Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ELQ88_RS06430 Genome accession   NZ_CP034783
Coordinates   963005..964699 (-) Length   564 a.a.
NCBI ID   WP_138964222.1    Uniprot ID   A0A5E7UU09
Organism   Pseudomonas sp. MPC6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 958005..969699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELQ88_RS06400 (ELQ88_06400) - 958358..959290 (+) 933 WP_138964214.1 LysR family transcriptional regulator -
  ELQ88_RS06405 (ELQ88_06405) - 959367..960056 (+) 690 WP_138964216.1 energy-coupling factor ABC transporter permease -
  ELQ88_RS06410 (ELQ88_06410) yacG 960094..960294 (-) 201 WP_123367803.1 DNA gyrase inhibitor YacG -
  ELQ88_RS06415 (ELQ88_06415) coaE 960291..960914 (-) 624 WP_138964218.1 dephospho-CoA kinase -
  ELQ88_RS06420 (ELQ88_06420) pilD 960911..961783 (-) 873 WP_138964220.1 A24 family peptidase Machinery gene
  ELQ88_RS06425 (ELQ88_06425) pilC 961785..963002 (-) 1218 WP_128870806.1 type II secretion system F family protein Machinery gene
  ELQ88_RS06430 (ELQ88_06430) pilB 963005..964699 (-) 1695 WP_138964222.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ELQ88_RS06435 (ELQ88_06435) pilA 964925..965344 (+) 420 WP_138964224.1 pilin Machinery gene
  ELQ88_RS06440 (ELQ88_06440) - 965660..966013 (+) 354 WP_138964226.1 BON domain-containing protein -
  ELQ88_RS06445 (ELQ88_06445) - 966323..966619 (-) 297 WP_128870675.1 DUF2845 domain-containing protein -
  ELQ88_RS06450 (ELQ88_06450) - 966619..969570 (-) 2952 WP_138964228.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62499.44 Da        Isoelectric Point: 6.3306

>NTDB_id=332992 ELQ88_RS06430 WP_138964222.1 963005..964699(-) (pilB) [Pseudomonas sp. MPC6]
MNDIALSGLAKQLVLAELLSDKSAQQAYQQAQRNRISLVNYLVQNKLVNSRQVAEIASEHFGMALLDLHCLDKESQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLSTEAILVEDEKLTDAIEKFFDSHSTGLEDMADIDLDG
VDIESVDDNNRDAGQDSDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGMLREVAKPPIQLANRIASRLKV
MASLDISERRRPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLGFAQALRSFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKRPIEIPREALLKEGFPAER
IGSFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPALQRLIMAEGNSLEIDTQMRKDGFNDLRTSGLIKAMQGITSLEEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=332992 ELQ88_RS06430 WP_138964222.1 963005..964699(-) (pilB) [Pseudomonas sp. MPC6]
ATGAATGACATCGCCCTTAGCGGCCTGGCCAAGCAATTGGTCCTGGCCGAACTGCTCTCTGACAAAAGCGCGCAACAGGC
GTATCAGCAAGCCCAGCGCAACCGGATTTCCCTGGTCAACTACCTGGTGCAAAACAAACTGGTGAATAGCCGTCAGGTTG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCGCTGCTGGATCTCCACTGCCTGGATAAAGAGTCACAACCCAAAGGGCTG
GTCAGCGAGAAACTGGTCCGCCAGCACCACGCCCTGCCCCTCTGGCGACGTGGCAACAAGCTGTTCGTGGGGATTTCCGA
TCCAACCAATCACCAGGCCATCAACGATATCCAGTTCAGCACCGGCCTCAGCACCGAAGCCATTCTGGTCGAAGACGAAA
AACTCACGGACGCCATCGAGAAATTCTTCGACAGCCACAGTACCGGCCTGGAAGACATGGCCGACATTGACCTCGATGGC
GTGGACATCGAGTCGGTAGACGACAACAATCGGGACGCCGGCCAGGATAGCGATGACGCACCCGTGGTGCGCTTCGTCCA
CAAGATGTTGCTCGATGCGATCAAGAGCGGCTCTTCCGACCTGCATTTCGAGCCCTACGAAAAAACCTACCGCGTGCGGA
TGCGCACCGACGGCATGCTGCGCGAAGTCGCCAAACCGCCGATTCAACTGGCCAACCGCATCGCTTCGCGGCTGAAAGTC
ATGGCCAGCCTCGACATCTCCGAACGGCGTCGGCCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAAGTCCAT
CGACTTTCGGGTCAACACCCTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCGCGCAAA
TGGGCATCGATGCCCTCGGCTACGAACCCGACCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCACAGGGGATGATT
CTGGTCACCGGACCCACCGGCTCAGGCAAGACCGTGTCGCTCTACACCGGCCTGAATATCCTCAACACCGTGGACATCAA
CATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAGCAGGGCCTGG
GATTTGCCCAGGCGCTGCGCTCGTTTCTGCGACAGGATCCGGACGTCATCATGGTCGGCGAAATCCGCGACCTGGAAACG
GCGGAAATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTGTCGACCCTGCACACCAACAGCGCTGCGGAAACCCT
GACCCGCCTGCACAACATGGGCATCCAGGGCTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGCCTGGCGC
GCAAACTGTGCAGCCATTGCAAGAGACCGATCGAGATTCCCCGGGAGGCGCTGCTCAAGGAGGGTTTTCCTGCGGAACGC
ATCGGCTCGTTCACGATCTATGAACCGGTCGGTTGCGATCACTGCAACGGCGGTTATAAAGGGCGCGTAGGGATTTATGA
AGTGGTGAAGAACACACCCGCATTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATACCCAGATGCGCA
AGGACGGGTTCAACGACCTGCGCACGTCCGGGTTGATCAAGGCCATGCAGGGCATCACCAGCCTTGAAGAAATCAACCGG
GTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E7UU09

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.075

99.645

0.589
  pilB Acinetobacter baumannii D1279779

58.719

99.645

0.585
  pilB Legionella pneumophila strain ERS1305867

54.947

100

0.551
  pilB Vibrio cholerae strain A1552

51.327

100

0.514
  pilB Vibrio campbellii strain DS40M4

50.089

99.823

0.5
  pilF Neisseria gonorrhoeae MS11

49.733

99.468

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

50.545

97.518

0.493
  pilF Thermus thermophilus HB27

36.412

99.823

0.363

Multiple sequence alignment