Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EKO29_RS17915 Genome accession   NZ_CP034660
Coordinates   4184989..4186695 (+) Length   568 a.a.
NCBI ID   WP_126670149.1    Uniprot ID   A0A3S9Q9V4
Organism   Colwellia sp. Arc7-635     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4179989..4191695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKO29_RS17890 (EKO29_17890) ampE 4180928..4181800 (-) 873 WP_126670144.1 beta-lactamase regulator AmpE -
  EKO29_RS17895 (EKO29_17895) ampD 4181943..4182545 (-) 603 WP_126670145.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EKO29_RS17900 (EKO29_17900) - 4182642..4183163 (+) 522 WP_126670146.1 TIGR02281 family clan AA aspartic protease -
  EKO29_RS17905 (EKO29_17905) nadC 4183164..4184051 (+) 888 WP_126670147.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EKO29_RS17910 (EKO29_17910) - 4184369..4184800 (+) 432 WP_126670148.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EKO29_RS17915 (EKO29_17915) pilB 4184989..4186695 (+) 1707 WP_126670149.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EKO29_RS17920 (EKO29_17920) pilC 4186907..4188163 (+) 1257 WP_126670150.1 type II secretion system F family protein Machinery gene
  EKO29_RS17925 (EKO29_17925) pilD 4188398..4189279 (+) 882 WP_126670151.1 A24 family peptidase Machinery gene
  EKO29_RS17930 (EKO29_17930) coaE 4189410..4190021 (+) 612 WP_126670152.1 dephospho-CoA kinase -
  EKO29_RS17935 (EKO29_17935) zapD 4190127..4190873 (+) 747 WP_126670153.1 cell division protein ZapD -
  EKO29_RS17940 (EKO29_17940) yacG 4191089..4191325 (+) 237 WP_126670154.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62325.51 Da        Isoelectric Point: 5.4215

>NTDB_id=332208 EKO29_RS17915 WP_126670149.1 4184989..4186695(+) (pilB) [Colwellia sp. Arc7-635]
MKGLHQQSSLLSALTKHHIVSSEMVSKISAGFIQQKKPFVRYLVEDNSINAEAIAATLSRSFGYPLIELEDFDTSLVPDG
VRNEKLIRKHHALPLFLRGKVLFVAMSDPTNHDALEEIQFNTGFSTELVLANEKALSSCIERVLEADSDALDITDMDTAE
LAGIDIQDEHQEDDDIGGSDKEDAPIVVYINKILLDAIRKGASDLHFEPYEKSFRIRFRIDGILTEVAKPPVSLASRMAA
RLKVMSKLDIAERRVPQDGRIKLALSKKKSIDFRVSTLPTMWGEKVVMRILDSSSAMLGIEMLGYAPEQKKIYMDALAQP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGINQVQINTRAGLTFPSALRSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVTIIIAQRLARRLCVKCKAPEHIPDNELLAQGF
LAEQLTNMTLYKPVGCEACTGGYKGRVGVYEVIKISETTANLIMTGANSLDIAAQCQREGYNNLRQSGLEKAALGVTSLE
EINRVTSS

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=332208 EKO29_RS17915 WP_126670149.1 4184989..4186695(+) (pilB) [Colwellia sp. Arc7-635]
ATGAAAGGTCTTCATCAACAATCTAGCCTACTTTCGGCCTTAACTAAACATCATATTGTATCTAGCGAAATGGTAAGTAA
AATTAGTGCTGGCTTTATTCAGCAAAAAAAACCTTTTGTACGATATTTAGTTGAAGACAACAGCATCAATGCCGAGGCTA
TTGCTGCCACTCTTTCTCGCAGCTTTGGCTATCCGTTAATAGAATTAGAAGATTTTGATACTAGCTTAGTTCCTGACGGC
GTACGGAACGAAAAATTGATCAGAAAACATCATGCACTGCCGTTATTCTTACGGGGTAAAGTGTTATTTGTTGCGATGTC
TGATCCGACTAATCATGATGCGTTAGAAGAAATACAATTCAATACTGGCTTTTCTACTGAGTTAGTACTCGCCAATGAAA
AAGCGCTGAGCAGTTGTATTGAAAGGGTGTTAGAAGCAGATAGTGATGCCTTAGACATAACTGATATGGATACTGCTGAG
TTAGCCGGTATCGATATTCAAGATGAACATCAAGAAGACGATGATATTGGCGGTAGCGATAAAGAAGATGCGCCGATTGT
TGTCTATATTAATAAAATATTACTTGACGCTATTCGCAAGGGTGCCTCTGATTTACATTTCGAGCCCTATGAAAAATCTT
TCCGTATTCGTTTTCGTATTGACGGTATTTTAACAGAGGTCGCTAAACCTCCAGTATCTTTAGCCTCACGAATGGCCGCA
CGCTTGAAAGTCATGTCAAAACTCGATATTGCTGAACGAAGAGTGCCACAAGATGGTCGGATTAAATTAGCGTTATCAAA
AAAGAAATCTATTGATTTTCGTGTCAGTACTCTACCGACTATGTGGGGTGAAAAAGTGGTTATGCGTATTCTTGATTCCT
CCAGCGCTATGCTAGGTATTGAGATGTTAGGCTACGCACCTGAACAAAAGAAAATCTATATGGATGCCTTAGCTCAACCA
CAAGGCATGATACTAGTCACCGGCCCTACAGGTTCAGGTAAAACGGTATCACTGTACACCGGTTTAAATATTTTAAATAC
TGAAGAGCGCAATATTTCAACAGCAGAAGATCCAGTTGAAATTAACCTTGAAGGCATTAATCAAGTACAAATTAATACTC
GTGCAGGCTTAACTTTTCCAAGCGCTCTGCGCTCATTCTTACGCCAAGATCCTGATATTGTGATGGTTGGTGAAATTCGC
GATTTAGAAACCGCAGAAATTGCTATTAAAGCAGCACAAACGGGTCACTTAGTCTTATCAACACTACATACTAACTCTGC
AGCAGAAACTTTGACCCGTTTATTGAATATGGGCGTTCCTTCATACAACGTTGCCAGCTCAGTCACCATTATTATTGCTC
AACGATTAGCACGTCGATTATGCGTTAAATGTAAAGCTCCAGAACATATTCCTGACAATGAGCTTCTCGCTCAAGGATTT
TTAGCTGAGCAACTGACTAATATGACATTGTATAAACCTGTGGGTTGTGAGGCATGTACCGGCGGTTATAAAGGGCGAGT
GGGTGTATATGAAGTAATAAAAATTAGTGAAACAACTGCTAATTTAATTATGACAGGAGCAAATTCTTTAGATATTGCCG
CTCAATGCCAGCGAGAAGGTTATAATAATCTCCGCCAATCTGGGCTAGAAAAAGCAGCATTAGGAGTTACTAGCTTAGAA
GAAATTAACCGAGTTACTAGTTCATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S9Q9V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.701

94.366

0.563
  pilB Acinetobacter baumannii D1279779

56.396

97.711

0.551
  pilB Legionella pneumophila strain ERS1305867

58.131

94.19

0.548
  pilB Vibrio cholerae strain A1552

51.499

99.824

0.514
  pilB Vibrio campbellii strain DS40M4

50.088

99.472

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

52.091

92.606

0.482
  pilF Neisseria gonorrhoeae MS11

48.474

98.063

0.475
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.112

94.366

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

41.4

88.028

0.364

Multiple sequence alignment