Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C0073_RS02215 Genome accession   NZ_CP032839
Coordinates   446802..448508 (-) Length   568 a.a.
NCBI ID   WP_101530011.1    Uniprot ID   -
Organism   Aeromonas veronii strain FC951     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441802..453508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0073_RS02185 (C0073_002185) mutT 442311..442715 (+) 405 WP_101530006.1 8-oxo-dGTP diphosphatase MutT -
  C0073_RS02190 (C0073_002190) yacG 442792..442992 (-) 201 WP_213392112.1 DNA gyrase inhibitor YacG -
  C0073_RS02195 (C0073_002195) zapD 442999..443720 (-) 722 Protein_422 cell division protein ZapD -
  C0073_RS02200 (C0073_002200) coaE 443758..444372 (-) 615 WP_101530008.1 dephospho-CoA kinase -
  C0073_RS02205 (C0073_002205) pilD 444386..445258 (-) 873 WP_101530009.1 A24 family peptidase Machinery gene
  C0073_RS02210 (C0073_002210) pilC 445320..446561 (-) 1242 WP_101530010.1 type II secretion system F family protein Machinery gene
  C0073_RS02215 (C0073_002215) pilB 446802..448508 (-) 1707 WP_101530011.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  C0073_RS02220 (C0073_002220) - 448512..448883 (-) 372 WP_276209678.1 pilin -
  C0073_RS24425 - 448890..448937 (-) 48 Protein_428 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  C0073_RS02225 (C0073_002225) nadC 449223..450092 (-) 870 WP_101530013.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C0073_RS02230 (C0073_002230) - 450096..450566 (-) 471 WP_101530014.1 retropepsin-like aspartic protease -
  C0073_RS02235 (C0073_002235) ampD 450697..451272 (+) 576 WP_101530015.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C0073_RS02240 (C0073_002240) pdhR 451551..452315 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62589.92 Da        Isoelectric Point: 5.4257

>NTDB_id=319054 C0073_RS02215 WP_101530011.1 446802..448508(-) (pilB) [Aeromonas veronii strain FC951]
MTSSPNSGLAHGLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSAMEDFGFSFGLHTEALLVEENKLTTAIGKLLENDTDVLGMEDIDESEI
SDLEVSDDNSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=319054 C0073_RS02215 WP_101530011.1 446802..448508(-) (pilB) [Aeromonas veronii strain FC951]
ATGACCTCTAGCCCTAATAGCGGCCTGGCCCATGGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAACGCTA
TCTGAGCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCACTGG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCAATGGAAGATTTCGGCTTCAGCTTCGGGCTTCATACCGAGGCGCTGCTGGTGGAAGAGAACA
AGCTCACCACCGCCATCGGCAAGCTGCTGGAAAACGATACCGACGTGCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAGGTTTCCGATGATAACAGCCGGCTGGACGAGAGCGTCAATACTGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGACGCCATCAAGCGCGGAGCCTCCGACCTGCACTTCGAGCCATATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCATGAAATCGCTACCCCGCCGGTCAACCTCTCCAACCGCTTCTCCGCCCGC
CTCAAGGTGATGGCGCGACTCGATATTGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGACGACCGCCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCAACCCCAAGG
CAGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGTCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACTCTGACCCGAATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCAGAAGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACG
CCGCAGCAGTTGGCGGCGGGGCTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATTATGCAGGGGGCAAACTCCCTGCAAATCGCCG
CTATAGCGCAGAAAGAGGGGATGCGTACCCTGCGTACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTTACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.239

100

0.563
  pilB Acinetobacter baylyi ADP1

55.965

100

0.562
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

54.578

98.063

0.535
  pilB Vibrio campbellii strain DS40M4

51.957

98.944

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

39.076

99.12

0.387
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment