Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FZN31_RS19940 Genome accession   NZ_CP043414
Coordinates   4199720..4201117 (+) Length   465 a.a.
NCBI ID   WP_105288066.1    Uniprot ID   -
Organism   Escherichia coli strain EC42405     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4194720..4206117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZN31_RS19915 (FZN31_20265) aroP 4195268..4196641 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  FZN31_RS19920 (FZN31_20270) ampE 4196684..4197538 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  FZN31_RS19925 (FZN31_20275) ampD 4197535..4198086 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FZN31_RS19930 (FZN31_20280) nadC 4198174..4199067 (+) 894 WP_032183211.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FZN31_RS19935 (FZN31_20285) pilA 4199270..4199710 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  FZN31_RS19940 (FZN31_20290) pilB 4199720..4201117 (+) 1398 WP_105288066.1 type II secretion system protein GspE Machinery gene
  FZN31_RS19945 (FZN31_20295) hofC 4201107..4202309 (+) 1203 WP_000157286.1 protein transport protein HofC -
  FZN31_RS19950 (FZN31_20300) guaC 4202344..4203387 (-) 1044 WP_161399928.1 GMP reductase -
  FZN31_RS24920 - 4203543..4203587 (-) 45 WP_120795372.1 protein YacM -
  FZN31_RS19955 (FZN31_20305) coaE 4203612..4204232 (+) 621 WP_001269512.1 dephospho-CoA kinase -
  FZN31_RS19960 (FZN31_20310) zapD 4204232..4204975 (+) 744 WP_001194743.1 cell division protein ZapD -
  FZN31_RS19965 (FZN31_20315) yacG 4204985..4205179 (+) 195 WP_000005043.1 DNA gyrase inhibitor YacG -
  FZN31_RS19970 (FZN31_20320) mutT 4205336..4205725 (-) 390 WP_105288067.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 51131.90 Da        Isoelectric Point: 6.3159

>NTDB_id=337040 FZN31_RS19940 WP_105288066.1 4199720..4201117(+) (pilB) [Escherichia coli strain EC42405]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQF
TVELAGNAVSFRIATLPCQGGEKVVLRLLQQVSQALDINTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSAL
QTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTL
HTNSTTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRT
ALFEVLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPYGE

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=337040 FZN31_RS19940 WP_105288066.1 4199720..4201117(+) (pilB) [Escherichia coli strain EC42405]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTTGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACCCAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGTGTATTGCATCCTTTACCGGATGTTTTACCGGATGTTTTACCGGATGCCG
GAGTCGCATTAACCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTC
ACTGTCGAACTGGCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGCCAGGGTGGTGAAAAGGTGGTATTAAG
GTTGTTACAGCAGGTGAGCCAGGCACTGGATATCAACACGCTGGGGATGCAGCCGTTACAACTGGCGGACTTTGCTCATG
CCTTGCAACAACCACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTG
CAAACGCTGAATACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGAC
GCAAATCCATCCGCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGA
TCGGAGAGATCCGCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTA
CACACCAATTCCACCACTGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCGGCGCTTAC
GCTGGTAATAGCCCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAG
ACAATGTATGGCCGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACG
GCCTTATTTGAAGTTCTGCCCATAACACCGTTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAAC
GCACGCCCGACAGGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAG
AGTTAATCCGCGTATTGGGAATGCCGTATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.742

83.441

0.415
  pilB Glaesserella parasuis strain SC1401

41.151

100

0.415
  pilB Vibrio campbellii strain DS40M4

48.469

84.301

0.409
  pilB Haemophilus influenzae 86-028NP

39.579

100

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.985

85.591

0.402
  pilB Vibrio cholerae strain A1552

46.465

85.161

0.396
  pilB Acinetobacter baylyi ADP1

45.293

84.516

0.383
  pilF Neisseria gonorrhoeae MS11

44.473

83.656

0.372
  pilB Acinetobacter baumannii D1279779

44.216

83.656

0.37
  pilB Haemophilus influenzae Rd KW20

43.478

84.086

0.366
  pilF Thermus thermophilus HB27

41.422

87.742

0.363