Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ENC22_RS25690 Genome accession   NZ_CP034836
Coordinates   5818074..5820209 (-) Length   711 a.a.
NCBI ID   WP_164887385.1    Uniprot ID   -
Organism   Hahella sp. KA22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5813074..5825209
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENC22_RS25675 (ENC22_25675) - 5814665..5816296 (-) 1632 WP_129498772.1 AAA family ATPase -
  ENC22_RS25680 (ENC22_25680) aroB 5816311..5817393 (-) 1083 WP_164887487.1 3-dehydroquinate synthase -
  ENC22_RS25685 (ENC22_25685) aroK 5817405..5817908 (-) 504 WP_370454968.1 shikimate kinase AroK -
  ENC22_RS25690 (ENC22_25690) pilQ 5818074..5820209 (-) 2136 WP_164887385.1 type IV pilus secretin PilQ Machinery gene
  ENC22_RS25695 (ENC22_25695) - 5820209..5820751 (-) 543 WP_241566884.1 pilus assembly protein PilP -
  ENC22_RS25700 (ENC22_25700) - 5820751..5821365 (-) 615 WP_127973457.1 type 4a pilus biogenesis protein PilO -
  ENC22_RS25705 (ENC22_25705) - 5821368..5821937 (-) 570 WP_127973458.1 PilN domain-containing protein -
  ENC22_RS25710 (ENC22_25710) pilM 5821937..5823001 (-) 1065 WP_127973459.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 711 a.a.        Molecular weight: 77358.95 Da        Isoelectric Point: 4.6478

>NTDB_id=333349 ENC22_RS25690 WP_164887385.1 5818074..5820209(-) (pilQ) [Hahella sp. KA22]
MKVYLRPLQYFVEKAMTARILRVWLSFLALAISGWAGATSLTGIEFAALPGDRTEIKLKFDSTPPEPKGYSIEQPARIAL
DLEGVVNALAERNHQLSKGNTQSVTILEAGGRTRVIINLIELVTYTSEVKGNTLVLRLGEGADSSVPSSATSATVAAQAE
ASAPAQDARRTAPVKPTISEVDFLRGEKGDGRILITLTNPKVSVDMSEEAGNIKLVFGGVTLPRDMQRRLDVTDFATPVR
VVDSFMEGGDAVVVIKPEGEYDYLAYQADNQFTLAVEPLTEEESEQRRKDKFPYTGEKLSLNFQDIEVRSVLQLIADFTG
LNLVASDTVGGRITLRLQNVPWDQALELILKTKGLDQRKVGNVLLVAPAEEIAARERLELEASKQVKELAPVRLEVIQIN
YAKAKDIVGLLKEDKDLISTRGFISSDERTNTISIRETADKVAQVRRLIQTWDIPVSQVLIEARIVRARTDAVLDLGVKW
GGGYEKRHGSSTDIRAGGSTQTIVELNNNETVELDPGAMVVDLGVTRANASSIALGFASNSYFLDLELSAFESDGKGEIV
AQPKIVTADRQTARIESGEEIPYQEASSSGATSVSFKKAVLALEVTPQITPDDKIIMDLLVNQDTRGATTAGIPSIDTNE
VQTQVLVGNGETIVLGGIFTAETSTTVTKTPFLGDIPYLGRLFTKKENTDERSELLIFITPKLITNGLIEN

Nucleotide


Download         Length: 2136 bp        

>NTDB_id=333349 ENC22_RS25690 WP_164887385.1 5818074..5820209(-) (pilQ) [Hahella sp. KA22]
ATGAAGGTTTACCTTAGGCCATTACAGTATTTTGTGGAGAAAGCAATGACAGCTCGCATCTTGAGAGTATGGTTGTCGTT
TTTAGCGCTAGCCATTAGTGGGTGGGCAGGCGCCACCAGTTTAACAGGCATTGAATTCGCGGCATTGCCAGGGGATCGTA
CAGAAATCAAATTAAAGTTTGACTCTACTCCTCCAGAACCCAAAGGGTACAGTATTGAGCAGCCTGCCAGGATTGCACTT
GATTTAGAAGGTGTCGTGAATGCCCTTGCTGAGCGTAACCATCAGCTGTCCAAGGGGAATACTCAAAGTGTTACTATTTT
AGAAGCTGGCGGGCGAACAAGAGTTATAATAAACCTTATTGAATTAGTAACTTATACTAGCGAAGTTAAAGGGAATACAT
TGGTTCTTCGTTTGGGTGAAGGGGCTGATTCCTCTGTACCTTCAAGTGCGACGTCAGCTACTGTTGCAGCCCAAGCTGAG
GCTTCAGCGCCAGCACAAGATGCTCGCAGGACGGCTCCTGTTAAGCCCACAATATCGGAGGTGGACTTTCTTCGAGGAGA
AAAAGGTGATGGCAGAATTCTGATTACTTTGACGAACCCTAAGGTGAGTGTCGATATGTCGGAAGAGGCTGGAAACATTA
AGCTTGTTTTCGGCGGGGTGACTTTACCTCGTGATATGCAGCGACGTCTTGATGTTACTGATTTTGCCACTCCTGTGCGT
GTAGTCGACTCCTTTATGGAAGGGGGAGATGCCGTCGTCGTCATAAAACCTGAAGGAGAATATGACTATTTGGCCTATCA
AGCTGATAATCAGTTCACCCTTGCTGTTGAACCGTTAACCGAAGAAGAGTCGGAACAGCGGCGTAAGGATAAATTCCCTT
ATACAGGAGAAAAGCTCTCTTTGAACTTCCAGGATATTGAGGTTCGTTCTGTATTGCAGTTAATCGCTGATTTTACAGGT
TTAAACTTGGTTGCCAGTGATACTGTGGGTGGGCGTATTACCTTGAGACTGCAGAATGTTCCATGGGACCAAGCCTTAGA
GTTGATACTTAAAACAAAAGGGCTAGATCAACGTAAGGTCGGAAACGTTTTGCTGGTTGCGCCGGCAGAAGAGATTGCTG
CTCGTGAGAGGTTGGAATTGGAGGCAAGTAAGCAAGTAAAAGAACTTGCACCGGTCAGACTTGAGGTCATTCAGATCAAC
TATGCCAAAGCCAAAGATATTGTAGGTTTGTTGAAGGAAGATAAGGACTTAATATCTACGCGAGGTTTCATCTCTTCTGA
TGAGAGGACAAATACGATTAGTATCAGGGAGACAGCTGACAAAGTCGCTCAGGTTCGTCGTTTAATTCAGACATGGGATA
TTCCTGTTAGCCAAGTACTAATTGAGGCGAGAATTGTTCGAGCAAGAACTGATGCGGTACTCGACTTGGGTGTGAAGTGG
GGAGGCGGTTATGAGAAGCGGCATGGTAGTAGCACAGATATTCGTGCAGGTGGCTCAACTCAGACAATAGTGGAATTGAA
CAATAATGAGACAGTTGAGTTAGACCCAGGAGCTATGGTTGTAGACTTGGGTGTTACTCGAGCGAATGCTAGTTCTATAG
CTTTAGGTTTTGCAAGTAACAGTTATTTCTTGGATTTGGAGTTGTCGGCTTTTGAGTCAGATGGGAAAGGAGAAATTGTA
GCTCAGCCTAAGATTGTTACTGCTGACCGGCAAACTGCCAGGATTGAATCGGGAGAGGAGATTCCCTATCAGGAGGCTTC
TTCTAGCGGCGCAACTTCAGTTTCGTTTAAAAAGGCAGTGTTAGCTTTAGAAGTGACTCCTCAGATCACCCCAGACGATA
AAATTATTATGGACTTATTGGTCAACCAGGATACCCGAGGCGCAACTACTGCCGGGATTCCAAGTATTGATACCAATGAA
GTGCAAACTCAGGTTTTGGTTGGTAATGGAGAAACTATTGTTTTAGGTGGAATATTTACAGCTGAAACTTCTACAACAGT
TACTAAAACTCCGTTCTTGGGTGATATTCCTTACTTGGGGCGTTTATTCACCAAGAAGGAAAATACAGATGAAAGGTCAG
AACTTCTGATCTTCATCACCCCCAAACTCATCACTAACGGCCTGATCGAAAACTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(52-137)

Bacterial type II/III secretion system short domain (Secretin_N)

(395-458)

Bacterial type II and III secretion system protein (Secretin)

(550-704)

Secretin and TonB N terminus short domain (STN)

(322-368)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.931

100

0.511
  comQ Acinetobacter baylyi ADP1

40.303

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.851

100

0.399
  pilQ Acinetobacter baumannii strain A118

38.851

100

0.399
  pilQ Legionella pneumophila strain ERS1305867

40.784

96.906

0.395
  pilQ Legionella pneumophila strain Lp02

40.435

97.046

0.392

Multiple sequence alignment