Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D0C16_RS11245 Genome accession   NZ_CP031728
Coordinates   2605662..2607386 (+) Length   574 a.a.
NCBI ID   WP_151032474.1    Uniprot ID   A0A5J6P7M5
Organism   Cellvibrio sp. KY-GH-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2600662..2612386
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0C16_RS11225 (D0C16_11415) - 2600763..2602346 (-) 1584 WP_151032470.1 NAD(P)/FAD-dependent oxidoreductase -
  D0C16_RS11230 (D0C16_11420) - 2602363..2602857 (-) 495 WP_151032471.1 GtrA family protein -
  D0C16_RS11235 (D0C16_11425) - 2602857..2604740 (-) 1884 WP_151032472.1 hypothetical protein -
  D0C16_RS11240 (D0C16_11430) - 2604894..2605385 (-) 492 WP_151032473.1 pilin -
  D0C16_RS11245 (D0C16_11435) pilB 2605662..2607386 (+) 1725 WP_151032474.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D0C16_RS11250 (D0C16_11440) pilC 2607391..2608674 (+) 1284 WP_151032475.1 type II secretion system F family protein Machinery gene
  D0C16_RS11255 (D0C16_11445) pilD 2608677..2609573 (+) 897 WP_151032476.1 A24 family peptidase Machinery gene
  D0C16_RS11260 (D0C16_11450) coaE 2609576..2610211 (+) 636 WP_151032477.1 dephospho-CoA kinase -
  D0C16_RS11265 (D0C16_11455) yacG 2610224..2610442 (+) 219 WP_370458210.1 DNA gyrase inhibitor YacG -
  D0C16_RS11270 (D0C16_11460) - 2610444..2611397 (-) 954 WP_151032478.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63067.27 Da        Isoelectric Point: 5.3479

>NTDB_id=310787 D0C16_RS11245 WP_151032474.1 2605662..2607386(+) (pilB) [Cellvibrio sp. KY-GH-1]
MNTPANSTALNGLARRLVADNLIDGEIAAKAMLQAKKDGIPFVDMLVSKEILDARTIARVAAEEFGSPVFDLTALNKESI
PLKLVDEKLIRKHHTLPIARRGNRLFLAVTDPTDIHALDEIKFNTGINTEAVLVEADKLKAAIEQYFNLQEGNLGDALGT
LDENLDELDVQTVDENNSDDTPEADEAPIVKYINKVLLDAIKGGSSDIHFEPYEKTYRIRFRTDGVLHEVSRPPVNLATR
MAARLKVMSQMDISERRLPQDGRIKLKVSKTRAIDFRVNTLPTLFGEKIVLRILDPSSAKLGIDALGYEDDQKRLYLDAL
AQSQGMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLEGINQVQMNTRVGLTFAEALRSFLRQDPDVIMVG
EIRDLETAEIAIKAAQTGHLVLSTLHTNSAPETLTRLLNMGVPAFNVATSVSLIIAQRLARRLCTACRKPATDIPEEILR
QEGFDEVSIPRSEFQLFHPVGCEKCNGGYKGRVGVYEVVRITPQIANLIMEGGNSLQIARAAKEAGFNNLRISALRKVAM
GLTSLEEANRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=310787 D0C16_RS11245 WP_151032474.1 2605662..2607386(+) (pilB) [Cellvibrio sp. KY-GH-1]
ATGAACACCCCAGCAAACTCCACTGCGCTTAACGGATTGGCGAGACGCTTGGTCGCAGACAACCTGATTGATGGCGAAAT
AGCCGCAAAGGCGATGCTTCAGGCAAAAAAAGACGGCATTCCTTTTGTTGATATGCTTGTCAGTAAAGAAATTTTGGATG
CGCGCACCATAGCTCGTGTCGCCGCAGAGGAATTTGGCTCACCGGTTTTTGACCTCACAGCGCTAAACAAAGAATCCATA
CCGCTAAAGCTGGTTGACGAAAAGCTCATTCGCAAGCATCACACCCTGCCAATCGCCAGAAGGGGCAACAGGCTATTTTT
AGCGGTTACAGACCCCACCGATATCCACGCACTGGATGAAATAAAGTTCAACACTGGAATCAACACGGAAGCTGTTCTGG
TAGAAGCAGACAAACTCAAAGCTGCGATCGAGCAATACTTTAACCTTCAAGAAGGTAACTTGGGTGATGCACTGGGAACG
CTCGATGAGAATCTTGACGAGCTGGATGTTCAGACAGTTGATGAGAACAACAGTGACGACACTCCCGAAGCCGATGAAGC
ACCTATCGTAAAGTACATTAACAAGGTATTGCTTGACGCGATAAAAGGTGGTTCATCAGACATTCACTTCGAGCCCTACG
AGAAAACTTACCGTATTCGCTTCCGTACAGATGGGGTACTCCATGAAGTCTCGCGACCACCGGTTAACCTTGCGACCAGG
ATGGCGGCACGCTTAAAAGTAATGTCGCAAATGGATATTTCCGAGCGACGTTTACCACAAGATGGACGCATAAAACTGAA
AGTTTCCAAAACTCGCGCGATAGATTTTCGGGTTAACACCCTGCCTACCCTCTTCGGTGAAAAAATTGTGCTGCGTATTC
TCGACCCCAGCTCTGCAAAGCTGGGAATCGATGCATTGGGTTATGAGGATGACCAAAAAAGACTCTACCTGGACGCATTG
GCACAATCGCAAGGAATGATTCTGGTTACCGGCCCCACCGGTAGTGGTAAAACTGTATCGCTTTATACGGGCCTAAATAT
TCTCAATACTTCTGAAGTCAACATTTCCACGGCAGAGGACCCGGTTGAGATAAACCTGGAAGGTATTAACCAGGTGCAAA
TGAACACAAGAGTTGGCTTAACCTTCGCGGAGGCACTCAGGTCTTTCTTGCGCCAAGACCCCGACGTTATCATGGTGGGG
GAGATTCGCGATCTTGAAACAGCTGAAATCGCGATTAAGGCGGCCCAAACTGGACACTTGGTATTGTCAACGCTACACAC
CAATTCGGCACCTGAAACGCTTACGCGCCTGCTAAATATGGGTGTACCCGCATTCAACGTGGCGACATCGGTGAGCTTGA
TTATCGCCCAACGCCTTGCCCGCCGTCTGTGCACCGCGTGTCGCAAACCTGCCACTGATATCCCGGAAGAGATACTTCGG
CAGGAAGGGTTTGACGAAGTCAGTATTCCGCGCTCAGAATTCCAGTTGTTTCATCCCGTTGGCTGCGAAAAATGCAATGG
GGGTTATAAAGGCCGGGTGGGTGTATATGAAGTGGTTCGCATCACCCCACAGATTGCCAACCTTATAATGGAAGGCGGCA
ACTCTTTACAAATTGCAAGAGCGGCCAAAGAAGCAGGCTTTAACAACCTGAGAATTTCCGCATTGCGTAAAGTCGCAATG
GGTTTAACCAGCCTTGAAGAGGCTAACCGAGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6P7M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.478

100

0.596
  pilB Acinetobacter baylyi ADP1

59.115

98.432

0.582
  pilB Legionella pneumophila strain ERS1305867

54.497

98.78

0.538
  pilB Vibrio cholerae strain A1552

52.593

94.077

0.495
  pilB Vibrio campbellii strain DS40M4

51.927

94.948

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.095

95.47

0.488
  pilF Neisseria gonorrhoeae MS11

47.636

99.477

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

39.512

100

0.423

Multiple sequence alignment