Detailed information NTG0316721

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: D6869_RS09690
• Genome accession: NZ_CP032526
• Coordinates: 1937947..1938603 (-)
• Length: 218 a.a.
• NCBI ID: WP_014735120.1
• Uniprot ID: -
• Organism: Lactococcus cremoris strain 4B0
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1932947..1943603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D6869_RS09665 (D6869_09785)	atpF	1933019..1933525 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
D6869_RS09670 (D6869_09790)	atpB	1933540..1934253 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
D6869_RS09675 (D6869_09795)	-	1934300..1934515 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
D6869_RS09680 (D6869_09800)	-	1934698..1935474 (-)	777	WP_011835694.1	alpha/beta hydrolase family protein	-
D6869_RS09685 (D6869_09805)	-	1935758..1937966 (-)	2209	Protein_1902	DNA internalization-related competence protein ComEC/Rec2	-
D6869_RS09690 (D6869_09810)	comEA	1937947..1938603 (-)	657	WP_014735120.1	helix-hairpin-helix domain-containing protein	Machinery gene
D6869_RS09695 (D6869_09815)	-	1938661..1940022 (-)	1362	WP_011835696.1	ABC transporter permease	-
D6869_RS09700 (D6869_09820)	-	1940019..1940951 (-)	933	WP_011835697.1	ABC transporter ATP-binding protein	-
D6869_RS09705 (D6869_09830)	-	1941571..1942131 (-)	561	WP_011835699.1	GNAT family N-acetyltransferase	-
D6869_RS09710 (D6869_09835)	-	1942305..1943483 (-)	1179	WP_014735121.1	SLC13 family permease	-

Sequence

Protein

• Length: 218 a.a.
• Molecular weight: 23674.86 Da
• Isoelectric Point: 5.1730

>NTDB_id=316721 D6869_RS09690 WP_014735120.1 1937947..1938603(-) (comEA) [Lactococcus cremoris strain 4B0]
MEKILDKLKEYWKIMLLVVCALIAGGILYIFTNSPKPAETLSIENLSSSSTKSSVSKFNSSSSEKNKNEIMVDLKGAVAK
PNVYQISSDERLVDLIRQAGGFTDQADPKSINLSAKLKDEEVIYVPKVGESSSSESTDSPTGSSVSNQVSTTSGPKVNIN
KADLTELQKLTGIGQKKAQDIIDFRMKNGDFKSIEDLGKVSGFGDKTLEKLKDEISID

Nucleotide

• Length: 657 bp

>NTDB_id=316721 D6869_RS09690 WP_014735120.1 1937947..1938603(-) (comEA) [Lactococcus cremoris strain 4B0]
ATGGAAAAAATTTTAGATAAATTAAAAGAATACTGGAAAATAATGCTTTTAGTTGTTTGTGCACTTATTGCTGGTGGAAT
TCTTTATATTTTCACCAATTCACCAAAGCCAGCTGAAACTCTGTCAATAGAAAATTTGAGCTCTAGTTCTACCAAAAGCT
CTGTCAGTAAATTTAATAGTAGCAGTAGTGAAAAAAATAAAAATGAAATTATGGTTGATTTAAAAGGAGCGGTTGCAAAA
CCTAATGTATACCAAATTTCGTCAGATGAGCGCCTTGTTGATTTAATTAGGCAAGCGGGAGGATTTACTGACCAAGCAGA
CCCAAAATCAATCAATCTGTCAGCGAAACTTAAAGATGAAGAAGTGATTTATGTACCAAAAGTTGGGGAAAGTTCAAGTT
CAGAAAGTACTGACAGTCCTACTGGTAGCTCTGTCAGTAATCAAGTTTCAACGACAAGTGGTCCAAAAGTAAATATCAAT
AAAGCAGACCTGACAGAATTACAAAAACTGACTGGAATTGGTCAAAAAAAAGCGCAAGATATTATTGATTTTCGCATGAA
AAATGGTGACTTTAAATCAATAGAGGATTTGGGTAAAGTATCTGGCTTTGGGGATAAAACATTAGAAAAACTGAAAGATG
AGATTTCTATTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (71-126)

• Helix-hairpin-helix motif (HHH_3) (154-215)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	98.165	100	0.982
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	41.41	100	0.431
comEA/celA/cilE	Streptococcus mitis SK321	41.778	100	0.431
comEA/celA/cilE	Streptococcus mitis NCTC 12261	40.529	100	0.422
comEA/celA/cilE	Streptococcus pneumoniae R6	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae Rx1	40.991	100	0.417
comEA/celA/cilE	Streptococcus pneumoniae D39	40.991	100	0.417
comEA	Latilactobacillus sakei subsp. sakei 23K	37.021	100	0.399
comEA	Staphylococcus aureus MW2	35.931	100	0.381
comEA	Staphylococcus aureus N315	36.866	99.541	0.367