Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EW998_RS20990 Genome accession   NZ_CP035874
Coordinates   4322671..4324056 (+) Length   461 a.a.
NCBI ID   WP_001025156.1    Uniprot ID   -
Organism   Escherichia coli strain B2C     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4317671..4329056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW998_RS20965 aroP 4318219..4319592 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  EW998_RS20970 ampE 4319635..4320489 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  EW998_RS20975 ampD 4320486..4321037 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EW998_RS20980 nadC 4321125..4322018 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EW998_RS20985 pilA 4322221..4322661 (+) 441 WP_000360896.1 prepilin peptidase-dependent pilin Machinery gene
  EW998_RS20990 pilB 4322671..4324056 (+) 1386 WP_001025156.1 type II secretion system protein GspE Machinery gene
  EW998_RS20995 hofC 4324046..4325248 (+) 1203 WP_000157223.1 protein transport protein HofC -
  EW998_RS21000 guaC 4325283..4326326 (-) 1044 WP_001217351.1 GMP reductase -
  EW998_RS21005 - 4326482..4326526 (-) 45 WP_120795372.1 protein YacM -
  EW998_RS21010 coaE 4326551..4327171 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  EW998_RS21015 zapD 4327171..4327914 (+) 744 WP_001194731.1 cell division protein ZapD -
  EW998_RS21020 yacG 4327924..4328121 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  EW998_RS21025 mutT 4328228..4328617 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50616.31 Da        Isoelectric Point: 6.6611

>NTDB_id=306263 EW998_RS20990 WP_001025156.1 4322671..4324056(+) (pilB) [Escherichia coli strain B2C]
MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSAQEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=306263 EW998_RS20990 WP_001025156.1 4322671..4324056(+) (pilB) [Escherichia coli strain B2C]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCATGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCAGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCAGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCAGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCAGCAGCAAGGGGAGCCCATCCATATTCCAGTCAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

42.769

100

0.449
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Acinetobacter baylyi ADP1

39.957

100

0.406
  pilB Vibrio cholerae strain A1552

46.465

85.9

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.891

83.731

0.393
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

40.831

88.72

0.362