Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BEN74_RS09510 Genome accession   NZ_CP032279
Coordinates   1639768..1641477 (+) Length   569 a.a.
NCBI ID   WP_068912964.1    Uniprot ID   A0A3B7PY18
Organism   Acinetobacter sp. WCHAc010034     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1634768..1646477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEN74_RS09465 (BEN74_09465) nusA 1635378..1636862 (-) 1485 WP_068912957.1 transcription termination factor NusA -
  BEN74_RS09470 (BEN74_09470) rimP 1636898..1637422 (-) 525 WP_068912959.1 ribosome maturation factor RimP -
  BEN74_RS09500 (BEN74_09500) secG 1638358..1638687 (-) 330 WP_068912961.1 preprotein translocase subunit SecG -
  BEN74_RS09505 (BEN74_09505) tpiA 1638701..1639492 (-) 792 WP_068912962.1 triose-phosphate isomerase -
  BEN74_RS09510 (BEN74_09510) pilB 1639768..1641477 (+) 1710 WP_068912964.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BEN74_RS09515 (BEN74_09515) pilC 1641509..1642738 (+) 1230 WP_068912967.1 type II secretion system F family protein Machinery gene
  BEN74_RS09520 (BEN74_09520) pilD 1642784..1643620 (+) 837 WP_068912969.1 prepilin peptidase Machinery gene
  BEN74_RS09525 (BEN74_09525) coaE 1643630..1644238 (+) 609 WP_068912971.1 dephospho-CoA kinase -
  BEN74_RS09530 (BEN74_09530) - 1644235..1645155 (-) 921 WP_068913015.1 DMT family transporter -
  BEN74_RS09535 (BEN74_09535) rlmB 1645184..1645933 (-) 750 WP_068912973.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  BEN74_RS09540 (BEN74_09540) - 1646072..1646395 (-) 324 WP_068912975.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63224.31 Da        Isoelectric Point: 4.8140

>NTDB_id=314705 BEN74_RS09510 WP_068912964.1 1639768..1641477(+) (pilB) [Acinetobacter sp. WCHAc010034]
MTALQGSPRFTGFIRRLVEEGQVSAASMQQALLSAKKANQDIVPYLIHELKIPSLTIAETISAEFGEPIFDIAAYDANQI
VREDIDEKLITRYRVLPIFKHSNILFVATSNPTHIEAVDAIRFSSKMNTETIIVEHNKLEKLIEQNFAEDSTFDFDDDFD
LDIATDAPDQNADDGEEEKGEEAPIVKYINKLLVDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKALFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGVNQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKQAVEIPQQSLLEMGFT
EADTQSPEFQIYQPVGCSECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIQIADASDKAGFSNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=314705 BEN74_RS09510 WP_068912964.1 1639768..1641477(+) (pilB) [Acinetobacter sp. WCHAc010034]
ATGACAGCATTACAGGGTTCTCCAAGATTTACAGGATTTATTCGGCGCCTGGTCGAAGAAGGACAGGTATCTGCCGCCAG
CATGCAGCAGGCGCTGCTGAGTGCAAAAAAAGCCAATCAGGACATTGTCCCCTATCTGATCCACGAGTTGAAGATTCCGT
CGCTGACCATCGCCGAAACCATTTCAGCTGAATTCGGCGAACCGATTTTTGACATTGCAGCCTATGACGCCAATCAGATT
GTCCGCGAAGATATTGATGAAAAGCTGATTACCCGCTACCGCGTGCTGCCGATTTTCAAGCATTCCAATATCCTTTTTGT
CGCGACCAGCAATCCGACGCATATTGAAGCAGTCGATGCGATCCGCTTCAGCAGCAAAATGAACACTGAGACCATCATTG
TCGAGCACAATAAGCTGGAAAAGCTGATTGAGCAGAATTTTGCTGAAGACAGCACCTTTGATTTTGATGATGACTTCGAT
CTGGATATCGCCACGGATGCGCCCGATCAGAATGCCGATGACGGCGAAGAGGAAAAAGGCGAAGAGGCGCCGATTGTCAA
GTACATCAACAAGCTACTGGTCGATGCGATCCGCATGGGCGCCTCGGATCTGCATTTTGAGCCCTATGAAAAAATGTACC
GCGTGCGCTACCGCGTCGACGGCGTACTGCGCCAGATCGCCAACCCGCCGCTGCAGCTGGCCAACCGCCTGGCTTCGCGC
CTGAAAGTCATGTCGCAGATGGACATTTCTGAAAAACGCGTGCCGCAGGACGGCCGCATCAAGCTGAAGCTGTCGAAAAG
CAAGGCCATCGATTTCCGTGTCAACTCCCTGCCGACCCTCTTCGGCGAAAAGCTGGTGCTGCGTATCCTCGATCCGTCCA
GCGCCATGCTCGGCATTGACGCGCTCGGCTATGAGCCGGAACAGAAAGCGCTGTTCATGGAGGCGCTGGACAAGCCGCAA
GGCATGCTGCTGATTACCGGCCCAACCGGTTCCGGTAAAACCGTATCGCTGTATACCGGCCTGAACATTCTGAACCGTGA
AGACACCAACATTTCAACTGCCGAAGACCCGGTCGAAATCAACCTGGAAGGTGTGAATCAGGTCAACGTCAACAATAAAG
TCGGCCTGACTTTCGCCGCCGCCTTGAAGTCTTTCCTGCGCCAGGATCCGGACATTGTCATGGTCGGCGAAATCCGGGAT
CTGGAAACTGCCGAAATCGCCATTAAGGCCGCGCAGACCGGCCATATGGTGATGTCAACCCTGCATACCAACAGCGCGCC
GGAAACCTTAACCCGCCTGCGCAACATGGGCGTGCCCTCATTCAATATTGCAACATCCGTCAATCTGGTGATTGCGCAGC
GCCTGGCCCGCCGCCTCTGCCCGCAGTGCAAGCAAGCCGTAGAGATTCCGCAGCAAAGTCTGCTGGAAATGGGTTTTACT
GAAGCGGACACGCAAAGCCCAGAGTTTCAGATTTACCAGCCTGTGGGCTGCTCAGAGTGCCGCGAAGGCTATAAAGGCCG
TGTCGGCATTTATGAGGTAATGAAAGTGACGCCGGAGATTTCCCGTATTATCATGGAGGACGGCAACGCCATTCAGATTG
CCGATGCTTCGGATAAAGCCGGATTCAGCAATTTGCGCCGTTCGGGCTTAATTAAGGTCATGCAGGGGGTGACTTCGCTT
CAGGAAGTCAATCGCGTGACCAGTGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7PY18

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80

100

0.801
  pilB Acinetobacter baylyi ADP1

75.789

100

0.759
  pilB Legionella pneumophila strain ERS1305867

51.859

94.552

0.49
  pilF Neisseria gonorrhoeae MS11

49.554

98.594

0.489
  pilB Vibrio cholerae strain A1552

48.485

98.594

0.478
  pilB Vibrio campbellii strain DS40M4

48.654

97.891

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

48.649

97.54

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

34.142

100

0.371
  pilF Thermus thermophilus HB27

36.14

100

0.362

Multiple sequence alignment