Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EHN06_RS04430 Genome accession   NZ_CP034142
Coordinates   980146..982221 (+) Length   691 a.a.
NCBI ID   WP_206075723.1    Uniprot ID   -
Organism   Marinobacter sp. NP-4(2019)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 975146..987221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHN06_RS04410 (EHN06_04410) pilM 977289..978353 (+) 1065 WP_127330520.1 pilus assembly protein PilM Machinery gene
  EHN06_RS04415 (EHN06_04415) - 978353..978916 (+) 564 WP_127330522.1 PilN domain-containing protein -
  EHN06_RS04420 (EHN06_04420) - 978916..979527 (+) 612 WP_127330524.1 type 4a pilus biogenesis protein PilO -
  EHN06_RS04425 (EHN06_04425) - 979530..980078 (+) 549 WP_127330526.1 pilus assembly protein PilP -
  EHN06_RS04430 (EHN06_04430) pilQ 980146..982221 (+) 2076 WP_206075723.1 type IV pilus secretin PilQ Machinery gene
  EHN06_RS04435 (EHN06_04435) aroK 982347..982913 (+) 567 WP_127330530.1 shikimate kinase AroK -
  EHN06_RS04440 (EHN06_04440) aroB 982906..984006 (+) 1101 WP_127330532.1 3-dehydroquinate synthase -
  EHN06_RS04445 (EHN06_04445) - 984044..985570 (+) 1527 WP_127330534.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 73844.99 Da        Isoelectric Point: 4.3425

>NTDB_id=329320 EHN06_RS04430 WP_206075723.1 980146..982221(+) (pilQ) [Marinobacter sp. NP-4(2019)]
MFRKLNVYVGVIALGLLSGLANAVTLEDVSFSSLPGDQLEVTLKFDGQPPEPTGYTIERPARIAVDLKGTTSGLDSRSLP
LGSGNAQSMTVVETKDRTRLIFNLVELTPYDTVRKGNALVLTIGGEGGAAAVASSGGSVSQSAVSSSPTSARDGALAGVD
FRRGKDGEGQVVVDLGSASAPVDLSELGGKIRLTMDGMTVPENLRRRLDVTDFATPVTRVDTYTEGGNAVVEIRPEGNYD
YIAYQSGSQFTVSVERLSEQEAEERREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVSGSITLRLQNVPW
DQALDLILKTKGLDKRQIGNVLLVAPADEIAAREKLELETSKQIAELAPVRLDIIQVNYAKAADIVALIQADEELISSRG
FVSSDARTNTISVRETAEKLEEIRRLVTTWDVAVRQVSIEARIVRAQTNVAENLGIRWGGAAFDVNGDNVVSIGGSQGAV
EEARQAAAGNNNTITFPGALAVDLGVTGEGASSFAIGWGSDDFLVDLELSALETDGRAEVVSQPRVVTADRQTAKIKSGE
EIPYQEASSSGATSVSFKEAALSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPAINTNEVTTQVLVGNGETVVLGGIF
QSEVATQTTKTPFLGDIPYLGRLFKRTEHIDERSELLIFITPKIIKSDLIQ

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=329320 EHN06_RS04430 WP_206075723.1 980146..982221(+) (pilQ) [Marinobacter sp. NP-4(2019)]
ATGTTCAGAAAACTCAATGTATACGTCGGCGTGATTGCTCTTGGGTTGTTGTCCGGCCTGGCCAATGCGGTCACGCTGGA
AGACGTGTCGTTTTCATCGTTACCTGGCGACCAGCTTGAAGTAACGTTGAAATTCGATGGTCAGCCACCCGAGCCTACGG
GTTATACCATAGAACGGCCTGCCAGGATTGCGGTTGACCTGAAGGGGACGACCAGTGGGCTGGATAGCCGAAGCCTGCCA
CTCGGCTCCGGAAATGCCCAGAGCATGACGGTGGTGGAAACCAAGGACCGGACCCGCCTGATTTTTAACCTGGTGGAGTT
AACCCCCTATGACACGGTGCGTAAGGGTAATGCTCTGGTCCTGACGATCGGTGGGGAAGGTGGTGCCGCTGCCGTGGCCT
CCAGTGGCGGTTCTGTCTCACAGAGTGCAGTCTCGTCATCGCCGACGTCCGCTCGTGACGGAGCACTGGCCGGGGTGGAT
TTTCGTCGCGGAAAGGATGGAGAAGGGCAGGTGGTTGTCGACCTGGGAAGCGCAAGTGCACCAGTCGATCTCTCTGAACT
TGGCGGCAAGATTCGCCTGACAATGGACGGAATGACCGTTCCCGAGAATCTCCGTCGTCGTCTGGACGTCACTGATTTCG
CCACTCCGGTTACCCGGGTGGATACCTATACCGAGGGTGGTAATGCGGTTGTCGAAATCCGCCCGGAGGGTAACTACGAC
TACATCGCCTATCAATCCGGTTCCCAGTTTACAGTGAGTGTCGAACGGCTGAGCGAGCAGGAAGCTGAAGAGCGTCGTGA
AGAAAAGTTTCCGTATACGGGCGACAAGCTGTCGCTCAACTTCCAGGATATTGAGGTGCGCTCGGTTCTGCAGTTGATTG
CGGACTTTACCGGCCTGAACCTGGTTGCCAGTGACACTGTGAGCGGAAGCATCACGCTGCGTTTGCAAAATGTTCCCTGG
GATCAGGCCCTGGATCTGATCCTGAAGACCAAGGGTCTCGACAAGCGCCAGATTGGTAACGTCCTTCTGGTGGCGCCGGC
CGACGAAATTGCGGCCCGGGAAAAGCTGGAGCTGGAAACCAGTAAACAGATTGCCGAACTGGCTCCGGTGCGTCTGGATA
TCATTCAGGTCAATTATGCCAAGGCGGCGGATATCGTAGCTCTGATTCAGGCTGATGAAGAACTTATCTCGTCTCGTGGT
TTCGTCTCCTCCGATGCGAGGACCAATACCATCAGCGTTCGTGAAACCGCTGAAAAGCTGGAAGAGATTCGCCGGCTCGT
GACCACCTGGGATGTGGCGGTTCGCCAGGTATCCATTGAGGCCCGGATCGTGCGGGCGCAAACCAACGTTGCCGAGAATC
TCGGGATTCGCTGGGGTGGCGCTGCGTTTGATGTGAATGGTGATAATGTGGTGTCCATCGGCGGCTCCCAGGGCGCGGTT
GAAGAAGCGCGGCAGGCTGCGGCGGGCAACAATAATACGATTACCTTCCCCGGTGCGCTGGCTGTCGACCTTGGCGTTAC
TGGTGAGGGTGCCTCTTCGTTTGCCATCGGTTGGGGAAGTGATGACTTCCTGGTGGACCTTGAGCTGTCTGCACTGGAAA
CCGATGGTCGCGCGGAAGTGGTCTCCCAGCCTCGAGTGGTGACGGCCGATCGGCAGACCGCGAAAATCAAGTCTGGTGAA
GAGATCCCTTATCAGGAGGCTTCTTCCAGTGGTGCTACGTCGGTTTCGTTTAAGGAAGCAGCCCTGTCTCTGGAAGTGAC
GCCGCAGATTACGCCAGACGACAAGATCATTATGGATCTGGTCGTCAATCAGGATTCCCGTGGTGAGGTCACGGCGGGTA
TTCCGGCAATCAACACCAATGAGGTAACAACCCAGGTGTTGGTGGGTAACGGGGAGACAGTTGTGCTGGGTGGTATCTTC
CAGTCCGAAGTGGCAACCCAGACCACCAAGACGCCGTTCCTTGGGGATATCCCGTATCTTGGGCGCCTGTTCAAGCGGAC
GGAGCATATCGACGAGCGTAGCGAACTGTTGATCTTTATCACGCCCAAAATCATCAAGAGTGATCTGATACAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-686)

Secretin and TonB N terminus short domain (STN)

(300-346)

Bacterial type II/III secretion system short domain (Secretin_N)

(374-436)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.58

99.855

0.505
  comQ Acinetobacter baylyi ADP1

41.21

100

0.424
  pilQ Acinetobacter baumannii strain A118

39.973

100

0.424
  pilQ Acinetobacter baumannii D1279779

39.973

100

0.424
  pilQ Legionella pneumophila strain ERS1305867

40.444

97.685

0.395
  pilQ Legionella pneumophila strain Lp02

40.444

97.685

0.395

Multiple sequence alignment