Detailed information NTG0310544

Bioinformatically predicted

Overview

• Name: pilF
• Type: Machinery gene
• Locus tag: D0T92_RS02730
• Genome accession: NZ_CP031700
• Coordinates: 609062..610741 (+)
• Length: 559 a.a.
• NCBI ID: WP_151049999.1
• Uniprot ID: A0A5J6PWT7
• Organism: Neisseria zalophi strain ATCC BAA-2455
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 604062..615741

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D0T92_RS02720 (D0T92_02755)	putA	604845..608438 (+)	3594	WP_151049997.1	bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA	-
D0T92_RS02730 (D0T92_02765)	pilF	609062..610741 (+)	1680	WP_151049999.1	type IV-A pilus assembly ATPase PilB	Machinery gene
D0T92_RS02735 (D0T92_02770)	pilG	610784..612031 (+)	1248	WP_318527727.1	type II secretion system F family protein	Machinery gene
D0T92_RS02740 (D0T92_02775)	pilD	612035..612898 (+)	864	WP_151050001.1	A24 family peptidase	Machinery gene
D0T92_RS02745 (D0T92_02780)	coaE	612900..613523 (+)	624	WP_151050003.1	dephospho-CoA kinase	-
D0T92_RS02750 (D0T92_02785)	zapD	613520..614287 (+)	768	WP_151050005.1	cell division protein ZapD	-
D0T92_RS02755 (D0T92_02790)	-	614291..614476 (+)	186	WP_151050007.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 559 a.a.
• Molecular weight: 62318.87 Da
• Isoelectric Point: 6.3000

>NTDB_id=310544 D0T92_RS02730 WP_151049999.1 609062..610741(+) (pilF) [Neisseria zalophi strain ATCC BAA-2455]
MSVGILRVLVQSKLIDSSQVEHYQNVLKSNKSILPLLFEENVITPKALGELLAKVFSYPLLDLSHYPRTNILSDVLTEEQ
MLQNRCIPIFQRGRKVYFAVSDPTQIQNFQKIGFTSGVSVDLVIVREDQLNTLLEWLGQRSTSILNEMSKEHEAAAAAQA
LFIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTESVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKAPMERPPEYALKKAGFTDEDLAKDWTM
YRAVGCDSCRGKGYKGRAGIYEVMPISEEMQRVIMENGTEVDIMNMAYKEGMVDLRRAGLLKVMQGITSLEEVLAHTND

Nucleotide

• Length: 1680 bp

>NTDB_id=310544 D0T92_RS02730 WP_151049999.1 609062..610741(+) (pilF) [Neisseria zalophi strain ATCC BAA-2455]
ATGAGTGTCGGTATATTAAGAGTATTGGTGCAAAGTAAGTTAATCGACAGTAGCCAGGTCGAACATTATCAAAATGTATT
AAAGTCTAATAAATCGATCCTTCCTTTGCTGTTTGAAGAAAATGTGATCACCCCAAAAGCCTTAGGCGAGTTGCTTGCCA
AGGTGTTCAGTTATCCTCTTTTGGATTTGAGCCATTATCCACGTACTAATATCTTGTCGGATGTGCTTACTGAAGAGCAG
ATGTTGCAAAACCGCTGTATTCCTATTTTTCAGCGGGGGCGGAAGGTTTATTTTGCTGTTTCCGATCCTACTCAAATTCA
AAATTTCCAAAAAATCGGGTTTACATCCGGTGTTTCCGTTGACTTGGTGATTGTACGGGAAGACCAGTTAAATACTTTAC
TCGAATGGTTGGGACAGCGCTCCACTTCCATCTTGAATGAAATGAGTAAAGAGCATGAAGCCGCAGCGGCCGCACAAGCT
TTATTTATTGATAATGAAGAGGCGGAAGACGGCCCGATTCCGCGTTTTATTCATAAAACCTTATCGGATGCATTGAATGC
GGGTGCTTCCGATATCCACTTCGAATTCTATGAGCAAATGGCACGCGTACGTTTCCGTGTCGACGGGCAATTGCGCGAAG
TGGTTCAACCACCCGTAGCTGTACGCGGACAGTTGGCATCGCGTATCAAAGTAATGGCACGCTTGGATATTTCCGAAAAA
CGGGTGCCGCAAGATGGACGTATTCAAATTGCATTCCATAAACACGGACGTCCGATTGATTTCCGTGTCAGCACATTGCC
GACTTTGTTCGGTGAAAAAGTAGTGATGCGTATTTTGAACTCTGATGCCGGCTCGTTGAATATCGACCAACTCGGATTCG
AGCCGTTCCAAAAAGAAATGCTGCTGGAGGCGATTCACCGCCCTTACGGCATGGTATTGGTAACTGGCCCGACGGGTTCG
GGTAAAACGGTATCGCTTTATACTTGTTTGAATATTCTGAATACAGAAAGCGTGAATATTTCCACAGCAGAAGATCCGGC
CGAGATTAATTTGCCCGGTATCAATCAGGTAAACGTAAATGATAAGCAGGGCTTGACCTTTGCTGCCGCTTTGAAATCCT
TTCTCCGTCAGGATCCCGATATCATCATGGTGGGTGAGATTCGTGACTTGGAAACTGCCGATATTGCTATTAAAGCAGCG
CAAACCGGCCATATGGTGTTTTCAACACTGCATACCAATAATGCCCCGGCAACTTTATCGCGTATGTTGAATATGGGGGT
GGCGCCATTTAATATTGCAAGCTCAGTCAGTTTGATTATGGCGCAGCGGCTGTTGCGTCGGCTGTGTCCGAATTGTAAAG
CCCCGATGGAACGTCCGCCTGAGTATGCTTTGAAAAAAGCTGGGTTTACCGATGAAGACTTAGCTAAAGATTGGACTATG
TACCGTGCCGTTGGTTGCGATAGCTGCCGCGGTAAGGGTTATAAAGGCCGTGCCGGTATTTATGAAGTCATGCCGATAAG
TGAAGAAATGCAGCGCGTTATTATGGAAAACGGCACAGAAGTAGATATTATGAATATGGCCTATAAAGAAGGTATGGTCG
ATTTGCGCCGTGCCGGTTTGCTGAAGGTGATGCAGGGGATTACTTCTTTGGAAGAAGTTCTGGCACATACTAACGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (37-140)

• Type II/IV secretion system protein (T2SSE) (179-447)

3D structure

• AlphaFold DB: A0A5J6PWT7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilF	Neisseria gonorrhoeae MS11	77.818	100	0.778
pilB	Acinetobacter baumannii D1279779	52.518	99.463	0.522
pilB	Acinetobacter baylyi ADP1	51.619	99.463	0.513
pilB	Legionella pneumophila strain ERS1305867	47.5	100	0.476
pilB	Vibrio parahaemolyticus RIMD 2210633	45.745	100	0.462
pilB	Vibrio cholerae strain A1552	45.907	100	0.462
pilB	Vibrio campbellii strain DS40M4	43.806	99.642	0.436
pilF	Thermus thermophilus HB27	36.918	99.821	0.369
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.74	93.739	0.363