Detailed information NTG0320610

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D9842_RS12960
• Genome accession: NZ_CP033043
• Coordinates: 2594446..2595078 (-)
• Length: 210 a.a.
• NCBI ID: WP_121662904.1
• Uniprot ID: -
• Organism: Metabacillus litoralis strain Bac94
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2589446..2600078

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9842_RS12940	holA	2589789..2590811 (-)	1023	WP_121662900.1	DNA polymerase III subunit delta	-
D9842_RS12945	-	2591201..2591335 (+)	135	WP_121662901.1	YqzM family protein	-
D9842_RS12950	-	2591386..2593650 (-)	2265	WP_121662902.1	DNA internalization-related competence protein ComEC/Rec2	-
D9842_RS12955	-	2593800..2594360 (-)	561	WP_121662903.1	ComE operon protein 2	-
D9842_RS12960	comEA	2594446..2595078 (-)	633	WP_121662904.1	helix-hairpin-helix domain-containing protein	Machinery gene
D9842_RS12965	comER	2595168..2596001 (+)	834	WP_121662905.1	late competence protein ComER	-
D9842_RS12970	-	2596062..2596820 (-)	759	WP_121662906.1	class I SAM-dependent methyltransferase	-
D9842_RS12975	rsfS	2596817..2597173 (-)	357	WP_121662907.1	ribosome silencing factor	-
D9842_RS12980	yqeK	2597173..2597745 (-)	573	WP_121662908.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
D9842_RS12985	-	2597735..2598307 (-)	573	WP_121662909.1	nicotinate-nucleotide adenylyltransferase	-
D9842_RS12990	yhbY	2598326..2598619 (-)	294	WP_121662910.1	ribosome assembly RNA-binding protein YhbY	-
D9842_RS12995	aroE	2598613..2599455 (-)	843	WP_121662911.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 210 a.a.
• Molecular weight: 22920.76 Da
• Isoelectric Point: 4.2560

>NTDB_id=320610 D9842_RS12960 WP_121662904.1 2594446..2595078(-) (comEA) [Metabacillus litoralis strain Bac94]
MSIVQKYKKWIILLVSLLAVTILYYYFFNIQASSELVTTNVEGAYMETSVPDETTKGEQKTSENSEASTIVIDVKGAVQT
PGVYEISANARVHEIIEKAGGLSDQADEAAVNLASSLEDGMVVYIPQIGENKENPFQTTNEDKDSPKKININLATSEELQ
TLSGIGPAKAEAIISYREENGHFKSIEGLLEVSGIGEKSLEKLKEEVTVN

Nucleotide

• Length: 633 bp

>NTDB_id=320610 D9842_RS12960 WP_121662904.1 2594446..2595078(-) (comEA) [Metabacillus litoralis strain Bac94]
ATGAGTATCGTCCAAAAATACAAAAAATGGATTATCCTGTTAGTTAGTTTATTAGCAGTAACTATACTTTATTATTATTT
TTTTAATATCCAAGCCTCATCTGAACTTGTGACAACAAATGTTGAAGGTGCCTATATGGAAACAAGTGTGCCGGATGAAA
CAACTAAGGGTGAACAGAAAACAAGTGAGAATTCAGAAGCATCCACCATCGTTATTGATGTAAAGGGGGCTGTTCAAACA
CCTGGGGTTTATGAGATTTCAGCTAATGCAAGAGTACATGAAATTATTGAAAAAGCTGGAGGATTGAGTGATCAAGCTGA
TGAAGCGGCCGTAAATCTTGCTTCTTCATTAGAAGATGGGATGGTAGTTTATATTCCGCAAATCGGTGAAAATAAGGAAA
ATCCATTTCAGACTACTAACGAAGATAAGGACAGCCCTAAGAAAATCAATATTAACCTTGCAACAAGTGAGGAGCTTCAA
ACACTGTCAGGTATTGGACCTGCAAAGGCTGAGGCTATTATTTCATATAGAGAAGAAAACGGTCACTTTAAATCGATTGA
AGGGTTATTAGAAGTATCGGGAATTGGAGAAAAATCATTAGAGAAATTAAAAGAAGAAGTTACTGTTAATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (147-208)

• SLBB domain (SLBB) (71-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	47.196	100	0.481
comEA	Streptococcus thermophilus LMD-9	40.179	100	0.429
comEA/celA/cilE	Streptococcus mitis SK321	37.963	100	0.39
comEA	Latilactobacillus sakei subsp. sakei 23K	34.468	100	0.386
comEA	Lactococcus lactis subsp. cremoris KW2	37.209	100	0.381
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.574	100	0.376
comEA/celA/cilE	Streptococcus pneumoniae D39	36.574	100	0.376
comEA/celA/cilE	Streptococcus pneumoniae R6	36.574	100	0.376
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.648	100	0.367
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.981	100	0.367