Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0812_RS03065 Genome accession   NZ_CP033137
Coordinates   604987..606672 (-) Length   561 a.a.
NCBI ID   WP_054824002.1    Uniprot ID   A0AAP9K919
Organism   Vibrio owensii strain 1700302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599987..611672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0812_RS03035 (D0812_03035) rplS 600418..600771 (+) 354 WP_005438036.1 50S ribosomal protein L19 -
  D0812_RS03040 (D0812_03040) yacG 601148..601348 (-) 201 WP_027726863.1 DNA gyrase inhibitor YacG -
  D0812_RS03045 (D0812_03045) zapD 601405..602145 (-) 741 WP_054824030.1 cell division protein ZapD -
  D0812_RS03050 (D0812_03050) coaE 602173..602787 (-) 615 WP_009707571.1 dephospho-CoA kinase -
  D0812_RS03055 (D0812_03055) pilD 602788..603657 (-) 870 WP_041058619.1 A24 family peptidase Machinery gene
  D0812_RS03060 (D0812_03060) pilC 603730..604953 (-) 1224 WP_009707569.1 type II secretion system F family protein Machinery gene
  D0812_RS03065 (D0812_03065) pilB 604987..606672 (-) 1686 WP_054824002.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0812_RS03070 (D0812_03070) pilA 606672..607076 (-) 405 WP_054824001.1 pilin Machinery gene
  D0812_RS03080 (D0812_03080) nadC 607341..608228 (-) 888 WP_054824000.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D0812_RS03085 (D0812_03085) ampD 608321..608872 (+) 552 WP_005438027.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D0812_RS03090 (D0812_03090) pdhR 609278..610045 (+) 768 WP_005438026.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62312.24 Da        Isoelectric Point: 5.5355

>NTDB_id=321740 D0812_RS03065 WP_054824002.1 604987..606672(-) (pilB) [Vibrio owensii strain 1700302]
MLTNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLNSGFFQSHELTEHLSSIFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSRLKEINQDELAS
LVEVGDDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESYNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITNTEH
IFKANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIAKGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=321740 D0812_RS03065 WP_054824002.1 604987..606672(-) (pilB) [Vibrio owensii strain 1700302]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCTGGAATACTGACTTTTTCCCAAGAAGAACTGTTACTTGAGCAAGC
TAAAGCTTCTGGTATATCAATGCCAGAGGCTTTGCTCAATTCTGGTTTTTTCCAATCTCATGAATTGACTGAGCACCTTA
GCTCCATCTTTGGGCTTTCGTGCACTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACGCTAGGTTTGCGAGAT
TTGATCACTCGGCATAATGCTCTGCCGCTTAATCGCACCTCTTCAACTTTGATTCTTGCAGTTGCCGATCCAACCAATCT
TCAAGCCGAAGACGACTTTCGTTTTGCTACCGGTTTACAAGTAGAGCTAGTTCTGGCTGATTTTCGAGAGCTGACCGCTG
CCATTCGGCGCTTATATGGCCGAGCACTTGGGCAGGAAAAGTCGAGGTTAAAAGAAATAAACCAAGATGAACTCGCAAGC
TTGGTTGAGGTCGGAGATGATGAGATTGAGAACATTGAAGATCTCAGTCAAGACGAATCCCCCGTCAGTCGTTATATCAA
CCAAATCTTACTCGATGCTGTACGCAAAGGCGCTTCCGATATTCACTTCGAACCCTACGAAAACATGTATCGAGTGCGCT
TACGTTGCGATGGCATCTTGATCGAAATTCAACAGCCTCCGAGCCACTTAAGCCGCCGTCTCTCTGCCCGAATAAAGATC
CTATCTAAGCTCGATATTGCTGAACGTCGCTTACCTCAGGATGGACGAATCAAACTTAAGTTAAACCAAGACACCGCGAT
TGATATGCGTGTCTCCACACTGCCAACTCTGTTTGGAGAAAAAATCGTACTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTTGGTTATAGCGACCAACAGAAGCAGCTTTATTTGGATGCTCTGCGTCGGCCACAAGGCATGATT
TTGATGACGGGTCCAACAGGCAGTGGCAAAACCGTTTCTCTTTACACAGGACTAAGCATTCTTAACAAACCAGAGATCAA
CATCTCGACTGCGGAAGACCCAGTGGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCGAAGATCGGTTTTG
GTTTTGCTGAAGCACTTCGTTCTTTCTTGCGTCAGGATCCTGATGTGGTCATGGTGGGTGAGATCCGAGACCTCGATACG
GCAGAAATCGCTATCAAAGCATCGCAAACTGGTCACCTGGTTCTTTCCACGTTACATACAAACTCGGCCGCTGAAACCGT
AATCCGTTTATCCAACATGGGTGTTGAAAGCTATAACCTTGCCTCCTCACTTAGCTTGATCATCGCCCAGCGGCTAGCGC
GTAAACTGTGCCCATACTGTAGACAACCGCAAGAACCAAATTCCCAACTGCAACATATCGGGATTACCAACACAGAGCAT
ATCTTTAAAGCCAATCCAGATGGTTGTAACGAGTGTACCCACGGTTATTCAGGCCGAACCGGCATCTATGAAGTGATGAA
GTTTGATGAATCCCTATCTGAGGCGCTCATAAAAGGAGCTTCAGTACACGAATTAGAAAAACTCGCTATCGCCAAAGGGA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCGTCACTAGCTTCAGCGAATTACAACGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.613

100

0.966
  pilB Vibrio parahaemolyticus RIMD 2210633

89.84

100

0.898
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.06

92.513

0.472
  pilB Acinetobacter baylyi ADP1

48.524

96.613

0.469
  pilB Legionella pneumophila strain ERS1305867

48.155

96.613

0.465
  pilF Neisseria gonorrhoeae MS11

42.73

100

0.43
  pilB/pilB1 Synechocystis sp. PCC 6803

36.7

100

0.389
  pilF Thermus thermophilus HB27

36.348

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.38

99.465

0.362

Multiple sequence alignment