetical protein	-
EQH20_RS04195 (EQH20_04435)	-	847877..848464 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
EQH20_RS04200 (EQH20_04440)	-	848468..849649 (+)	1182	WP_061647495.1	hypothetical protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23203.57 Da
• Isoelectric Point: 5.5171

>NTDB_id=338299 EQH20_RS04180 WP_000387331.1 844604..845254(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901925]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=338299 EQH20_RS04180 WP_000387331.1 844604..845254(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901925]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGCGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACACCTGTAAAGGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

3D structure

• AlphaFold DB: A0A0E7VQD3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae D39	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae R6	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.685	100	0.977
comEA/celA/cilE	Streptococcus mitis NCTC 12261	96.296	100	0.963
comEA/celA/cilE	Streptococcus mitis SK321	90.741	100	0.907
comEA	Lactococcus lactis subsp. cremoris KW2	43.612	100	0.458
comEA	Bacillus subtilis subsp. subtilis str. 168	41.579	87.963	0.366
comEA	Latilactobacillus sakei subsp. sakei 23K	33.476	100	0.361