Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HYN24_RS02765 Genome accession   NZ_CP031842
Coordinates   554125..555840 (-) Length   571 a.a.
NCBI ID   WP_117607858.1    Uniprot ID   A0A3B7A9Q2
Organism   Dechloromonas sp. HYN0024     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549125..560840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HYN24_RS02740 (HYN24_02740) aspS 549272..551071 (-) 1800 WP_117607853.1 aspartate--tRNA ligase -
  HYN24_RS02745 (HYN24_02745) - 551089..551703 (-) 615 WP_117607854.1 DUF502 domain-containing protein -
  HYN24_RS02750 (HYN24_02750) - 551703..551957 (-) 255 WP_117607855.1 FmdB family zinc ribbon protein -
  HYN24_RS02755 (HYN24_02755) pilD 552029..552883 (-) 855 WP_117607856.1 A24 family peptidase Machinery gene
  HYN24_RS02760 (HYN24_02760) pilC 552883..554109 (-) 1227 WP_117607857.1 type II secretion system F family protein Machinery gene
  HYN24_RS02765 (HYN24_02765) pilB 554125..555840 (-) 1716 WP_117607858.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HYN24_RS02770 (HYN24_02770) - 556076..557347 (-) 1272 WP_117607859.1 HlyC/CorC family transporter -
  HYN24_RS02775 (HYN24_02775) - 557357..558196 (-) 840 WP_117607860.1 inner membrane protein YpjD -
  HYN24_RS02780 (HYN24_02780) ffh 558226..559584 (+) 1359 WP_117607861.1 signal recognition particle protein -
  HYN24_RS02785 (HYN24_02785) - 559585..560193 (-) 609 WP_117607862.1 lytic transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61982.54 Da        Isoelectric Point: 5.6155

>NTDB_id=311765 HYN24_RS02765 WP_117607858.1 554125..555840(-) (pilB) [Dechloromonas sp. HYN0024]
MAATPQNPPLGGLARALVQAGQLKEAEAEQLLAQAHSAKTSLIEQIIISQKAGALDIARFVADTFGYPLLDLAAFDEAHI
PTDAIDRKLIFTHKVIPLNKRGNRLSVAIADPTNLRALDEIRFQTGLAVDPIVVEQSKLAPLVAKYSESAADALKNFTSE
DINLDFLDEEAAAKVDDAAGQDIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKFYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMRLVLSKSRAIDFRVSTLPTLQGEKIVMRILDPTSATLGIEALGYEPEQKAVLMEAIA
RPYGMILVTGPTGSGKTVSLYTCLNILNKDGINISTAEDPAEINLPGVNQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEISIKAAQTGHLVLSTLHTNDAPQTLTRLMNMGVPMFNIASSVLLITAQRLARRLCTCKKPITVPEQALLDAG
YKQADLDGSWTLYGPGGCERCKGTGYKGRVGIYQVMPISEAMQRQILSGASALDLAKQAKDEGIKDLRESGLLKVKQGMT
SLDEVLSTTNA

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=311765 HYN24_RS02765 WP_117607858.1 554125..555840(-) (pilB) [Dechloromonas sp. HYN0024]
ATGGCAGCAACACCTCAAAATCCTCCACTCGGCGGCCTGGCACGAGCGCTCGTACAGGCCGGGCAACTCAAGGAGGCTGA
GGCAGAACAGCTACTGGCTCAAGCCCATAGCGCCAAGACTTCATTGATTGAGCAGATCATCATCAGCCAGAAGGCTGGCG
CGCTGGATATCGCCCGCTTCGTAGCCGACACCTTCGGTTATCCCTTGCTCGATCTCGCTGCCTTCGACGAGGCGCACATC
CCGACCGATGCCATCGACCGGAAACTTATTTTCACGCACAAGGTTATCCCGCTTAACAAGCGTGGCAACCGCCTGTCGGT
CGCTATCGCCGATCCGACCAATCTGCGCGCCCTCGACGAAATCCGCTTTCAGACCGGATTGGCGGTAGATCCGATCGTCG
TCGAGCAATCAAAACTGGCACCACTCGTCGCCAAATATTCCGAATCGGCAGCCGATGCCCTCAAGAATTTCACCAGCGAA
GACATCAATCTCGACTTTCTCGACGAGGAAGCTGCGGCCAAGGTCGATGATGCGGCAGGCCAGGACATTGACGATGCGCC
CGTCGTCAAATTTATCCAGAAAATGTTGCTCGACGCCATCAACGATGGCGCCTCTGACATCCATTTCGAGCCCTACGAAA
AGTTCTACCGTATCCGTTTCCGGGTAGACGGCATCCTGCGCGAAGTGGCGACGCCGCCGCTCGCCATCAAGGAAAAGATC
GCCTCGCGCATCAAGGTCATTTCCCGGCTGAACATTGCCGAAAAGCGGGTACCGCAAGATGGACGCATGCGTCTTGTGCT
ATCCAAGAGTCGCGCCATCGATTTCCGGGTCAGCACCCTACCGACCTTGCAGGGCGAAAAAATCGTCATGCGGATTCTTG
ACCCGACTTCGGCCACCTTGGGCATCGAGGCGCTCGGTTACGAGCCGGAACAAAAGGCCGTGCTGATGGAAGCCATCGCC
CGGCCCTACGGCATGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTCTATACCTGCCTGAATATCCT
GAACAAGGACGGCATCAACATTTCCACCGCTGAGGACCCGGCCGAAATCAATCTGCCCGGCGTCAACCAGGTGAATGTCG
ATGATCGCGCCGGACTGACCTTCCCGGTCGCGCTTAAAGCCTTCCTGCGCCAGGATCCCGACATCATCATGGTGGGCGAA
ATCCGCGATCTGGAAACCGCCGAGATTTCGATCAAGGCCGCCCAGACCGGTCACCTCGTGTTGTCCACGCTGCATACCAA
TGATGCGCCACAAACGCTTACCCGCCTGATGAACATGGGCGTCCCCATGTTCAACATCGCCTCCAGCGTTCTACTCATTA
CCGCGCAGCGTCTGGCCCGCCGGCTGTGCACCTGCAAGAAGCCGATCACCGTCCCCGAGCAGGCTCTCCTTGATGCCGGG
TACAAACAGGCCGATCTCGATGGCTCGTGGACCCTGTATGGCCCGGGCGGCTGTGAACGCTGCAAAGGAACAGGCTACAA
GGGCCGCGTCGGCATCTATCAGGTAATGCCGATTTCCGAGGCCATGCAACGGCAGATTTTGTCGGGCGCCTCTGCCCTTG
ATCTGGCGAAACAGGCCAAGGACGAAGGCATCAAGGATCTACGCGAATCTGGTTTATTGAAGGTCAAACAAGGCATGACG
TCGCTTGACGAAGTGTTGAGCACCACCAACGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3B7A9Q2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.07

100

0.552
  pilB Acinetobacter baumannii D1279779

53.31

100

0.536
  pilB Legionella pneumophila strain ERS1305867

52.174

100

0.525
  pilF Neisseria gonorrhoeae MS11

51.318

99.65

0.511
  pilB Vibrio cholerae strain A1552

48.301

97.898

0.473
  pilB Vibrio parahaemolyticus RIMD 2210633

48.128

98.249

0.473
  pilB Vibrio campbellii strain DS40M4

47.153

98.424

0.464
  pilF Thermus thermophilus HB27

39.341

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.652

98.774

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

41.036

87.916

0.361

Multiple sequence alignment