Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D1115_RS03020 Genome accession   NZ_CP032093
Coordinates   582491..583714 (-) Length   407 a.a.
NCBI ID   WP_128810217.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain CAIM 1831     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577491..588714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1115_RS02980 (D1115_02985) rpsP 577660..577908 (+) 249 WP_004410028.1 30S ribosomal protein S16 -
  D1115_RS02985 (D1115_02990) rimM 577936..578484 (+) 549 WP_128810210.1 ribosome maturation factor RimM -
  D1115_RS02990 (D1115_02995) trmD 578512..579255 (+) 744 WP_128810211.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  D1115_RS02995 (D1115_03000) rplS 579297..579650 (+) 354 WP_128810212.1 50S ribosomal protein L19 -
  D1115_RS03000 (D1115_03005) yacG 579917..580111 (-) 195 WP_128810213.1 DNA gyrase inhibitor YacG -
  D1115_RS03005 (D1115_03010) zapD 580175..580915 (-) 741 WP_128810214.1 cell division protein ZapD -
  D1115_RS03010 (D1115_03015) coaE 580942..581556 (-) 615 WP_128810215.1 dephospho-CoA kinase -
  D1115_RS03015 (D1115_03020) pilD 581557..582426 (-) 870 WP_128810216.1 prepilin peptidase Machinery gene
  D1115_RS03020 (D1115_03025) pilC 582491..583714 (-) 1224 WP_128810217.1 type II secretion system F family protein Machinery gene
  D1115_RS03025 (D1115_03030) pilB 583748..585433 (-) 1686 WP_128810218.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D1115_RS03030 (D1115_03035) - 585454..585906 (-) 453 WP_128810219.1 pilin -
  D1115_RS03035 (D1115_03040) nadC 586130..587017 (-) 888 WP_128810220.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D1115_RS03040 (D1115_03045) ampD 587110..587667 (+) 558 WP_128810221.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45031.25 Da        Isoelectric Point: 10.3923

>NTDB_id=313693 D1115_RS03020 WP_128810217.1 582491..583714(-) (pilC) [Vibrio alfacsensis strain CAIM 1831]
MKNKGLRLNNYIWKGINSSGKKASGHTLALSEFEVRERLAAQHIKVRKLKKRRISLLKKLTQRVKGKDITAFTRQISTML
ITGVPIVQALHLVAESHKKAEMKSILLSVTRAIEAGTPISKAMHNTSKQFDPFYTDLIATGEQSGNLPLVFERLATYREK
SEQLRSKVIKALIYPAMVVFAALAVSYLMLTQVIPEFETMFAGFGAELPLFTQIVLLVSAWTQKWGAYIGLVFISLAITS
RFVSQHSHSFRLLLARVSIALPILGPVLSKAAIAKFSRTLATSFSSGIPILLCLQTTAKTSGNLHYQLAINDIYHDTAAG
MPLYIAMRNSGVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLLIVFLGVVVGSLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=313693 D1115_RS03020 WP_128810217.1 582491..583714(-) (pilC) [Vibrio alfacsensis strain CAIM 1831]
ATGAAAAACAAAGGTTTACGACTCAACAACTATATCTGGAAAGGCATCAATAGCTCCGGAAAGAAAGCCTCTGGCCATAC
TCTTGCTCTCAGTGAATTCGAAGTTCGCGAACGCTTAGCTGCGCAACACATAAAAGTCAGAAAACTAAAAAAGCGTCGTA
TCTCTTTGCTTAAGAAATTAACCCAGCGTGTAAAAGGTAAAGATATCACAGCTTTTACCCGTCAGATTTCGACCATGCTA
ATAACAGGAGTTCCCATTGTTCAAGCATTACATCTTGTCGCTGAAAGCCATAAAAAAGCAGAAATGAAGTCGATTCTGCT
CAGTGTTACTCGAGCGATAGAAGCCGGAACCCCAATATCAAAAGCCATGCATAACACCAGCAAGCAATTTGATCCGTTTT
ATACCGATCTAATCGCCACAGGTGAACAATCGGGTAACTTACCTCTTGTTTTCGAACGTCTCGCCACTTATCGAGAAAAA
AGCGAACAGTTGCGCTCAAAAGTCATCAAAGCCTTAATTTATCCAGCGATGGTCGTATTCGCAGCGCTTGCTGTCTCTTA
TCTGATGTTGACTCAAGTGATTCCTGAATTCGAAACGATGTTTGCAGGCTTTGGCGCAGAGTTACCGCTATTTACTCAGA
TAGTACTATTAGTGTCTGCTTGGACGCAAAAATGGGGAGCGTATATTGGCTTAGTATTCATAAGCTTAGCTATCACCTCA
CGTTTCGTAAGCCAGCATTCTCACTCGTTTCGTTTACTGCTTGCACGTGTCAGCATAGCGCTTCCCATTTTAGGTCCCGT
GTTATCTAAAGCGGCCATTGCTAAATTCAGTCGAACACTCGCGACGAGCTTCAGTTCAGGGATCCCTATTCTACTCTGCT
TACAAACCACGGCTAAAACCTCAGGTAACCTCCATTATCAACTCGCAATTAATGACATTTATCACGATACAGCAGCAGGA
ATGCCCTTGTATATTGCCATGCGAAATAGCGGAGTCTTTCCTGAATTGGTGTTGCAAATGGTGATGATTGGTGAAGAATC
CGGACGCTTGGATGATATGCTAAACAAAGTCGCGACCATTTATGAGTTTGAAGTCGATAACACCGTCGATAATTTGAGTA
AAATTTTAGAGCCTCTACTCATTGTTTTTCTCGGGGTTGTCGTAGGAAGTCTTGTTACTGCAATGTACCTCCCTATCTTT
AACTTAATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

78.133

100

0.781
  pilC Vibrio cholerae strain A1552

68.408

98.771

0.676
  pilC Acinetobacter baumannii D1279779

39.853

100

0.4
  pilC Acinetobacter baylyi ADP1

40.704

97.789

0.398
  pilG Neisseria meningitidis 44/76-A

38.741

100

0.393
  pilG Neisseria gonorrhoeae MS11

38.741

100

0.393
  pilC Legionella pneumophila strain ERS1305867

39.348

98.034

0.386
  pilC Pseudomonas stutzeri DSM 10701

38.424

99.754

0.383

Multiple sequence alignment