Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EHC64_RS03375 Genome accession   NZ_CP034289
Coordinates   651124..652347 (-) Length   407 a.a.
NCBI ID   WP_140103791.1    Uniprot ID   A0AAW8Q0C6
Organism   Vibrio parahaemolyticus strain 20140722001-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646124..657347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHC64_RS03335 (EHC64_03405) rpsP 646165..646413 (+) 249 WP_005379962.1 30S ribosomal protein S16 -
  EHC64_RS03340 (EHC64_03410) rimM 646442..646990 (+) 549 WP_005462552.1 ribosome maturation factor RimM -
  EHC64_RS03345 (EHC64_03415) trmD 647018..647761 (+) 744 WP_005462562.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EHC64_RS03350 (EHC64_03420) rplS 647803..648156 (+) 354 WP_005462554.1 50S ribosomal protein L19 -
  EHC64_RS03355 (EHC64_03425) yacG 648544..648738 (-) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  EHC64_RS03360 (EHC64_03430) zapD 648807..649547 (-) 741 WP_023624252.1 cell division protein ZapD -
  EHC64_RS03365 (EHC64_03435) coaE 649575..650189 (-) 615 WP_031847490.1 dephospho-CoA kinase -
  EHC64_RS03370 (EHC64_03440) pilD 650190..651059 (-) 870 WP_015297252.1 A24 family peptidase Machinery gene
  EHC64_RS03375 (EHC64_03445) pilC 651124..652347 (-) 1224 WP_140103791.1 type II secretion system F family protein Machinery gene
  EHC64_RS03380 (EHC64_03450) pilB 652372..654057 (-) 1686 WP_140103792.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EHC64_RS03385 (EHC64_03455) - 654054..654512 (-) 459 WP_029847971.1 pilin -
  EHC64_RS03390 (EHC64_03465) nadC 654778..655665 (-) 888 WP_140103793.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EHC64_RS03395 (EHC64_03470) ampD 655758..656309 (+) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45220.36 Da        Isoelectric Point: 10.3313

>NTDB_id=330449 EHC64_RS03375 WP_140103791.1 651124..652347(-) (pilC) [Vibrio parahaemolyticus strain 20140722001-1]
MKTTTPQLKNFRWKGVNSSGKKTSGQTLAMSEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGKDITVFTRQISTML
VTGVPLVQALKLVSDNHKKAEMKSILMSVTRAVEAGTPMSKAMRTASNHFDPLYTDLIATGEQSGNLDEVFERLATYREK
NEQLRAKVIKALIYPAMVILVALGVSFIMLTKVIPEFEKMFVGFGAELPWFTRQVLDLSAWTQNWSPFIALGSISVCISA
RVLSKRSDSFRLMLNRSVLKFPVLGAVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=330449 EHC64_RS03375 WP_140103791.1 651124..652347(-) (pilC) [Vibrio parahaemolyticus strain 20140722001-1]
ATGAAAACTACGACACCACAATTAAAGAACTTCCGTTGGAAAGGCGTCAACAGCTCAGGGAAAAAGACCTCTGGTCAAAC
TCTGGCAATGAGCGAAATCGAAGTACGAGAGCGCTTAGATGCCCAGCACATCAAAATCAAAAAGCTAAAGAAAAGCAGCA
TCTCTTTTTTGACCAAACTTAGCCATCGCGTAAAAGGGAAAGACATCACGGTTTTCACTCGTCAAATCTCAACGATGCTA
GTAACCGGTGTGCCGCTAGTACAAGCATTGAAATTGGTCTCTGATAACCACAAAAAAGCGGAAATGAAATCCATTTTGAT
GAGTGTAACTCGGGCTGTTGAAGCGGGTACACCGATGTCCAAAGCGATGCGTACCGCCAGCAACCATTTTGACCCGCTGT
ATACCGACCTGATTGCGACAGGAGAACAATCGGGCAACTTAGACGAAGTGTTCGAACGCTTAGCTACCTACAGGGAAAAA
AATGAGCAGCTTAGAGCCAAAGTGATCAAGGCTCTAATATATCCAGCCATGGTTATTCTAGTTGCACTGGGAGTATCGTT
TATCATGCTAACCAAGGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGCTTTGGCGCTGAATTGCCATGGTTTACAAGGC
AAGTCTTAGATCTTTCCGCTTGGACACAAAACTGGAGCCCATTTATCGCACTTGGCTCCATCAGTGTATGTATCTCCGCG
AGAGTACTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTACTTAAGTTTCCAGTCCTTGGGGCAGT
TTTATCGAAAGCCGCTATCGCAAAATTTAGTCGAACACTCGCTACAAGCTTTACGGCGGGCATTCCGATTCTAACCAGTT
TAAAAACCACCTCGAAAACGTCCGGAAATATGCATTATCAATTGGCTATCGAAGAGGTTTATCGCGATACCGCAGCAGGA
ATGCCGATGTATGTTGCAATGCGCAATTGCAACGTGTTCCCTGAGTTGGTGCTGCAAATGGTTATGATAGGCGAAGAATC
CGGCCGACTTGACGATATGCTCAATAAAGTTGCCACAATTTACGAGTTTGAAGTGGACAACACCGTCGATAACCTCAGTA
AAATCTTAGAGCCATTGATTATCGTTTTTTTAGGTATCGTTGTCGGTGGCTTGGTCACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

85.995

100

0.86
  pilC Vibrio cholerae strain A1552

74.321

99.509

0.74
  pilC Acinetobacter baylyi ADP1

42.714

97.789

0.418
  pilC Acinetobacter baumannii D1279779

41.25

98.28

0.405
  pilG Neisseria gonorrhoeae MS11

40.247

99.509

0.4
  pilC Legionella pneumophila strain ERS1305867

40.049

100

0.4
  pilG Neisseria meningitidis 44/76-A

40

99.509

0.398
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment