Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ENC22_RS25710 Genome accession   NZ_CP034836
Coordinates   5821937..5823001 (-) Length   354 a.a.
NCBI ID   WP_127973459.1    Uniprot ID   -
Organism   Hahella sp. KA22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5816937..5828001
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ENC22_RS25685 (ENC22_25685) aroK 5817405..5817908 (-) 504 WP_370454968.1 shikimate kinase AroK -
  ENC22_RS25690 (ENC22_25690) pilQ 5818074..5820209 (-) 2136 WP_164887385.1 type IV pilus secretin PilQ Machinery gene
  ENC22_RS25695 (ENC22_25695) - 5820209..5820751 (-) 543 WP_241566884.1 pilus assembly protein PilP -
  ENC22_RS25700 (ENC22_25700) - 5820751..5821365 (-) 615 WP_127973457.1 type 4a pilus biogenesis protein PilO -
  ENC22_RS25705 (ENC22_25705) - 5821368..5821937 (-) 570 WP_127973458.1 PilN domain-containing protein -
  ENC22_RS25710 (ENC22_25710) pilM 5821937..5823001 (-) 1065 WP_127973459.1 pilus assembly protein PilM Machinery gene
  ENC22_RS25715 (ENC22_25715) - 5823179..5825590 (+) 2412 WP_127973460.1 penicillin-binding protein 1A -
  ENC22_RS25720 (ENC22_25720) - 5825701..5826972 (-) 1272 WP_127973461.1 malic enzyme-like NAD(P)-binding protein -
  ENC22_RS25725 (ENC22_25725) rpmE 5827214..5827432 (-) 219 WP_127973462.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38264.43 Da        Isoelectric Point: 4.2460

>NTDB_id=333350 ENC22_RS25710 WP_127973459.1 5821937..5823001(-) (pilM) [Hahella sp. KA22]
MFGLFGKKKQPVLGLDISSTSVKLIELSQQGSRYRVDSYAVEPLPPNAVVEKNINDAEAVGEVISRVVSKARINTKQAAV
AVAGSAVITKVIQMSSSIREQDLESQIQIEADQYIPYPLDEVALDFEVVGPNEVNAEMMDVLLAACRRENVELREDALEI
GGLTTKVVDVEAYAMERAMGLIEAQLGTGDESQTVAVVDIGATMTTLSVLSDGKTIYTREQLFGGKQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYEQEVLHPFKEAVVQQVARSLQFFFSSTQFNEVDYIILAGGTASIPGIAELVQERVGTTTLVANP
FADMTVSSKVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=333350 ENC22_RS25710 WP_127973459.1 5821937..5823001(-) (pilM) [Hahella sp. KA22]
GTGTTTGGACTATTTGGAAAGAAAAAACAGCCGGTTTTAGGGCTTGATATCAGTTCTACATCGGTCAAGCTGATTGAACT
CAGTCAGCAGGGCAGTCGATATCGAGTGGATAGTTATGCCGTGGAGCCTTTGCCGCCGAACGCAGTCGTTGAAAAGAATA
TCAATGATGCCGAAGCAGTAGGTGAAGTGATCTCAAGAGTTGTTAGCAAAGCTCGGATCAACACCAAGCAGGCTGCGGTT
GCAGTCGCAGGTTCAGCGGTTATCACCAAAGTCATTCAGATGAGCTCTTCAATTCGAGAGCAGGATCTGGAGTCACAAAT
TCAAATCGAAGCAGACCAATATATCCCTTATCCGTTGGACGAGGTCGCTCTGGACTTTGAAGTAGTGGGTCCAAACGAAG
TCAACGCCGAAATGATGGATGTTTTGTTGGCTGCGTGCAGACGAGAAAATGTTGAGCTGCGTGAAGATGCTCTGGAAATT
GGCGGTCTCACCACGAAAGTGGTGGATGTAGAAGCGTACGCTATGGAACGAGCCATGGGACTTATAGAAGCGCAACTTGG
TACGGGGGATGAGTCTCAAACCGTGGCTGTTGTGGATATCGGCGCCACCATGACTACGTTGAGTGTGTTATCTGACGGCA
AGACTATCTATACGCGTGAACAGCTATTTGGCGGCAAGCAGCTAACGGAAGAAATACAGCGCCGTTATGGTCTGTCTCAA
GAGGAAGCCGGGCTGGCTAAAAAGCAGGGCGGTTTGCCTGACGACTACGAGCAGGAAGTCCTGCATCCTTTCAAAGAAGC
GGTTGTGCAGCAAGTGGCCAGATCGCTGCAGTTCTTTTTCTCGTCCACCCAGTTCAACGAAGTTGATTACATCATTTTAG
CGGGGGGGACAGCCTCAATTCCTGGTATCGCTGAATTGGTTCAGGAGCGGGTCGGAACCACAACGCTGGTGGCGAACCCA
TTTGCAGACATGACAGTGTCGTCAAAGGTTAATGCATCAGCATTAAGTAATGACGCTCCCTCCCTCATGATCGCGTGCGG
ATTGGCAATGAGGAGTTTTGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

44.633

100

0.446

Multiple sequence alignment