Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DYQ48_RS05865 Genome accession   NZ_CP031607
Coordinates   1341151..1342896 (+) Length   581 a.a.
NCBI ID   WP_154844510.1    Uniprot ID   -
Organism   Xanthomonas hortorum strain VT106     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1336151..1347896
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DYQ48_RS05840 (DYQ48_05850) coaE 1337019..1337642 (-) 624 WP_154844506.1 dephospho-CoA kinase -
  DYQ48_RS05845 (DYQ48_05855) - 1337656..1338519 (-) 864 WP_002812278.1 A24 family peptidase -
  DYQ48_RS05850 (DYQ48_05860) pilC 1338526..1339782 (-) 1257 WP_154844507.1 type II secretion system F family protein Machinery gene
  DYQ48_RS05855 (DYQ48_05865) - 1340130..1340570 (+) 441 WP_154844508.1 pilin -
  DYQ48_RS05860 (DYQ48_05870) pilA 1340677..1341120 (+) 444 WP_154844509.1 pilin Machinery gene
  DYQ48_RS05865 (DYQ48_05875) pilB 1341151..1342896 (+) 1746 WP_154844510.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DYQ48_RS05870 (DYQ48_05880) pilR 1343240..1344694 (-) 1455 WP_174555994.1 sigma-54 dependent transcriptional regulator Regulator
  DYQ48_RS05875 (DYQ48_05885) - 1344780..1346393 (-) 1614 WP_006448368.1 HAMP domain-containing sensor histidine kinase -
  DYQ48_RS05880 (DYQ48_05890) sucC 1346626..1347795 (+) 1170 WP_006450728.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63030.16 Da        Isoelectric Point: 5.4464

>NTDB_id=307882 DYQ48_RS05865 WP_154844510.1 1341151..1342896(+) (pilB) [Xanthomonas hortorum strain VT106]
MNAGPANNNINLVGITGIARRLVQDGALDEASARSVMDQAANAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVF
DASQNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGS
SLGDDDDDMGDLDVSSGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVA
KAPVKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEAD
QQKLFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLR
QDPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKA
PLPEHALLAEGFTAEQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQS
ALVKAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=307882 DYQ48_RS05865 WP_154844510.1 1341151..1342896(+) (pilB) [Xanthomonas hortorum strain VT106]
ATGAATGCTGGGCCTGCCAATAACAATATTAATCTCGTAGGTATAACCGGCATTGCCCGGCGATTGGTCCAAGACGGTGC
CCTGGACGAAGCTAGTGCTCGCTCCGTCATGGATCAAGCAGCCAACGCAAAAGTTCCTTTGCCGCAATGGTTCGCCGAAA
AAAAGTTGGTGTCGGCAGCGCAGCTCGCCGCTGCCAATGCGGTTGAGTTTGGCATGCCCCTGATGGACGTTTCTGTCTTT
GATGCCAGCCAGAACGCCGTCAAGTTAGTCAGCGAAGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGG
CAACCGGTTGTTCGTGGGGGTGAGCAACCCAACCCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAATCTGGTGG
TGGAGCCGATCCTGGTCGATGAGGACCAAATCCGTCGCACCTTGGAGCAGTGGCAGGCGAGCAATGCGGCGCTCGGCTCG
TCGCTCGGTGACGACGATGACGACATGGGGGATCTGGACGTCTCCTCCGGGGACGAGGACATGGGCGCCGGCGGGGATTC
CGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTTAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAG
CCTCCGACATCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGCTACTGAAGAATGTGGCC
AAGGCGCCGGTGAAGCTGAGCCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCTGAGAAGCGGGT
GCCGCAGGATGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTGAGCACCTTGCCGACCCTGT
TCGGCGAGAAGGTGGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTAGGCTACGAGGCGGAT
CAGCAAAAGCTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGTAAGAC
GGTGTCGTTGTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTCGAAATCC
GCTTGCCTGGTGTGAATCAGGTGCAGCAGAACGTGAAGCGCGGCATGACGTTTGCCGCTGCGCTGCGCTCGTTCCTGCGC
CAGGATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCAGAGATTGCAATCAAGGCGGCGCAGACCGG
TCACATGGTGTTGTCGACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATTGCGCCGT
ACAACATCACTTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAATAACTGCAAGCGCAAGGCA
CCGCTGCCGGAGCACGCGCTGTTGGCCGAAGGGTTCACCGCTGAGCAGATCGCTGCAGGAATCGAACTGTACGAGGCGGT
TGGTTGCGACGAATGCACAGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCG
CAATCGTACTGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCAGCGCAGAGGATCGGCATACGCGATTTGCGGCAGTCG
GCGCTGGTGAAGGCGGCGCATGGAGTGACGAGCCTGGCTGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(204-471)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

97.762

0.544
  pilB Acinetobacter baylyi ADP1

54.577

97.762

0.534
  pilB Legionella pneumophila strain ERS1305867

52.197

97.935

0.511
  pilB Vibrio cholerae strain A1552

48.691

98.623

0.48
  pilF Neisseria gonorrhoeae MS11

48.239

97.762

0.472
  pilB Vibrio campbellii strain DS40M4

45.694

97.935

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

97.074

0.441
  pilF Thermus thermophilus HB27

40.691

89.673

0.365

Multiple sequence alignment