Detailed information NTG0316471

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LLWM1_RS09300
• Genome accession: NZ_CP032500
• Coordinates: 1841730..1842377 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis subsp. lactis strain WM1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1836730..1847377

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLWM1_RS09270 (LLWM1_1783)	atpF	1836801..1837307 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
LLWM1_RS09275 (LLWM1_1784)	atpB	1837322..1838035 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
LLWM1_RS09280 (LLWM1_1785)	-	1838080..1838295 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
LLWM1_RS09285 (LLWM1_1786)	-	1838479..1839255 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
LLWM1_RS09290 (LLWM1_1787)	comEC	1839540..1841099 (-)	1560	WP_405023164.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LLWM1_RS13790 (LLWM1_03005)	comEC	1841117..1841749 (-)	633	WP_284198769.1	ComEC/Rec2 family competence protein	Machinery gene
LLWM1_RS09300 (LLWM1_1788)	comEA	1841730..1842377 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
LLWM1_RS09305 (LLWM1_1789)	-	1842437..1843798 (-)	1362	WP_058221762.1	ABC transporter permease	-
LLWM1_RS09310 (LLWM1_1790)	-	1843795..1844727 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
LLWM1_RS09315 (LLWM1_1791)	-	1844832..1845230 (-)	399	WP_004255236.1	hypothetical protein	-
LLWM1_RS09320 (LLWM1_1792)	-	1845334..1845897 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
LLWM1_RS09325 (LLWM1_1793)	-	1846070..1847248 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=316471 LLWM1_RS09300 WP_004255240.1 1841730..1842377(-) (comEA) [Lactococcus lactis subsp. lactis strain WM1]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=316471 LLWM1_RS09300 WP_004255240.1 1841730..1842377(-) (comEA) [Lactococcus lactis subsp. lactis strain WM1]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367