Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   EQH33_RS09350 Genome accession   NZ_CP035247
Coordinates   1839225..1840241 (-) Length   338 a.a.
NCBI ID   WP_076750272.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901940     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1834225..1845241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQH33_RS10455 - 1834302..1834433 (-) 132 WP_000768904.1 hypothetical protein -
  EQH33_RS09310 (EQH33_09860) - 1834434..1835624 (-) 1191 WP_000167766.1 acetate kinase -
  EQH33_RS09315 (EQH33_09865) comYH 1835675..1836628 (-) 954 WP_000345101.1 class I SAM-dependent methyltransferase Machinery gene
  EQH33_RS09320 (EQH33_09870) - 1836689..1837283 (-) 595 Protein_1828 class I SAM-dependent methyltransferase -
  EQH33_RS09325 (EQH33_09875) comGG/cglG 1837420..1837833 (-) 414 WP_000265624.1 competence type IV pilus minor pilin ComGG Machinery gene
  EQH33_RS09330 (EQH33_09880) comGF/cglF 1837811..1838272 (-) 462 WP_000250542.1 competence type IV pilus minor pilin ComGF Machinery gene
  EQH33_RS09335 (EQH33_09885) comGE/cglE 1838235..1838537 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  EQH33_RS09340 (EQH33_09890) comGD/cglD 1838500..1838904 (-) 405 WP_000588013.1 competence type IV pilus minor pilin ComGD Machinery gene
  EQH33_RS09345 (EQH33_09895) comGC/cglC 1838897..1839223 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  EQH33_RS09350 (EQH33_09900) comGB/cglB 1839225..1840241 (-) 1017 WP_076750272.1 competence type IV pilus assembly protein ComGB Machinery gene
  EQH33_RS09355 (EQH33_09905) comGA/cglA/cilD 1840189..1841130 (-) 942 WP_000249556.1 competence type IV pilus ATPase ComGA Machinery gene
  EQH33_RS09360 (EQH33_09910) - 1841206..1841571 (-) 366 WP_000286415.1 DUF1033 family protein -
  EQH33_RS09365 (EQH33_09915) - 1841722..1842780 (-) 1059 WP_000649466.1 zinc-dependent alcohol dehydrogenase family protein -
  EQH33_RS09370 (EQH33_09920) nagA 1842943..1844094 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38609.67 Da        Isoelectric Point: 9.4800

>NTDB_id=337550 EQH33_RS09350 WP_076750272.1 1839225..1840241(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901940]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMIVFVSVGILLALTFYKRSSKMRVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=337550 EQH33_RS09350 WP_076750272.1 1839225..1840241(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901940]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGATTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATCTTT
CTAGGAATGATAGTTTTTGTTTCAGTAGGTATCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCGCGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCTATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae D39

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae R6

97.633

100

0.976
  comGB/cglB Streptococcus pneumoniae TIGR4

97.633

100

0.976
  comGB/cglB Streptococcus mitis NCTC 12261

94.97

100

0.95
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.621

94.379

0.553
  comYB Streptococcus mutans UA159

58.621

94.379

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497

Multiple sequence alignment