Detailed information NTG0032141

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: PCC7424_RS14795
• Genome accession: NC_011729
• Coordinates: 3352612..3355359 (+)
• Length: 915 a.a.
• NCBI ID: WP_015955001.1
• Uniprot ID: B7KA48
• Organism: Gloeothece citriformis PCC 7424
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3347612..3360359

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCC7424_RS14775 (PCC7424_2998)	hmpF	3347859..3349466 (-)	1608	WP_015954997.1	pilus motility taxis protein HmpF	-
PCC7424_RS14780 (PCC7424_2999)	-	3349896..3351161 (+)	1266	WP_015954998.1	response regulator	-
PCC7424_RS14785 (PCC7424_3000)	pilH	3351494..3351865 (+)	372	WP_041237758.1	response regulator transcription factor	Machinery gene
PCC7424_RS14790 (PCC7424_3001)	-	3351999..3352529 (+)	531	WP_015955000.1	chemotaxis protein CheW	-
PCC7424_RS14795 (PCC7424_3002)	pilJ	3352612..3355359 (+)	2748	WP_015955001.1	methyl-accepting chemotaxis protein	Machinery gene
PCC7424_RS14800 (PCC7424_3003)	-	3355553..3356347 (+)	795	WP_015955002.1	MlaE family lipid ABC transporter permease subunit	-
PCC7424_RS14805 (PCC7424_3004)	-	3356467..3357339 (+)	873	WP_015955003.1	response regulator transcription factor	-
PCC7424_RS14810 (PCC7424_3005)	-	3357336..3357623 (+)	288	WP_015955004.1	circadian clock KaiB family protein	-
PCC7424_RS14815 (PCC7424_3006)	kaiC	3357699..3359219 (+)	1521	WP_015955005.1	circadian clock protein KaiC	-
PCC7424_RS14820 (PCC7424_3007)	-	3359653..3359838 (+)	186	WP_041238254.1	PCP reductase family protein	-

Sequence

Protein

• Length: 915 a.a.
• Molecular weight: 101151.75 Da
• Isoelectric Point: 4.1784

>NTDB_id=32141 PCC7424_RS14795 WP_015955001.1 3352612..3355359(+) (pilJ) [Gloeothece citriformis PCC 7424]
MTSGIDYVKQYGKANAAYCRGDLDEAVSIINELAQNYPDDPNVLLLRGHIFVSLQQYHLAQQQYQMVLEIAQQAELIDYA
NKGLQQISIVQPELDEEEMEFSIDQTEAIAVSPNFHEEDFSQMPSKSDFDEIDWDDAVFSEEEMDEPTMGSGQSDPFINP
FVQEEENSSQTQDPYYDPTFKLSSSPSSREESPEVSNFSWQNYHHTDEQDTDYLPFANEETEVYNPNGQISNIDEFSEMG
DSTLVVSSNLSGNSLKGPSEKLPKNVYSSQHFLRNDPEDYQDFQKESSPIQKPVNSDEFLEDLEVFNDDDLDGLSQFDIT
DVAQSLPDSGLFDRQPEELDMGIESSSLSAAAVENSSFSKLNWTEASGENTTTNLSTATGRFIKPTVEIEQGKLAGFKNL
PLSKKQWITAASAGVASIVSIFLVSSAMWIFSPKQPTQKVDSTATKTETASSNQKQSPPPSPQPKPDAKKPPSPSSQALP
VATISPFSKSILWMMLCTGGASFGATFFLSRLNTNQIKQSVNDLQTQFDTIYAGDFNVKASIYSEDELGQLSARFNQMAQ
AILTTTSEAQRRAAETEQQQEDLQRQVIRLLDDVEGAARGDLTVEAEVTADVLGAVADAFNLTIHSLREIVRQVKKAAEQ
VNKSSTDSESFARNQSSDALRMAEELAVTLNSVQMMTDSIQRVAENAREAEEVARTSSVTALRGGESVERTVAGILQIRE
TVSETARKVKRLAEASQEINKIVAVVSQIASRTNLLALNASIQAARAGEAGRGFAIVADEVRQLADRSAKSLKEIEQIVL
QIQSETGSVMTAMEEGIQQVIDVTERSEQAKRSLEDIIQVSNRIDTLVRSITADTVKQRENSREVAQVMQSVELTAQETS
QESQRVAGSLQKLVKISQDLLESVERFKVDQHELK

Nucleotide

• Length: 2748 bp

>NTDB_id=32141 PCC7424_RS14795 WP_015955001.1 3352612..3355359(+) (pilJ) [Gloeothece citriformis PCC 7424]
ATGACTTCAGGCATAGATTATGTTAAACAATATGGAAAAGCCAATGCAGCTTATTGTCGAGGGGACTTAGACGAAGCTGT
GTCAATTATTAATGAGTTGGCGCAAAATTATCCAGACGATCCCAATGTTCTGTTACTCCGAGGACATATTTTTGTGAGCT
TACAGCAATATCATCTAGCTCAACAACAATATCAAATGGTTCTGGAAATTGCCCAACAAGCTGAATTAATTGATTATGCT
AACAAAGGATTACAACAAATATCGATAGTTCAACCTGAATTAGACGAAGAGGAAATGGAGTTTTCTATCGATCAAACTGA
GGCAATTGCTGTTAGTCCCAATTTTCATGAAGAAGACTTTTCCCAAATGCCCAGTAAGTCTGATTTTGACGAAATAGATT
GGGATGATGCCGTATTTTCTGAGGAAGAAATGGATGAGCCGACTATGGGGAGCGGCCAATCCGACCCATTTATTAATCCT
TTTGTTCAAGAGGAGGAAAACTCATCACAGACTCAAGACCCCTATTATGATCCAACCTTTAAACTCTCTTCATCACCCTC
TAGTAGAGAAGAATCGCCGGAGGTAAGTAACTTTTCTTGGCAAAATTATCATCATACTGATGAGCAAGACACCGATTATT
TACCCTTTGCCAATGAAGAGACAGAAGTGTATAACCCTAATGGTCAAATCAGCAACATAGATGAGTTTTCAGAGATGGGA
GATTCCACCTTAGTTGTCTCGTCTAATTTGTCGGGAAATTCTTTAAAAGGACCTTCAGAAAAATTGCCGAAAAATGTCTA
CTCCTCCCAGCATTTTCTCCGGAATGATCCAGAAGACTATCAGGACTTTCAAAAAGAGTCTTCCCCAATTCAAAAACCAG
TTAACAGTGATGAATTCTTAGAAGATTTAGAAGTCTTTAATGATGATGATTTAGATGGACTCTCCCAATTTGATATTACG
GATGTAGCTCAATCTTTACCGGATTCGGGTCTGTTTGATCGTCAACCTGAAGAGTTAGATATGGGGATAGAATCCAGTTC
CCTGAGTGCTGCTGCTGTTGAAAACTCCTCCTTTAGTAAACTTAATTGGACTGAAGCTAGCGGAGAAAATACAACCACTA
ATTTAAGCACAGCAACAGGGCGATTTATTAAGCCAACGGTTGAAATTGAACAAGGAAAATTGGCCGGATTTAAAAATCTT
CCTCTGAGTAAAAAACAGTGGATTACGGCGGCTTCTGCGGGGGTAGCATCAATCGTGAGCATTTTTTTAGTCAGTAGTGC
TATGTGGATATTTAGCCCTAAACAGCCGACTCAAAAGGTTGATTCAACCGCCACCAAAACTGAAACCGCCTCTTCTAACC
AGAAACAATCCCCTCCCCCATCCCCACAACCTAAACCCGATGCTAAAAAACCTCCCTCCCCCTCTTCTCAAGCCTTACCT
GTAGCTACGATTTCTCCTTTTAGTAAATCTATATTGTGGATGATGCTTTGTACCGGTGGCGCAAGTTTTGGGGCGACTTT
TTTCTTGAGTCGACTGAATACCAATCAAATTAAGCAATCCGTTAATGATTTACAAACTCAATTTGATACGATCTATGCCG
GAGATTTTAATGTCAAAGCTAGCATTTATTCAGAAGATGAATTAGGGCAATTATCGGCCAGATTTAATCAAATGGCTCAG
GCTATTTTAACCACCACCAGCGAAGCTCAACGACGGGCAGCAGAAACTGAACAACAACAAGAAGATTTACAACGTCAGGT
GATTCGACTGCTCGATGATGTAGAAGGAGCGGCAAGAGGAGATTTAACGGTAGAAGCAGAAGTCACGGCGGATGTTCTTG
GGGCGGTAGCGGATGCCTTTAATCTCACTATCCATAGTCTGCGGGAAATCGTCCGACAAGTTAAAAAAGCCGCCGAACAG
GTCAATAAAAGCTCAACCGATAGTGAATCTTTCGCCCGCAATCAATCGAGCGATGCTCTGCGAATGGCGGAAGAATTAGC
TGTTACCCTCAATTCTGTTCAAATGATGACCGATTCGATTCAACGGGTAGCAGAAAATGCCAGAGAAGCAGAAGAAGTTG
CCCGCACCTCTTCGGTTACGGCACTTAGAGGGGGTGAATCCGTAGAACGAACCGTGGCCGGTATTCTCCAAATCCGTGAA
ACTGTATCGGAAACTGCGAGAAAGGTAAAACGATTAGCGGAAGCTTCCCAAGAAATTAATAAAATTGTGGCGGTTGTGTC
TCAAATTGCCTCTCGAACCAATTTATTAGCTCTTAATGCCTCTATTCAAGCGGCTAGGGCAGGAGAAGCTGGACGGGGGT
TTGCCATCGTCGCCGATGAAGTGCGACAATTAGCCGATCGCTCCGCTAAGTCTTTAAAAGAAATTGAACAAATCGTTTTA
CAAATCCAAAGTGAAACCGGTTCAGTTATGACAGCGATGGAAGAAGGGATTCAACAGGTGATCGATGTTACCGAGCGCTC
GGAACAAGCAAAACGCTCTCTAGAGGATATTATTCAAGTATCTAATCGGATTGATACCTTAGTGCGTTCAATTACGGCTG
ATACGGTAAAACAACGGGAAAATTCTCGGGAAGTGGCTCAGGTTATGCAGTCTGTTGAATTAACCGCCCAAGAAACCTCT
CAAGAATCCCAACGGGTAGCAGGTTCTCTGCAAAAATTGGTGAAGATTTCCCAAGATTTGCTCGAATCAGTGGAACGTTT
TAAAGTGGATCAACATGAGCTTAAATAA

Domains

Predicted by InterproScan.

• HAMP domain (HAMP) (514-562)

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (692-872)

3D structure

• AlphaFold DB: B7KA48

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	51.765	100	0.529