Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDG62_RS17695 Genome accession   NZ_CP032286
Coordinates   3100433..3102145 (+) Length   570 a.a.
NCBI ID   WP_087527054.1    Uniprot ID   -
Organism   Acinetobacter sp. WCHA55     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3095433..3107145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG62_RS17650 (CDG62_17655) nusA 3096017..3097501 (-) 1485 WP_087527052.1 transcription termination factor NusA -
  CDG62_RS17655 (CDG62_17660) rimP 3097541..3098065 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  CDG62_RS17685 (CDG62_17690) secG 3099019..3099348 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  CDG62_RS17690 (CDG62_17695) tpiA 3099362..3100153 (-) 792 WP_087527053.1 triose-phosphate isomerase -
  CDG62_RS17695 (CDG62_17700) pilB 3100433..3102145 (+) 1713 WP_087527054.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG62_RS17700 (CDG62_17705) pilC 3102176..3103402 (+) 1227 WP_087527055.1 type II secretion system F family protein Machinery gene
  CDG62_RS17705 (CDG62_17710) pilD 3103402..3104262 (+) 861 WP_087527056.1 prepilin peptidase Machinery gene
  CDG62_RS17710 (CDG62_17715) coaE 3104269..3104868 (+) 600 WP_087527057.1 dephospho-CoA kinase -
  CDG62_RS17715 (CDG62_17720) - 3104882..3105799 (-) 918 WP_162904047.1 DMT family transporter -
  CDG62_RS17720 (CDG62_17725) rlmB 3105832..3106581 (-) 750 WP_087527058.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  CDG62_RS17725 (CDG62_17730) - 3106724..3107047 (-) 324 WP_087527059.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63610.91 Da        Isoelectric Point: 4.8942

>NTDB_id=314757 CDG62_RS17695 WP_087527054.1 3100433..3102145(+) (pilB) [Acinetobacter sp. WCHA55]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDPQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNQCKATVDIPEQSLLEMGF
TSTDIQDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=314757 CDG62_RS17695 WP_087527054.1 3100433..3102145(+) (pilB) [Acinetobacter sp. WCHA55]
ATGTCAGTATCAAATGGTTTGCCTAAATTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAACAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTCCCTTATCTAATTGAAAACCACAAACTCTCTC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTCGGAGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAGCATCGGGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCGATGGATGCGATTCGCTTCAATTCTAAAATGAATATTGAAGCAATTATTG
TTGAACATCCTCAGCTTGAAAAACTGATCGAACAAAATTTTGCACAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAACAAGACCCTCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGCGATGAAGCTCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGACTTACACTTTGAGCCTTATGAAAAATCTT
ACCGTGTCCGTTATCGTGTAGATGGGGTTCTGCGTCATATTGCCAGCCCACCACTACAACTGGCGACTCGTCTTGCCTCT
CGCTTGAAAGTTATGTCACAAATGGACATCTCTGAAAAACGCGTTCCACAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGTAAAGCCATCGACTTTCGTGTCAACTCTCTTCCAACCTTGTTTGGTGAAAAATTGGTTCTGCGTATTCTCGACCCAT
CTAGTGCTATGTTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTGTTTATGCAAGCTTTGGACAAACCA
CAAGGCATGTTGTTAATTACTGGTCCGACAGGTTCAGGTAAAACGGTATCGCTATATACAGGTCTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAATGTGAACAATA
AAGTCGGCCTCACCTTCTCTGCTGCACTAAAGTCCTTCTTACGTCAGGATCCTGATATTGTGATGGTCGGTGAGATTCGT
GACCTTGAAACAGCTGAAATCGCGATTAAAGCCGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACACGCTTACGCAACATGGGGGTTCCATCTTTCAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGTCGCTTATGTAATCAATGTAAAGCCACTGTCGACATTCCCGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCCAAGATCCAGACTTCAAAGTTTATCAACCCGTGGGATGTGCTGAGTGTCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCTGAAGCATCTGCAAAGTTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.811
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

54.12

93.684

0.507
  pilF Neisseria gonorrhoeae MS11

49.644

98.596

0.489
  pilB Vibrio cholerae strain A1552

48.325

99.474

0.481
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.304

100

0.372
  pilF Thermus thermophilus HB27

36.778

100

0.368

Multiple sequence alignment