Detailed information NTG0338300

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: EQH20_RS04185
• Genome accession: NZ_CP035260
• Coordinates: 845238..847478 (+)
• Length: 746 a.a.
• NCBI ID: WP_000942403.1
• Uniprot ID: B1IBB4
• Organism: Streptococcus pneumoniae strain TVO_1901925
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 840238..852478

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH20_RS04155 (EQH20_04380)	-	840389..841357 (+)	969	WP_000658183.1	PhoH family protein	-
EQH20_RS04160 (EQH20_04390)	-	841550..842050 (+)	501	WP_000566982.1	GNAT family N-acetyltransferase	-
EQH20_RS04165 (EQH20_04395)	-	842053..842378 (+)	326	Protein_830	TfoX/Sxy family protein	-
EQH20_RS04170	ald	842679..843790 (-)	1112	Protein_831	alanine dehydrogenase	-
EQH20_RS04175 (EQH20_04415)	-	843967..844536 (+)	570	WP_000443774.1	GNAT family N-acetyltransferase	-
EQH20_RS04180 (EQH20_04420)	comEA/celA/cilE	844604..845254 (+)	651	WP_000387331.1	ComEA family DNA-binding protein	Machinery gene
EQH20_RS04185 (EQH20_04425)	comEC/celB	845238..847478 (+)	2241	WP_000942403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH20_RS04190 (EQH20_04430)	-	847657..847845 (+)	189	WP_001809102.1	hypothetical protein	-
EQH20_RS04195 (EQH20_04435)	-	847877..848464 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
EQH20_RS04200 (EQH20_04440)	-	848468..849649 (+)	1182	WP_061647495.1	hypothetical protein	-
EQH20_RS04205 (EQH20_04445)	infC	849956..850486 (+)	531	WP_061647496.1	translation initiation factor IF-3	-
EQH20_RS04210 (EQH20_04450)	rpmI	850519..850719 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
EQH20_RS04215 (EQH20_04455)	rplT	850771..851130 (+)	360	WP_000124836.1	50S ribosomal protein L20	-
EQH20_RS04220 (EQH20_04460)	-	851188..851568 (+)	381	WP_000157154.1	VOC family protein	-

Sequence

Protein

• Length: 746 a.a.
• Molecular weight: 84530.99 Da
• Isoelectric Point: 9.5181

>NTDB_id=338300 EQH20_RS04185 WP_000942403.1 845238..847478(+) (comEC/celB) [Streptococcus pneumoniae strain TVO_1901925]
MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCLFIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQN
LADSVERVRILPDTIKVNGDSLSFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLLGHLDTDFEEMNELYSSLGIIHL
FALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPFSLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIV
MPNFFLTAGGVLSCAYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDLTFLPLLSI
LFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLISLALVYDLRKNIKKLTVLCLLITGLFLLTK
HPLENEITMLDVGQGESIFLRDVTGKTILIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDK
ENVGDLSEVTKAFHVGEILVSKDSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRKMGDGGHDDTLVLYGKF
LDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSSSPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGIN
SKVYRTDQQGAIRFKGLDSWKIESVR

Nucleotide

• Length: 2241 bp

>NTDB_id=338300 EQH20_RS04185 WP_000942403.1 845238..847478(+) (comEC/celB) [Streptococcus pneumoniae strain TVO_1901925]
ATGTTACAGTGGATTAAGAATTTCTCTATTCCCCTAATTTACCTGAGTTTTCTATTACTTTGGCTTTATTACGCTATTTT
CTCAGCATCTTATCTTGCTTTGTTGGGCTTTGTTTTTCTGCTAGTCTGTCTCTTTATCCAATTTCCGTGGAAATCTGCTG
GTAAAGTTCTAATAATTTGCGGAATCTTTGGATTTTGGTTTGTTTTTCAAAATTGGCAACAGAGTCAAGCGAGTCAAAAT
CTGGCGGATTCTGTTGAAAGGGTACGGATTTTGCCTGATACTATTAAGGTTAATGGTGATAGTCTATCCTTTCGTGGCAA
GTCTAACGGTCGTGCTTTCCAAGTCTATTATAAACTCCAGTCCGAGGAGGAGAAAGAAGCCTTTCAAGCTTTAACCGACC
TGCATGAGATAGGACTAGAAGGGAAGCTTTCGGAGCCAGAAGGGCAGAGAAATTTTGGTGGCTTTAATTACCAAGCCTAT
CTGAAGACTCAGGGAATTTACCAGACTCTCAATATCAAAACAATCCAGTCACTTCAAAAGATTGGCAGTTGGGATATAGG
AGAAAACTTGTCCAGTTTACGTCGAAAGGCTGTGGTTTGGATTAAGACGCACTTTCCAGACCCTATGCGCAATTACATGA
CAGGACTCTTGCTGGGACATCTGGACACCGACTTTGAGGAGATGAATGAGCTTTATTCCAGTCTAGGAATTATCCACCTC
TTTGCCCTATCTGGTATGCAGGTAGGTTTTTTCATGAATGGATTTAAGAAACTTCTCTTGCGATTGGGCTTGACCCAAGA
AAAGTTGAAATGGCTGACTTATCCCTTTTCCCTTATCTATGCGGGACTAACTGGATTTTCAGCATCGGTTATTCGCAGTC
TCTTGCAAAAGCTACTGGCTCAACATGGGGTTAAGGGCTTGGATAATTTTGCCTTGACGGTGCTTGTCCTCTTTATTGTC
ATGCCAAACTTTTTCTTGACAGCAGGAGGAGTCTTGTCCTGCGCTTATGCTTTTATCCTGACCATGACCAGCAAAGAAGG
GGAGGGGCTCAAGGCTGTTACTAGTGAAAGTCTAGTCATCTCCTTGGGCATATTGCCCATTCTATCCTTCTATTTTGCGG
AATTTCAACCTTGGTCTATCCTTTTGACCTTTGTCTTTTCCTTTCTATTTGACTTGACCTTCTTACCGCTCTTGTCTATT
TTATTTGTCCTTTCCTTTCTCTATCCAGTCATTCAGCTGAACTTTATCTTTGAATGGTTGGAGGGCATTATTCGCTTGGT
GTCACAGGTGACAAGTAGACCTCTGGTCTTTGGACAACCCAATGCATGGTTTTTAATCCTATTGTTAATTTCCTTGGCTT
TGGTCTATGATTTGAGAAAAAACATTAAAAAGCTAACGGTATTGTGCTTATTGATTACAGGGCTCTTTCTCCTGACCAAG
CATCCACTGGAAAATGAAATCACCATGCTGGATGTGGGGCAAGGAGAAAGTATTTTCCTACGGGATGTAACTGGGAAAAC
CATTCTCATAGATGTAGGTGGTAAGGCAGAATCTTATAAGAAAATCGAAAAATGGCAAGAAAAGATGACGACCAGCAATG
CCCAGCGAACCTTGATTCCCTATCTCAAAAGTCGAGGAGTAGCTAAGATTGACCAGCTAATTTTGACTAACACGGACAAG
GAGAATGTTGGAGATTTGTCAGAGGTGACCAAGGCTTTCCATGTAGGGGAGATTCTAGTATCAAAAGACAGTCTGAAACA
GAAGGAATTTGTGGCAGAACTACAGACGACTCAAACAAAGGTGCGTAGTATGACAGTAGGGGAGAACTTGCCCATTTTTG
GAAGTCAGTTAGAAGTTCTATCTCCAAGGAAAATGGGAGATGGAGGACACGATGACACCCTAGTTCTGTATGGGAAATTC
TTGGATAAGCAATTTCTCTTCACGGGAAATTTGGAGGAGAAAGGAGAGAAGGACTTGCTGAAGCACTATCCAGACTTGAA
AGTAAATGTTTTGAAAGCTAGCCAACATGGCAATAAAAAATCATCAAGTCCAGCCTTTCTAGAAAAACTCAAACCAGAGC
TTACTCTTATCTCAGTTGGAAAGAGCAATCGAATGAAACTCCCCCATCAGGAAACATTGACACGACTGGAAGGTATCAAT
AGCAAAGTTTATCGAACTGACCAGCAAGGAGCTATACGTTTTAAGGGGTTGGATAGTTGGAAAATCGAAAGTGTTCGATA
G

Domains

Predicted by InterproScan.

• Domain of unknown function (DUF4131) (DUF4131) (30-176)

• Competence protein (Competence) (216-458)

3D structure

• AlphaFold DB: B1IBB4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus pneumoniae TIGR4	98.123	100	0.981
comEC/celB	Streptococcus pneumoniae D39	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae R6	97.855	100	0.979
comEC/celB	Streptococcus pneumoniae Rx1	97.855	100	0.979
comEC/celB	Streptococcus mitis NCTC 12261	91.946	99.866	0.918
comEC/celB	Streptococcus mitis SK321	91.689	100	0.917
comEC	Lactococcus lactis subsp. cremoris KW2	44.13	99.33	0.438