Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   FP509_RS01100 Genome accession   NZ_CP041999
Coordinates   186900..187871 (+) Length   323 a.a.
NCBI ID   WP_000250805.1    Uniprot ID   Q8E236
Organism   Streptococcus agalactiae strain GBS11     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 181900..192871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FP509_RS01090 (FP509_01100) rpoC 182598..186248 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  FP509_RS01095 (FP509_01105) - 186362..186727 (+) 366 WP_000285373.1 DUF1033 family protein -
  FP509_RS01100 (FP509_01115) comGA 186900..187871 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  FP509_RS01105 (FP509_01120) comGB 187765..188808 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  FP509_RS01110 (FP509_01125) - 188805..188873 (+) 69 Protein_162 competence protein ComGF -
  FP509_RS01115 (FP509_01130) - 188882..190336 (-) 1455 WP_001044791.1 recombinase family protein -
  FP509_RS10920 - 190465..190755 (-) 291 WP_224212534.1 TM2 domain-containing protein -
  FP509_RS10925 - 190762..190935 (-) 174 Protein_165 TM2 domain-containing protein -
  FP509_RS01125 (FP509_01140) - 190965..191264 (-) 300 WP_000964068.1 hypothetical protein -
  FP509_RS01130 (FP509_01145) - 191347..192102 (-) 756 WP_000168828.1 helix-turn-helix transcriptional regulator -
  FP509_RS01135 - 192486..192629 (+) 144 WP_001113676.1 hypothetical protein -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36801.32 Da        Isoelectric Point: 6.8469

>NTDB_id=329470 FP509_RS01100 WP_000250805.1 186900..187871(+) (comGA) [Streptococcus agalactiae strain GBS11]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGIRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=329470 FP509_RS01100 WP_000250805.1 186900..187871(+) (comGA) [Streptococcus agalactiae strain GBS11]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTATAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAATTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E236

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Streptococcus mutans UA159

68.81

96.285

0.663
  comGA Streptococcus mutans UA140

68.81

96.285

0.663
  comGA Streptococcus gordonii str. Challis substr. CH1

63.636

98.762

0.628
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

95.975

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505