Detailed information NTG0306614

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DW350_RS16425
• Genome accession: NZ_CP031416
• Coordinates: 3463982..3464845 (-)
• Length: 287 a.a.
• NCBI ID: WP_192954719.1
• Uniprot ID: -
• Organism: Gallaecimonas mangrovi strain HK-28
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3458982..3469845

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DW350_RS16395	pdhR	3460114..3460893 (-)	780	WP_115720669.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
DW350_RS16400	ampE	3461025..3461825 (-)	801	WP_115719969.1	regulatory signaling modulator protein AmpE	-
DW350_RS16405	ampD	3461822..3462364 (-)	543	WP_115719970.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
DW350_RS16410	yacG	3462431..3462604 (-)	174	WP_115719971.1	DNA gyrase inhibitor YacG	-
DW350_RS16415	-	3462614..3463351 (-)	738	WP_115719972.1	cell division protein ZapD	-
DW350_RS16420	coaE	3463383..3463985 (-)	603	WP_115719973.1	dephospho-CoA kinase	-
DW350_RS16425	pilD	3463982..3464845 (-)	864	WP_192954719.1	prepilin peptidase	Machinery gene
DW350_RS16430	pilC	3464852..3466090 (-)	1239	WP_115719974.1	type II secretion system F family protein	Machinery gene
DW350_RS16435	pilB	3466100..3467812 (-)	1713	WP_115719975.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DW350_RS16440	-	3467840..3468271 (-)	432	WP_115719976.1	pilin	-
DW350_RS16445	nadC	3468601..3469452 (+)	852	WP_115719977.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 287 a.a.
• Molecular weight: 32174.45 Da
• Isoelectric Point: 8.3855

>NTDB_id=306614 DW350_RS16425 WP_192954719.1 3463982..3464845(-) (pilD) [Gallaecimonas mangrovi strain HK-28]
MQWMDLLGREPLWLAVFVFVLGMLVGSFLNVVIYRLPVMLERRWKRESRELLALEFHDSLPPFNLMVPRSHCPKCHAKVA
WYDNIPLISWLLLRGRCRHCQTKISARYPLIELITAVCSAVVALHFGASFTLLAALFFTWMLIALTFIDADTMLLPDDLT
LPLLWAGLLVSCFDGGVIPTQAIVGAACGYLSLWAVYWLFKLATGKEGMGYGDFKLLAALGAWLGPWMLPQIILLSSVFG
VLWGIVSAVSHKRGSHPMPFGPFLAMAGWVALLWGQSINQWYLGTFS

Nucleotide

• Length: 864 bp

>NTDB_id=306614 DW350_RS16425 WP_192954719.1 3463982..3464845(-) (pilD) [Gallaecimonas mangrovi strain HK-28]
ATGCAATGGATGGATTTATTGGGCCGCGAGCCATTGTGGTTGGCGGTTTTTGTGTTTGTGCTGGGCATGCTGGTAGGCAG
CTTTTTAAACGTGGTTATCTACCGCTTGCCGGTGATGCTAGAACGGCGCTGGAAACGTGAAAGCCGCGAATTATTGGCGC
TGGAGTTTCACGATTCCCTTCCTCCTTTTAACCTAATGGTACCGCGTAGCCATTGCCCGAAATGCCATGCCAAAGTGGCG
TGGTACGACAATATCCCGCTAATAAGTTGGTTATTGCTGCGTGGCCGCTGCCGGCATTGCCAAACCAAGATCTCGGCACG
CTATCCGCTCATTGAGTTGATCACTGCTGTTTGCTCAGCGGTGGTCGCGCTGCATTTTGGTGCCAGTTTTACGCTGTTGG
CGGCGCTCTTTTTTACCTGGATGCTTATTGCCCTGACCTTTATCGACGCGGACACCATGCTACTGCCGGATGACCTGACC
TTGCCGCTACTATGGGCGGGGTTATTGGTTAGCTGTTTTGATGGCGGCGTGATACCGACGCAAGCCATTGTTGGCGCTGC
CTGTGGTTATTTATCGCTTTGGGCCGTCTATTGGCTGTTTAAACTTGCCACCGGTAAAGAAGGTATGGGTTACGGCGACT
TTAAATTGCTGGCGGCATTGGGCGCTTGGCTCGGCCCTTGGATGCTGCCGCAAATTATATTGTTGTCTTCGGTGTTTGGT
GTGCTTTGGGGCATTGTGAGTGCCGTGAGCCACAAACGGGGTAGCCACCCCATGCCCTTTGGTCCCTTCCTGGCTATGGC
TGGGTGGGTTGCTTTACTTTGGGGGCAAAGTATTAACCAGTGGTATTTAGGAACCTTTAGCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	55.017	100	0.554
pilD	Vibrio cholerae strain A1552	53.659	100	0.537
pilD	Acinetobacter nosocomialis M2	54.182	95.819	0.519
pilD	Acinetobacter baumannii D1279779	53.455	95.819	0.512
pilD	Neisseria gonorrhoeae MS11	53.257	90.941	0.484