Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CDG60_RS16755 Genome accession   NZ_CP032134
Coordinates   3313205..3313924 (-) Length   239 a.a.
NCBI ID   WP_087512126.1    Uniprot ID   A0A3B7M0B7
Organism   Acinetobacter chinensis strain WCHAc010005     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3308205..3318924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG60_RS16735 (CDG60_16735) aroB 3308820..3309899 (-) 1080 WP_087512130.1 3-dehydroquinate synthase -
  CDG60_RS16740 (CDG60_16740) aroK 3309925..3310464 (-) 540 WP_087512129.1 shikimate kinase AroK -
  CDG60_RS16745 (CDG60_16745) pilQ 3310500..3312620 (-) 2121 WP_171405530.1 type IV pilus secretin PilQ family protein Machinery gene
  CDG60_RS16750 (CDG60_16750) pilP 3312681..3313205 (-) 525 WP_087512127.1 pilus assembly protein PilP Machinery gene
  CDG60_RS16755 (CDG60_16755) pilO 3313205..3313924 (-) 720 WP_087512126.1 type 4a pilus biogenesis protein PilO Machinery gene
  CDG60_RS16760 (CDG60_16760) pilN 3313921..3314589 (-) 669 WP_087512125.1 PilN domain-containing protein Machinery gene
  CDG60_RS16765 (CDG60_16765) pilM 3314589..3315647 (-) 1059 WP_087512124.1 pilus assembly protein PilM Machinery gene
  CDG60_RS16770 (CDG60_16770) ponA 3315813..3318389 (+) 2577 WP_087512123.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 239 a.a.        Molecular weight: 26642.18 Da        Isoelectric Point: 5.1427

>NTDB_id=313842 CDG60_RS16755 WP_087512126.1 3313205..3313924(-) (pilO) [Acinetobacter chinensis strain WCHAc010005]
MSRDEFDELNQDAAVAPKKKMTLDKFVQQFNTLDPNNYGSWPLSVKITCWIFIALVICAIGYFVVIKPKLDAISSANAQE
QNLLNEFREKDSKLRNLQQYQLQLQQMEASFNQQLEQLPKETEIPGLVEDINVTGVNSGLKFKNIRLEGEVKQEFFIEQP
ISIEATGDYHAFGSFVSGIAALPRIVTLHDFTITASENKEKKSDVPQVSYTAKAKTYRYIGNTDNQAAGNAPAAAGGAK

Nucleotide


Download         Length: 720 bp        

>NTDB_id=313842 CDG60_RS16755 WP_087512126.1 3313205..3313924(-) (pilO) [Acinetobacter chinensis strain WCHAc010005]
ATGAGTCGCGATGAATTTGATGAGTTAAATCAGGATGCTGCTGTTGCTCCGAAAAAGAAAATGACGCTGGATAAATTTGT
TCAGCAGTTCAATACGCTTGATCCGAATAATTATGGCAGCTGGCCTTTATCCGTAAAAATCACCTGCTGGATTTTTATCG
CACTGGTGATCTGTGCAATTGGCTATTTTGTGGTGATCAAACCGAAACTTGATGCAATTTCCAGTGCCAATGCACAGGAA
CAGAATCTGCTGAATGAGTTCCGTGAAAAAGACTCCAAACTGCGTAATTTACAGCAGTATCAGTTGCAGTTGCAGCAAAT
GGAAGCGAGTTTTAACCAACAGCTTGAACAACTGCCGAAAGAAACAGAAATTCCTGGACTGGTTGAAGATATCAACGTGA
CAGGTGTGAATTCAGGACTGAAGTTTAAAAATATCCGTCTTGAAGGTGAAGTGAAGCAGGAATTCTTTATTGAACAGCCA
ATTTCCATTGAAGCAACGGGTGATTATCATGCCTTTGGTTCATTTGTCAGTGGTATTGCTGCACTTCCACGTATCGTTAC
TTTGCATGATTTTACAATTACAGCATCTGAGAACAAAGAGAAGAAATCTGATGTTCCTCAGGTCAGTTATACGGCAAAAG
CAAAAACTTACCGTTATATCGGTAATACAGATAATCAGGCGGCTGGTAATGCTCCAGCAGCTGCTGGAGGAGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7M0B7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

73.109

99.582

0.728
  comO Acinetobacter baylyi ADP1

73.077

97.908

0.715

Multiple sequence alignment