Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ECB94_RS07670 Genome accession   NZ_CP033577
Coordinates   1633319..1635028 (-) Length   569 a.a.
NCBI ID   WP_164491435.1    Uniprot ID   -
Organism   Vibrio mediterranei strain 117-T6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1628319..1640028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECB94_RS07650 (ECB94_07665) - 1628993..1629817 (-) 825 WP_006074827.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ECB94_RS07655 (ECB94_07670) - 1629941..1631392 (-) 1452 WP_124940360.1 SPOR domain-containing protein -
  ECB94_RS07660 (ECB94_07675) aroB 1631486..1632571 (-) 1086 WP_006074829.1 3-dehydroquinate synthase -
  ECB94_RS07665 (ECB94_07680) aroK 1632600..1633124 (-) 525 WP_038231065.1 shikimate kinase AroK -
  ECB94_RS07670 (ECB94_07685) pilQ 1633319..1635028 (-) 1710 WP_164491435.1 type IV pilus secretin PilQ Machinery gene
  ECB94_RS07675 (ECB94_07690) - 1635028..1635540 (-) 513 WP_006071131.1 pilus assembly protein PilP -
  ECB94_RS07680 (ECB94_07695) - 1635524..1636120 (-) 597 WP_124940362.1 type 4a pilus biogenesis protein PilO -
  ECB94_RS07685 (ECB94_07700) - 1636127..1636720 (-) 594 WP_006071134.1 PilN domain-containing protein -
  ECB94_RS07690 (ECB94_07705) pilM 1636721..1637704 (-) 984 WP_124940363.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62003.06 Da        Isoelectric Point: 4.5938

>NTDB_id=324109 ECB94_RS07670 WP_164491435.1 1633319..1635028(-) (pilQ) [Vibrio mediterranei strain 117-T6]
MEIQTVTRKMLSTWRGAVYLWALIISTDLIAMPQTNQLVDVSFELDKQKRAVVVVELSHVGSVVDISAESQGIKIVVKQT
QLASDKLAILDVTDFSTAVNQIELFDDSPDVRIFVETAMAVQYQYTMHGKQLKVTILPLSQQQSDEQKLFGSHSGKLTSI
NFQDIPVRSVLQLLADYNHFNLVASDSVQGNLTLRLDGVPWTEVLDIILRVKGLDKRVKGNIIMVAPKTELDLQEKQALE
KSRLAEQIGQLKSEIININYAKAADVAAMIGGDSDVSMLSERGSVAIDERTNALLVRELPENLRVIKEIIASLDIPVKQV
QIEARVVTIKEGNIEELGIRWGYTSINGDTTIGSTIEGNLASSGIGGGEVDIDDFLNVNLGALSDNAASIAFQVASLGSN
TLLDLELSALQSESKAEIISSPRLITTNKKSAYIEQGTEIPYLKSTSSGATAVEFKKAVLSLTVTPQITPDNRLILDLDV
TQDRVGEVLKTLEGETVAIDTQRIGTQVLVNDGETVVLGGIFQHAMTSKVDKVPVLGDIPGLGALFRRTYENIGKNELLI
FVTPKIITQ

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=324109 ECB94_RS07670 WP_164491435.1 1633319..1635028(-) (pilQ) [Vibrio mediterranei strain 117-T6]
ATGGAGATACAAACAGTGACGAGAAAAATGTTGTCCACTTGGAGGGGGGCTGTCTATTTGTGGGCGTTAATCATCAGTAC
TGATTTGATCGCCATGCCCCAAACTAATCAACTTGTTGATGTCTCATTTGAACTGGATAAACAGAAGCGTGCAGTAGTCG
TTGTTGAGCTTTCTCATGTTGGTAGTGTTGTAGACATTAGCGCCGAGTCGCAAGGAATAAAGATAGTGGTAAAGCAAACC
CAGCTAGCGTCAGACAAGCTGGCGATTTTGGATGTCACGGATTTTTCTACCGCAGTGAATCAGATAGAACTATTTGATGA
TAGTCCCGATGTTCGGATCTTTGTCGAGACCGCTATGGCAGTTCAATATCAATATACGATGCATGGTAAACAACTGAAAG
TGACAATTTTACCTCTGTCTCAGCAACAAAGTGATGAACAAAAATTGTTTGGTAGTCATAGTGGCAAGCTCACTTCAATC
AATTTCCAAGACATTCCTGTGCGCAGTGTATTACAGCTTCTGGCTGACTATAACCACTTTAATTTAGTGGCATCCGATAG
TGTCCAAGGCAACTTAACCTTGCGTTTAGATGGCGTGCCATGGACGGAAGTCCTCGACATTATCCTGCGAGTAAAAGGGT
TAGATAAACGGGTCAAGGGCAACATCATCATGGTGGCCCCTAAAACAGAGTTAGATTTACAAGAGAAGCAGGCGTTAGAA
AAGAGTCGCTTGGCCGAGCAAATCGGTCAACTAAAATCGGAAATCATTAATATTAACTATGCTAAAGCGGCGGATGTAGC
GGCCATGATTGGTGGAGATAGTGATGTCAGTATGTTATCTGAACGAGGTTCAGTTGCTATCGATGAGCGCACCAACGCCT
TATTGGTGCGAGAACTGCCTGAAAATCTTCGCGTGATTAAAGAGATTATTGCCTCACTCGATATACCCGTGAAACAGGTA
CAAATTGAAGCGCGAGTGGTCACTATCAAAGAGGGTAATATTGAAGAGTTGGGGATACGGTGGGGTTATACATCGATAAA
TGGTGACACCACGATCGGCAGTACGATAGAAGGCAACCTTGCTTCCTCTGGCATCGGTGGGGGAGAAGTGGACATTGATG
ACTTCCTTAATGTTAACTTGGGGGCGCTGTCTGACAATGCCGCCAGTATCGCATTTCAAGTAGCAAGCCTCGGCTCAAAT
ACGTTACTTGATCTAGAGTTGTCCGCATTACAAAGTGAATCGAAAGCCGAAATCATATCCAGTCCACGTCTTATCACTAC
CAATAAAAAGTCTGCTTATATAGAGCAGGGAACAGAAATTCCTTATTTGAAATCGACATCGAGTGGAGCGACAGCAGTTG
AGTTCAAAAAAGCGGTGCTTTCCTTGACGGTGACTCCGCAGATCACGCCCGACAATCGACTTATTCTCGACTTAGATGTG
ACTCAAGACAGGGTTGGTGAAGTGCTGAAAACCCTAGAGGGTGAAACTGTGGCGATTGATACTCAAAGGATAGGGACTCA
AGTTCTGGTTAACGATGGCGAAACTGTGGTGTTAGGCGGTATTTTTCAACATGCCATGACCAGCAAGGTAGATAAAGTGC
CAGTTCTTGGTGATATTCCGGGGTTAGGGGCATTATTTAGACGAACCTATGAAAACATCGGTAAGAATGAGTTACTGATC
TTCGTAACTCCTAAGATTATTACTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(254-319)

Bacterial type II and III secretion system protein (Secretin)

(409-567)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

64.134

99.473

0.638
  pilQ Vibrio cholerae strain A1552

64.134

99.473

0.638
  pilQ Vibrio campbellii strain DS40M4

65.688

95.782

0.629
  comQ Acinetobacter baylyi ADP1

39.261

99.824

0.392
  pilQ Legionella pneumophila strain Lp02

40.768

96.134

0.392
  pilQ Legionella pneumophila strain ERS1305867

40.768

96.134

0.392
  pilQ Acinetobacter baumannii D1279779

36.86

100

0.38
  pilQ Acinetobacter baumannii strain A118

36.86

100

0.38
  pilQ Pseudomonas aeruginosa PAK

37.411

99.121

0.371

Multiple sequence alignment