Detailed information NTG0313844

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CDG60_RS16765
• Genome accession: NZ_CP032134
• Coordinates: 3314589..3315647 (-)
• Length: 352 a.a.
• NCBI ID: WP_087512124.1
• Uniprot ID: A0A3B7M5W2
• Organism: Acinetobacter chinensis strain WCHAc010005
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3309589..3320647

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDG60_RS16740 (CDG60_16740)	aroK	3309925..3310464 (-)	540	WP_087512129.1	shikimate kinase AroK	-
CDG60_RS16745 (CDG60_16745)	pilQ	3310500..3312620 (-)	2121	WP_171405530.1	type IV pilus secretin PilQ family protein	Machinery gene
CDG60_RS16750 (CDG60_16750)	pilP	3312681..3313205 (-)	525	WP_087512127.1	pilus assembly protein PilP	Machinery gene
CDG60_RS16755 (CDG60_16755)	pilO	3313205..3313924 (-)	720	WP_087512126.1	type 4a pilus biogenesis protein PilO	Machinery gene
CDG60_RS16760 (CDG60_16760)	pilN	3313921..3314589 (-)	669	WP_087512125.1	PilN domain-containing protein	Machinery gene
CDG60_RS16765 (CDG60_16765)	pilM	3314589..3315647 (-)	1059	WP_087512124.1	pilus assembly protein PilM	Machinery gene
CDG60_RS16770 (CDG60_16770)	ponA	3315813..3318389 (+)	2577	WP_087512123.1	penicillin-binding protein PBP1a	-
CDG60_RS16775 (CDG60_16775)	-	3318467..3319270 (-)	804	WP_087512122.1	putative RNA methyltransferase	-
CDG60_RS16780 (CDG60_16780)	mraY	3319351..3320469 (-)	1119	WP_087512121.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38854.41 Da
• Isoelectric Point: 4.4055

>NTDB_id=313844 CDG60_RS16765 WP_087512124.1 3314589..3315647(-) (pilM) [Acinetobacter chinensis strain WCHAc010005]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGRYWVESYALVPLPEGSVVEKNILNPEAVGDALERALNLANSQSTSAAM
AIPTSMVITKTIEMDADMTEDEREVQIRMDAEQYIPFPLDEVSLDFEVLPDRLANPSRVNVLLVATPIENIEARSEALEL
GGLTPKIADVESFAMENAFQVFSETLPMGANTVGILDIGHTMTTLSVLQNNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDAEVLEPYLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=313844 CDG60_RS16765 WP_087512124.1 3314589..3315647(-) (pilM) [Acinetobacter chinensis strain WCHAc010005]
GTGCTCAGGTTATATCGTAAGCCAAATAAGGGGTTAATGGGAGTAGATATCAGTTCTACTTCTGTTAAGTTACTGGAACT
ATCTGTAAAGAATGGTAGGTATTGGGTAGAGAGCTATGCACTTGTCCCATTACCGGAAGGCAGTGTTGTTGAAAAAAATA
TCCTTAACCCGGAAGCGGTTGGGGATGCGCTTGAACGTGCATTGAATCTGGCAAACTCGCAGTCAACATCTGCGGCAATG
GCTATTCCAACCTCAATGGTGATTACCAAAACGATTGAGATGGATGCTGATATGACAGAAGATGAACGTGAAGTTCAGAT
TCGGATGGACGCAGAACAATACATTCCTTTCCCACTTGATGAAGTCAGTCTGGATTTTGAAGTTTTACCGGATCGCCTGG
CAAATCCTTCACGTGTCAATGTTTTACTGGTTGCAACACCGATTGAAAATATTGAAGCACGTTCAGAAGCGCTGGAGTTG
GGTGGATTAACTCCAAAAATTGCAGATGTTGAAAGTTTTGCAATGGAGAACGCTTTTCAGGTGTTCTCTGAAACCTTGCC
TATGGGGGCAAATACAGTCGGTATCCTGGATATCGGTCATACCATGACCACTTTGTCTGTATTGCAGAATAACAAAATCA
TATATACCCGTGAGCAGGTGTTTGGGGGCAAACAGCTGACTCAGGAAATTCAGAACCGTTACGGTCTGTCTTTTGAGGAA
GCGGGGCGTGCTAAAAAGAGCCGGACTTTACCGGATGACTACGATGCTGAAGTGCTGGAGCCTTATCTTGAGGCTGTGGT
TCAGCAGGCAGCACGTTCATTACAGTTTTTCTTTTCATCTTCTCAGTTTAATGAAATTGACCATATTCTGCTTGCAGGGG
GTAATGCCAATATTCCGGGCCTTGCCAAACTGCTTCAACAGAAACTGGGCTACCGTGTCACGATTGCCAATCCATTCCTT
CAGATGGGCTTCTCACCGCAGATTGATATTAAAAAAATTGAGAATGATGCTTCCTCACTCATGGTGGCATGTGGTCTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A3B7M5W2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.511	100	0.855
comM	Acinetobacter nosocomialis M2	85.511	100	0.855
comM	Acinetobacter baylyi ADP1	79.545	100	0.795
pilM	Legionella pneumophila strain ERS1305867	39.831	100	0.401