Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EKO29_RS17920 Genome accession   NZ_CP034660
Coordinates   4186907..4188163 (+) Length   418 a.a.
NCBI ID   WP_126670150.1    Uniprot ID   A0A3S9Q9Y6
Organism   Colwellia sp. Arc7-635     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4181907..4193163
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKO29_RS17895 (EKO29_17895) ampD 4181943..4182545 (-) 603 WP_126670145.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EKO29_RS17900 (EKO29_17900) - 4182642..4183163 (+) 522 WP_126670146.1 TIGR02281 family clan AA aspartic protease -
  EKO29_RS17905 (EKO29_17905) nadC 4183164..4184051 (+) 888 WP_126670147.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EKO29_RS17910 (EKO29_17910) - 4184369..4184800 (+) 432 WP_126670148.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EKO29_RS17915 (EKO29_17915) pilB 4184989..4186695 (+) 1707 WP_126670149.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EKO29_RS17920 (EKO29_17920) pilC 4186907..4188163 (+) 1257 WP_126670150.1 type II secretion system F family protein Machinery gene
  EKO29_RS17925 (EKO29_17925) pilD 4188398..4189279 (+) 882 WP_126670151.1 A24 family peptidase Machinery gene
  EKO29_RS17930 (EKO29_17930) coaE 4189410..4190021 (+) 612 WP_126670152.1 dephospho-CoA kinase -
  EKO29_RS17935 (EKO29_17935) zapD 4190127..4190873 (+) 747 WP_126670153.1 cell division protein ZapD -
  EKO29_RS17940 (EKO29_17940) yacG 4191089..4191325 (+) 237 WP_126670154.1 DNA gyrase inhibitor YacG -
  EKO29_RS17945 (EKO29_17945) mutT 4191353..4191751 (-) 399 WP_126670155.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45340.72 Da        Isoelectric Point: 9.6432

>NTDB_id=332209 EKO29_RS17920 WP_126670150.1 4186907..4188163(+) (pilC) [Colwellia sp. Arc7-635]
MAISSTMKKKDKKSKVKVLDIYKWQGLNRKGKKVGGEINANSILELKAQLRKQGITPGRISKKAKPLFGIGGDKKILPVD
IAVLTRQIATMLGAGVPLVQTIEMIGSGHNNGNMQKLLATIGNKLQTGIPLSECLREHPAYFDDLYCDLVKSGEQSGALE
TIYDRVATYKEKAEALKAKIKKAMTYPISVLVIAFVVTAVLLIFVVPVFKDIFDSFGAELPGFTLLVLAISEFMQAYWYF
GLGGIFLAIFLFKKAHLNSQSFRDSVDKKILKIPIIGDVLKKAAVARYARTLSTTFAAGVPLPDALESAAGASGNAVFRE
AILDIRAEVTSGMQMNLAMRNCAIFPDMVIQMVAIGEESGAVDDMLAKVATVYEQEVDNAVDNLTTLLEPMIMAVLGVVI
GGLIIAMYLPIFQIGMVV

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=332209 EKO29_RS17920 WP_126670150.1 4186907..4188163(+) (pilC) [Colwellia sp. Arc7-635]
ATGGCTATTTCTTCTACAATGAAAAAAAAAGATAAAAAAAGTAAAGTTAAAGTACTTGATATCTATAAATGGCAAGGCTT
AAACCGAAAAGGAAAAAAAGTAGGCGGTGAGATTAATGCTAACAGCATACTAGAATTAAAAGCTCAGCTACGTAAACAAG
GTATTACCCCAGGAAGAATCAGTAAAAAAGCAAAACCTCTTTTTGGTATAGGCGGCGATAAAAAAATACTCCCTGTCGAT
ATCGCGGTGTTAACTCGCCAGATTGCCACTATGCTAGGGGCAGGTGTACCTTTAGTGCAAACGATTGAAATGATTGGTTC
GGGTCATAACAATGGTAATATGCAAAAGCTATTGGCAACTATCGGCAATAAATTACAAACAGGTATTCCACTTTCAGAAT
GCTTACGTGAGCACCCAGCCTATTTTGACGACCTCTATTGCGACCTAGTCAAATCTGGTGAGCAATCAGGTGCATTAGAA
ACCATCTATGACCGTGTTGCCACCTATAAAGAAAAAGCAGAAGCCCTCAAAGCGAAAATAAAAAAAGCGATGACTTACCC
TATATCGGTATTAGTTATCGCCTTTGTTGTTACCGCCGTATTGTTAATATTCGTCGTGCCGGTATTTAAAGATATATTTG
ATAGCTTTGGCGCTGAATTACCTGGCTTTACCTTGCTTGTACTGGCCATCTCTGAGTTTATGCAAGCGTATTGGTATTTT
GGTCTTGGTGGCATTTTTCTTGCTATTTTCCTATTTAAAAAAGCACACTTAAACAGTCAAAGTTTTCGAGATAGCGTTGA
TAAAAAAATATTGAAAATTCCTATTATTGGTGATGTTTTGAAAAAGGCCGCCGTTGCCCGCTATGCGCGAACATTATCAA
CCACTTTCGCAGCAGGCGTACCTTTACCTGATGCCTTAGAGTCAGCAGCTGGAGCTTCTGGTAATGCGGTGTTTCGTGAG
GCTATTTTAGATATTCGCGCTGAAGTCACCTCTGGCATGCAAATGAACTTGGCCATGCGTAATTGTGCGATTTTTCCAGA
TATGGTTATTCAAATGGTTGCTATTGGTGAAGAGTCGGGTGCCGTTGATGATATGCTGGCCAAAGTGGCGACCGTTTATG
AGCAAGAAGTCGATAATGCTGTTGATAATTTAACCACCTTACTTGAGCCCATGATCATGGCTGTACTTGGCGTTGTGATC
GGTGGCTTAATCATTGCGATGTATTTGCCGATATTCCAAATTGGTATGGTAGTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(289-410)

Type II secretion system (T2SS), protein F (T2SSF)

(85-207)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9Q9Y6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.302

95.215

0.574
  pilC Acinetobacter baylyi ADP1

53.96

96.651

0.522
  pilC Acinetobacter baumannii D1279779

53.218

96.651

0.514
  pilC Legionella pneumophila strain ERS1305867

53.5

95.694

0.512
  pilC Vibrio cholerae strain A1552

46.465

94.737

0.44
  pilG Neisseria meningitidis 44/76-A

43.928

92.584

0.407
  pilG Neisseria gonorrhoeae MS11

43.928

92.584

0.407
  pilC Vibrio campbellii strain DS40M4

42.821

94.976

0.407

Multiple sequence alignment