Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DW350_RS16435 Genome accession   NZ_CP031416
Coordinates   3466100..3467812 (-) Length   570 a.a.
NCBI ID   WP_115719975.1    Uniprot ID   -
Organism   Gallaecimonas mangrovi strain HK-28     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3461100..3472812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DW350_RS16405 ampD 3461822..3462364 (-) 543 WP_115719970.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DW350_RS16410 yacG 3462431..3462604 (-) 174 WP_115719971.1 DNA gyrase inhibitor YacG -
  DW350_RS16415 - 3462614..3463351 (-) 738 WP_115719972.1 cell division protein ZapD -
  DW350_RS16420 coaE 3463383..3463985 (-) 603 WP_115719973.1 dephospho-CoA kinase -
  DW350_RS16425 pilD 3463982..3464845 (-) 864 WP_192954719.1 prepilin peptidase Machinery gene
  DW350_RS16430 pilC 3464852..3466090 (-) 1239 WP_115719974.1 type II secretion system F family protein Machinery gene
  DW350_RS16435 pilB 3466100..3467812 (-) 1713 WP_115719975.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DW350_RS16440 - 3467840..3468271 (-) 432 WP_115719976.1 pilin -
  DW350_RS16445 nadC 3468601..3469452 (+) 852 WP_115719977.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DW350_RS16450 - 3469496..3470347 (-) 852 WP_115719978.1 universal stress protein -
  DW350_RS16455 - 3470359..3471834 (-) 1476 WP_115719979.1 SulP family inorganic anion transporter -
  DW350_RS16460 - 3471876..3472412 (-) 537 WP_115719980.1 cytochrome b -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63015.54 Da        Isoelectric Point: 6.4358

>NTDB_id=306616 DW350_RS16435 WP_115719975.1 3466100..3467812(-) (pilB) [Gallaecimonas mangrovi strain HK-28]
MSGFLPVAKGTLALLLRRGKISQATVEELLKENNSGLIPLFDVLKKSELTADAFVHFLSEQYGLPVFDLTKFDLDSIPST
LLNEQLIQKHSVLPLFKRQNRIFIATADPSNQAALEDFRFNLRLPTEPVLVNADELAKGIDKLLEKATGALDIGDVNDSE
LADLAVEDSEDREPELATSSKDDDAPIVIYINKLLMDAIRKGASDLHFEPYEKTYRVRFRIDGILHEIAAPPVSVANRLS
ARIKVMSKLDIAERRIPQDGRIKLRLSRKKAIDFRVSTLPTLWGEKVVMRILDSSSAQLGIDMLGYEPEQKRLYLEALHK
PQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLPGVNQVQIHTKAGMTFASALRAFLRQDPDIIMVGEI
RDLETAEISIKAAQTGHLVLSTLHTNSASETLTRLVNMGVPPYNIASSVTLVIAQRLGRRLCPVCKQPEHLPKDELLRQG
FTPRQIDEGFTLYRPVGCSECTGGYKGRVGIYETMPMTEALAKIIMEGGNSLDIAAQAQREGIKNLRQTGLEKARQGVTS
LAEVNRVTSY

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=306616 DW350_RS16435 WP_115719975.1 3466100..3467812(-) (pilB) [Gallaecimonas mangrovi strain HK-28]
ATGTCAGGATTCCTACCCGTTGCCAAAGGTACGCTTGCCCTGCTGTTAAGGCGGGGCAAGATTTCGCAGGCAACGGTTGA
GGAATTACTGAAGGAAAATAATTCCGGGTTGATCCCGCTGTTTGATGTTTTAAAAAAGTCTGAACTAACTGCCGATGCCT
TTGTGCATTTTCTGTCAGAGCAATACGGCCTGCCGGTTTTTGATTTAACCAAGTTCGACCTCGATTCCATACCCAGTACC
TTGTTAAACGAGCAGTTGATTCAAAAGCACTCGGTGCTGCCGCTGTTTAAAAGGCAAAACCGCATTTTTATTGCCACCGC
TGACCCTTCCAACCAAGCCGCATTAGAAGATTTTCGCTTTAATTTGCGCCTACCCACCGAGCCAGTATTGGTCAACGCCG
ATGAACTGGCGAAAGGCATTGATAAACTGCTTGAAAAGGCAACCGGCGCCCTGGATATCGGCGATGTAAACGATTCAGAG
CTGGCCGACCTTGCGGTTGAAGATAGCGAAGACAGAGAGCCGGAACTTGCTACTTCGTCAAAAGATGATGATGCACCAAT
TGTCATTTACATCAATAAGTTACTGATGGATGCTATCCGTAAAGGCGCATCGGACCTGCACTTTGAGCCCTATGAAAAAA
CCTACCGGGTGCGGTTTCGGATTGATGGCATCTTGCATGAAATTGCCGCGCCGCCAGTCTCCGTGGCTAACCGGTTGTCG
GCCCGTATCAAGGTGATGTCCAAGCTCGACATCGCCGAGCGGCGTATTCCCCAAGATGGTCGTATCAAGCTTAGGCTGTC
GCGAAAAAAAGCCATCGATTTTCGGGTATCAACCTTACCCACCCTTTGGGGCGAAAAGGTGGTAATGCGTATTCTCGACT
CCAGCTCGGCGCAGCTAGGTATCGATATGCTGGGGTATGAGCCCGAACAAAAACGTCTTTATCTTGAAGCCCTACACAAG
CCGCAAGGCATGATATTGGTCACCGGGCCTACAGGTTCCGGGAAAACGGTATCGCTATACACCGGCCTTAATATTTTAAA
TACCGAAGAGCGCAATATTTCAACGGCCGAAGATCCGGTTGAAATTAACCTGCCAGGGGTTAACCAAGTACAAATTCACA
CCAAGGCCGGTATGACCTTTGCCTCGGCGCTACGGGCTTTTTTGCGCCAAGACCCAGACATCATCATGGTGGGGGAAATT
CGCGACCTAGAAACCGCTGAAATTTCGATAAAAGCGGCGCAAACCGGCCACTTGGTACTTTCTACCTTACACACCAACTC
GGCCTCTGAAACCCTGACTCGATTAGTGAACATGGGGGTGCCGCCCTACAACATTGCCTCCAGCGTTACCCTGGTTATTG
CCCAGCGCCTGGGCCGGCGTTTGTGTCCGGTCTGTAAGCAGCCTGAGCACTTACCCAAAGATGAGTTACTACGCCAAGGT
TTTACCCCCCGGCAAATTGACGAAGGTTTTACCCTGTACCGCCCGGTTGGGTGCAGCGAGTGCACCGGCGGCTATAAAGG
CCGGGTTGGGATCTACGAGACCATGCCAATGACCGAAGCGCTGGCTAAAATCATTATGGAAGGGGGGAATTCTCTTGATA
TTGCCGCCCAAGCGCAGCGAGAAGGCATTAAAAACTTGCGCCAAACCGGTTTAGAAAAAGCGCGCCAAGGGGTTACCTCG
TTAGCTGAGGTGAACCGTGTCACCAGCTACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.401

100

0.572
  pilB Acinetobacter baumannii D1279779

60.113

92.807

0.558
  pilB Legionella pneumophila strain ERS1305867

56.903

94.035

0.535
  pilB Vibrio cholerae strain A1552

52.993

99.649

0.528
  pilB Vibrio campbellii strain DS40M4

52.482

98.947

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

52.406

98.421

0.516
  pilF Neisseria gonorrhoeae MS11

48.142

99.123

0.477
  pilF Thermus thermophilus HB27

40.564

99.474

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.187

97.544

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

40.691

91.404

0.372

Multiple sequence alignment