Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   Ahae5227_RS15510 Genome accession   NZ_CP031988
Coordinates   3218650..3220365 (+) Length   571 a.a.
NCBI ID   WP_008942284.1    Uniprot ID   C0VHN5
Organism   Acinetobacter haemolyticus strain 5227     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3213650..3225365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Ahae5227_RS15475 (Ahae5227_15635) nusA 3214458..3215942 (-) 1485 WP_005088007.1 transcription termination factor NusA -
  Ahae5227_RS15480 (Ahae5227_15640) rimP 3215980..3216504 (-) 525 WP_005083847.1 ribosome maturation factor RimP -
  Ahae5227_RS15500 (Ahae5227_15660) secG 3217140..3217469 (-) 330 WP_004637686.1 preprotein translocase subunit SecG -
  Ahae5227_RS15505 (Ahae5227_15665) tpiA 3217482..3218273 (-) 792 WP_005088004.1 triose-phosphate isomerase -
  Ahae5227_RS15510 (Ahae5227_15670) pilB 3218650..3220365 (+) 1716 WP_008942284.1 type IV-A pilus assembly ATPase PilB Machinery gene
  Ahae5227_RS15515 (Ahae5227_15675) pilC 3220390..3221616 (+) 1227 WP_008942283.1 type II secretion system F family protein Machinery gene
  Ahae5227_RS17995 (Ahae5227_15680) pilD 3221616..3222473 (+) 858 WP_008942282.1 prepilin peptidase Machinery gene
  Ahae5227_RS15525 (Ahae5227_15685) coaE 3222475..3223080 (+) 606 WP_017395818.1 dephospho-CoA kinase -
  Ahae5227_RS15530 (Ahae5227_15690) - 3223077..3223988 (-) 912 WP_017394516.1 DMT family transporter -
  Ahae5227_RS15535 (Ahae5227_15700) rlmB 3224028..3224774 (-) 747 WP_004637678.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  Ahae5227_RS15540 (Ahae5227_15705) - 3224883..3225209 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63334.50 Da        Isoelectric Point: 5.1457

>NTDB_id=313183 Ahae5227_RS15510 WP_008942284.1 3218650..3220365(+) (pilB) [Acinetobacter haemolyticus strain 5227]
MSGLHTSPKFSGFIRRLVEDGHMSASTMQTAIDTAKKTQQDIVGYLIEHHRISQLTIAESISAEFGEPIFDLAVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAMRFNTKLNIEPIIVEHSKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVNQDSQKDESDETPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKSLFLEALDK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPIEIPKQSLLELG
FTEQDLADPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=313183 Ahae5227_RS15510 WP_008942284.1 3218650..3220365(+) (pilB) [Acinetobacter haemolyticus strain 5227]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTGGTCGAAGATGGTCATATGTCTGCATCGAC
AATGCAAACTGCAATCGATACAGCAAAAAAAACACAACAAGATATTGTTGGATATTTAATTGAACATCATCGTATCTCTC
AACTGACCATTGCAGAGTCAATCTCGGCAGAGTTTGGCGAACCTATTTTTGATTTAGCTGTTTTTGATACTGCTTTATTA
CCTCGTGAGCTTGCTGACAGCAAATTAATTCAAAAGCATCGGGTTGCCCCTCTCATTCAACGCGGGCAAATTCTTTATGT
CGCGACAAGCAATCCAACCAATATCGACGCGCTTGATGCGATGCGATTTAACACCAAACTCAATATTGAACCGATTATCG
TCGAACATAGTAAACTTGAAAAACTGATTGAACAGCAGTTTGGTGACTCTGGAAGTTTTGATTTTGGTGACAGTGAAGAC
TTTGACTTAGATATTGATGTCAACCAAGATTCGCAAAAAGATGAAAGTGATGAAACGCCTCAAGGGGATGAATCACCAAT
TGTAAAGTACATCAACAAGTTACTGATTGATGCCATTCGCATGGGTGCGTCTGATCTACATTTTGAACCCTATGAAAAAA
CCTATCGTGTTCGTTATCGCGTAGATGGTGTCTTAAGACAAATTGCGACGCCACCGTTGCAAATGGCAAATCGCTTAGCT
TCCCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGTCGTATTAAGCTCAAACTTTC
GAAAACCAAAGCGATTGACTTTCGTGTCAACTCACTGCCTACCCTATTTGGTGAAAAGCTAGTACTCCGTATTCTGGATC
CTTCCAGCGCCATGCTCGGAATTGAAGCCTTAGGCTATGAAGATGAACAAAAGTCGCTATTCCTAGAAGCATTGGATAAA
CCTCAAGGCATGCTGTTAATTACTGGCCCAACAGGTTCAGGTAAAACTGTGTCGTTATATACAGGTCTCAACATTCTAAA
TACTGAAGACACCAATATTTCTACTGCTGAAGACCCAGTGGAGATTAACTTAGAAGGAATCAATCAGGTTAACGTCAATA
CTAAGACAGGCCTTACCTTCTCTGCTGCGCTGAAAGCATTCTTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATT
CGTGACCTAGAAACAGCAGAAATTGCAATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCCACGCTACATACCAATAG
TGCACCTGAAACACTGACACGTTTACGCAATATGGGCGTTCCCTCTTTCAATATCGCAACTTCGGTCAATCTTGTGATTG
CACAACGCTTAGCCCGTCGTTTATGCTCACACTGTAAAGCACCCATAGAAATCCCCAAACAAAGCTTATTAGAACTCGGC
TTTACTGAACAGGATTTAGCTGACCCTGAATTGCAAATCTTTCAACCAGTCGGCTGCTCAGAATGTCGTGAAGGCTATAA
AGGCCGTGTCGGTATCTATGAGGTCATGAAGGTGACGCCTGAGATCTCTAAAATTATTATGGAGGATGGCAATGCTTTAC
AAATCGCAGCGGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGCTCAGGTTTAAAGAAGGTCATGCAAGGCATTACC
TCATTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C0VHN5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.961

100

0.82
  pilB Acinetobacter baylyi ADP1

77.233

100

0.772
  pilB Legionella pneumophila strain ERS1305867

53.846

93.345

0.503
  pilF Neisseria gonorrhoeae MS11

48.696

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.195

97.898

0.482
  pilB Vibrio cholerae strain A1552

49.275

96.673

0.476
  pilB Vibrio campbellii strain DS40M4

51.252

90.893

0.466
  pilF Thermus thermophilus HB27

37.478

98.599

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.364

Multiple sequence alignment