Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CH582_RS03630 Genome accession   NZ_CP031689
Coordinates   718829..719278 (+) Length   149 a.a.
NCBI ID   WP_105894634.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P621-7028     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 713829..724278
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH582_RS03610 (CH582_03610) rsmE 714293..715030 (-) 738 WP_005686736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CH582_RS03615 (CH582_03615) lnt 715080..716609 (-) 1530 WP_116941799.1 apolipoprotein N-acyltransferase -
  CH582_RS03620 (CH582_03620) corC 716632..717531 (-) 900 WP_116941683.1 CNNM family magnesium/cobalt transport protein CorC -
  CH582_RS03625 (CH582_03625) ampD 718154..718714 (-) 561 WP_042599472.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH582_RS03630 (CH582_03630) pilA 718829..719278 (+) 450 WP_105894634.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH582_RS03635 (CH582_03635) pilB 719275..720669 (+) 1395 WP_105894635.1 GspE/PulE family protein Machinery gene
  CH582_RS03640 (CH582_03640) pilC 720666..721883 (+) 1218 WP_105894636.1 type II secretion system F family protein Machinery gene
  CH582_RS03645 (CH582_03645) pilD 721880..722572 (+) 693 WP_105894637.1 prepilin peptidase Machinery gene
  CH582_RS03650 (CH582_03650) rho 722626..723888 (-) 1263 WP_005648966.1 transcription termination factor Rho -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15398.73 Da        Isoelectric Point: 8.4623

>NTDB_id=310167 CH582_RS03630 WP_105894634.1 718829..719278(+) (pilA) [Haemophilus influenzae strain P621-7028]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTGKPSTCSGGSNGIA
ADITTAKGYVKSVTTSNGAITVKGDGTLANMEYILQATGNAAAGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=310167 CH582_RS03630 WP_105894634.1 718829..719278(+) (pilA) [Haemophilus influenzae strain P621-7028]
ATGAAACTAACAACACAGCAAACCTTGAAAAAAGGGTTTACATTAATAGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACTAAAAAAGCAGCGGTATCTGAATTACTGCAAGCGTCAGCAC
CTTATAAGGCTGATGTGGAATTATGCGTTTATAGCACAGGTAAACCTTCTACTTGCTCGGGAGGAAGCAATGGAATTGCA
GCAGATATAACGACAGCAAAAGGCTATGTAAAATCAGTGACAACAAGCAACGGTGCAATAACAGTAAAAGGGGATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAGCAGGTGTAACTTGGACAACAACTTGCAAAGGAA
CGGATGCCTCTTTATTTCCAGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)

Pilin (bacterial filament) (Pilin)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

94.631

100

0.946
  pilA Haemophilus influenzae Rd KW20

88.591

100

0.886
  pilA Glaesserella parasuis strain SC1401

56.376

100

0.564
  pilA2 Legionella pneumophila strain ERS1305867

39.865

99.329

0.396
  pilA2 Legionella pneumophila str. Paris

39.865

99.329

0.396
  pilA Vibrio campbellii strain DS40M4

39.007

94.631

0.369
  comP Acinetobacter baylyi ADP1

42.52

85.235

0.362

Multiple sequence alignment