Detailed information NTG0329319

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EHN06_RS04410
• Genome accession: NZ_CP034142
• Coordinates: 977289..978353 (+)
• Length: 354 a.a.
• NCBI ID: WP_127330520.1
• Uniprot ID: A0A3T0CQT7
• Organism: Marinobacter sp. NP-4(2019)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 972289..983353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EHN06_RS04395 (EHN06_04395)	rpmE	972803..973024 (+)	222	WP_127330514.1	50S ribosomal protein L31	-
EHN06_RS04400 (EHN06_04400)	-	973254..974519 (+)	1266	WP_127330516.1	malic enzyme-like NAD(P)-binding protein	-
EHN06_RS04405 (EHN06_04405)	-	974592..977093 (-)	2502	WP_127330518.1	penicillin-binding protein 1A	-
EHN06_RS04410 (EHN06_04410)	pilM	977289..978353 (+)	1065	WP_127330520.1	pilus assembly protein PilM	Machinery gene
EHN06_RS04415 (EHN06_04415)	-	978353..978916 (+)	564	WP_127330522.1	PilN domain-containing protein	-
EHN06_RS04420 (EHN06_04420)	-	978916..979527 (+)	612	WP_127330524.1	type 4a pilus biogenesis protein PilO	-
EHN06_RS04425 (EHN06_04425)	-	979530..980078 (+)	549	WP_127330526.1	pilus assembly protein PilP	-
EHN06_RS04430 (EHN06_04430)	pilQ	980146..982221 (+)	2076	WP_206075723.1	type IV pilus secretin PilQ	Machinery gene
EHN06_RS04435 (EHN06_04435)	aroK	982347..982913 (+)	567	WP_127330530.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38025.02 Da
• Isoelectric Point: 4.1243

>NTDB_id=329319 EHN06_RS04410 WP_127330520.1 977289..978353(+) (pilM) [Marinobacter sp. NP-4(2019)]
MFGLFGKKSSAVLGVDISSSSVKLLELSKNGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTGVKQVAV
AVSGSAVITKVIQMDGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSETNPDQVDVLLAACRKENVDIREDALEI
ASLTTKVVDVEAYALERAYTLIEPQLDSQGEELVVAIVDIGATMTTLSVLADGRTVYTREQIFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLTPFREAVVQQVARALQFFFGASQYNAVDYVILAGGTASIQGLTEMVEEKTGTPTLVANP
FSDMSVGSRVNASALASDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=329319 EHN06_RS04410 WP_127330520.1 977289..978353(+) (pilM) [Marinobacter sp. NP-4(2019)]
GTGTTCGGATTGTTTGGAAAAAAATCCAGTGCCGTCTTGGGTGTGGACATCAGCTCCAGTTCAGTCAAGCTGTTGGAACT
CTCAAAGAACGGAGACCGATACAAGGTCGAAAGTTACGCAGTGGAGCCTTTGCCGGCTAACGCTGTTGTTGAAAAAAATA
TCACAGATGTGGAAGCGGTCGGCGAAGTCCTCAAGCGCGTCGCCTCCAAATCCCGCACCGGCGTGAAGCAGGTCGCTGTT
GCTGTGTCGGGTTCTGCCGTCATAACCAAGGTTATTCAGATGGACGGCGGCCTTAATGAGTTTGAAATGGAAGACCAGAT
CGCGCTGGAGGCGGATCAGTATATTCCGTACCCTCTGGACGAAGTCGCTATCGATTTTGAGGTGCAGGGACCCTCCGAAA
CCAATCCTGATCAGGTCGATGTGCTCCTGGCGGCATGCCGTAAGGAAAACGTCGACATCCGTGAAGACGCGTTGGAGATT
GCGTCACTCACCACCAAGGTTGTTGATGTCGAGGCCTACGCACTTGAGCGGGCCTACACCTTGATTGAGCCTCAATTGGA
CTCCCAGGGGGAGGAATTGGTTGTGGCCATCGTCGACATCGGCGCCACTATGACCACCCTGAGTGTTCTGGCTGACGGCA
GGACGGTCTATACCCGTGAACAGATTTTCGGCGGCAAGCAGTTGACCGAGGAGATCCAGCGTCGCTATGGCTTGTCTGTG
GAAGAGGCCGGGCTCGCCAAGAAACAGGGGGGATTACCGGATGACTACGAGTCCGAAGTGCTGACCCCCTTCCGGGAGGC
CGTCGTGCAGCAAGTGGCCAGGGCACTCCAGTTCTTCTTTGGTGCAAGTCAGTACAATGCGGTGGATTACGTGATTCTGG
CCGGTGGTACTGCGTCGATACAGGGCCTGACAGAAATGGTCGAAGAGAAAACGGGAACGCCGACTCTGGTTGCCAACCCA
TTTTCCGATATGTCCGTAGGCTCCAGGGTCAACGCGTCAGCGCTTGCGAGTGATGCGCCCTCGTTGATGATTGCATGTGG
GCTGGCGATGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A3T0CQT7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.107	100	0.531
comM	Acinetobacter nosocomialis M2	53.107	100	0.531
comM	Acinetobacter baylyi ADP1	51.13	100	0.511
pilM	Legionella pneumophila strain ERS1305867	46.893	100	0.469